A Study Of The Localisation Of A Protein Antigen In The Chicken Spleen And Its Relation To The Formation Of Germinal Centres Free

Abstract

SUMMARY

Chickens given an intravenous injection of human serum albumin (HSA) produced a rapid rise of antibody to a peak at 8–9 days, followed by a rapid decline. Plasma cells containing anti-HSA appeared in the spleen at 24 hr after injection. Evidence is presented that early produced antibody causes the localisation of antigen (HSA), presumably as complexes, to cells in the white pulp of the spleen at the periphery of ellipsoids (Schweigger-Seidel sheaths) by 24–32 hr. Inspection of a series of tissue sections shows that antigen-bearing cells appear to migrate through the white pulp and subsequently to appear (88 hr–6 days) as within germinal centres, which originated in the angle between the diverging penicillary arterioles at their point of origin from the central arteriole of the white pulp. A hypothesis is advanced that accounts for the formation of germinal centres by a process of progressive capture and aggregation of lymphocytes at the surface of antigen-bearing dendritic cells. This process is discussed in relation to the progressive increase of avidity in antibody synthesised later in the immunological response or after a secondary stimulus.

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1970-02-01
2024-03-28
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References

  1. Ada G. L., Nossal G. J. V., Pye J. 1964 Austral. J. Exp. Biol. Med. Sci 42:295
    [Google Scholar]
  2. Campbell D. H., Garvey June S., Cremer Natalie E., Sussdoar D. M. 1964 Methods in immunology New York: p 203
    [Google Scholar]
  3. Coons A. H., Creech H. J., Joes R. N., Berliner E. 1942 J. Immun 45:159
    [Google Scholar]
  4. Coons A. H., Kaplan M. H. 1950 J. Exp. Med 91:1
    [Google Scholar]
  5. Coons A. H., Leduc Elizabeth H., Connolly Jeanne M. 1955 Ibid 102:49
    [Google Scholar]
  6. Coons A. H., Leduc Elizabeth H., Kaplan M. H. 1951 Ibid 93:173
    [Google Scholar]
  7. Eisen H. N., Siskind G. W. 1964 Biochemistry 3:996
    [Google Scholar]
  8. Farr R. S. 1958 J. Infect. Div 103:239
    [Google Scholar]
  9. French Valentine I., Wilkinson P. C., White R. G. 1969; Lymphatic tissue and germinal centers in immune response. ed. by Fiore-Donati L., Hanna M. G. New York: p 211
    [Google Scholar]
  10. Hunter W. M., Greenwood F. C. 1962 Nature, Lond 194:495
    [Google Scholar]
  11. Kabat E. A., Mayer M. M. 1961; Experimental immunochemistry. 2nd ed Springfield, Ill pp 71–73
    [Google Scholar]
  12. McDevitt H. O., Askonas Brigitte A., Humphrey J. H., Schechter I., Sela M. 1966 Immunology 11:337
    [Google Scholar]
  13. Marshall A. H. E. 1956; An outline of the cytology and pathologyof the reticular tissue. Edinburgh pp 23 et seqq:
    [Google Scholar]
  14. Nossal G. J. V., Ada G. L., Austin Caroline M. 1964 Austral. J. Exp. Biol. Med. Sci 42:311
    [Google Scholar]
  15. Sell S., Gell P. G. H. 1965 J. Exp. Med 122:423
    [Google Scholar]
  16. White R. G. 1958 Nature, Loud 182:1383
    [Google Scholar]
  17. White R. G. 1963 Ciba Fdn Study Grps 16:6
    [Google Scholar]
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