Probably because is a well-defined species whose recognition is rarely difficult, no recent attempts have been made to clarify the present uncertainties about its gelatinolytic and proteolytic activity. In the present study of 71 strains of the organism, all of them were shown to produce gelatinase, and none was proteolytic.

A proportion of the strains (49 of 71) produced a fibrinolytic enzyme, which behaved like a kinase.

A proportion of the strains (40 of 71) produced a deoxyribonuclease.

None of the strains produced phosphatase.

All 71 strains produced an intense diffuse opacity on milk agar, and complete precipitation of casein in milk broth due to the production of a rennin-like enzyme. This enzyme was present in culture-supernatants, was active under both aerobic and anaerobic conditions, and was specifically inhibited by tetanus antitoxic sera. With the exception of types A and B, the reactions of all other clostridia tested on milk agar and in milk broth were dissimilar to those of .

Commercial horse tetanus antitoxic serum inhibits the swarming growth of in plate cultures. Advantage may be taken of this in the separation of other anaerobic organisms from mixtures with .


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