1887

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Volume 3, Issue 3

The Influence Of Certain Lipids And Proteins On The Effect Of Tetanus Toxin In Mice Free

Abstract

SUMMARY

The effect of a tubercle bacillary lipid, lecithin and some proteins on the activity of tetanus toxin was measured by injecting them into mice with graded doses of the toxin and noting the survival times of the animals.

When the tetanus toxin was given as a set volume of a dilution in saline containing lipid or protein, it gave shorter survival times and was lethal at lower concentrations than when it was given as the same dilution in saline alone. However, the addition of lipid or protein to the toxin already diluted in saline alone had no influence on the survival times of the mice. Injection of the corresponding amount of toxin as a measured volume of the initial dilution gave even shorter survival times.

Tetanus toxin is adsorbed or inactivated at glass surfaces and is inactivated at gas-liquid interfaces. The losses from these causes are reduced in the presence of lipid or protein in the diluent or by treating the glass surfaces with these materials before they are brought into contact with diluted toxin.

The apparent activity of the lipids or proteins in activating the toxin is due to the reduction of such losses. No evidence was found of any direct effect on the activity of the toxin.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1970
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1970-08-01
2024-04-24
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References

  1. Abel J. J., Evans E. A., Hampil Bettylee. 1936 Bull. Johns Hopkins Hosp 59:307
     [Google Scholar]
  2. Anderson R. J. 1927 J. Biol. Chem 74:525
     [Google Scholar]
  3. Benzoni G. 1947 Riv. Ist. sieroter. ital 22:70
     [Google Scholar]
  4. Condrea P., Poenaru Hélène. 1933 C.r,. Séanc. Soc. Biol. Paris 112: 1482 and 1484
    [Google Scholar]
  5. Dernby K. G., Walbum L. E. 1923 Biochem,. Z 138:505
     [Google Scholar]
  6. Friedemann U., Hollander A. 1943 J. Immun 47:23
     [Google Scholar]
  7. Halter K. 1936 Z. Hyg. InfektKrankh 118:245
     [Google Scholar]
  8. van Heyningen W. E. 1950; Bacterial toxins. Oxford pp 19–23
     [Google Scholar]
  9. Istrati G. 1938 Zentbl. Bakt Abt I, Orig 143:106
     [Google Scholar]
  10. Largier J. F. 1956 J. Immun 76:393
     [Google Scholar]
  11. Maloney P. J., Taylor Edith M. 1932 Biochem,. J 26:1754
     [Google Scholar]
  12. Marie A. 1914 Annls Inst. Pasteur, Paris 28:1
     [Google Scholar]
  13. Mutermilch S., Belin M., Salamon E. 1932 C.r,. Séanc. Soc. Biol. Paris 111:558
     [Google Scholar]
  14. Mutermilch S., Belin M., Salamon E. 1933a Ibid 113:599
     [Google Scholar]
  15. Mutermilch S., Belin M., Salamon E. 1933b Ibid 114:1005
     [Google Scholar]
  16. Mutermilch S., Belin M., Salamon E. 1934 Ibid 116:1245
     [Google Scholar]
  17. Pillemer L. 1946 J. Immun 53:237
     [Google Scholar]
  18. Pillemer L., Wartman W. B. 1947 Ibid 55:277
     [Google Scholar]
  19. Pound A. W. 1955 J. Path. Bact 70:119
     [Google Scholar]
  20. Pound A. W. 1958 Ibid 75:55
     [Google Scholar]
  21. Pound A. W. 1970 Pathology in press
    [Google Scholar]
  22. Ramon G., Lémétayer E., Nicol L. 1938a C.r,. Séanc. Soc. Biol. Paris 127:1160
     [Google Scholar]
  23. Ramon G., Lémétayer E., Richou R., Nicol L. 1938b Ibid 127:1157
     [Google Scholar]
  24. Reed L. J., Muench H. 1938 Amer. J. Hyg 27:493
     [Google Scholar]
  25. Ricketts H. T., Kirk E. J. 1906 J. Infect. Dis 3:116
     [Google Scholar]
  26. Salamon E., Mutermilch S., Belin M. 1934 C.r,. Séanc. Soc. Biol. Paris 115:1476
     [Google Scholar]
  27. Smith Margaret Llewellyn. 1942–43 Bull. Hlth Org. L,. O,. N 10:104
     [Google Scholar]
  28. Sommer E. W., Sommer H. 1928 J. Infect. Dis 43:496
     [Google Scholar]
  29. Traub F. B., Hollander A., Friedemann U. 1946 J. Bad 52:169
     [Google Scholar]
  30. Zuger B., Hollander A., Friedemann U. 1939 J. Infect. Dis 65:86
     [Google Scholar]
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The Influence Of Certain Lipids And Proteins On The Effect Of Tetanus Toxin In Mice
J. Med. Microbiol. 3, 411 (1970); https://doi.org/10.1099/00222615-3-3-411
/content/journal/jmm/10.1099/00222615-3-3-411
/content/journal/jmm/10.1099/00222615-3-3-411
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