1887

Abstract

SUMMARY

This paper describes the isolation and characterisation of a new mycoplasma species for which the name is proposed. Many strains of this glucose-fermenting organism were isolated in an enriched glucose calf-serum medium from pneumonic lungs of calves, but not from healthy lungs of calves or cows. It could be isolated in medium free of known bacterial inhibitors, and when subcultured in this medium showed no evidence of reversion to bacterial forms.

The general characteristics of these strains were those of mycoplasmas; these included cultural and metabolic characters, morphological appearance in stained films and electron micrographs, growth inhibition by specific antiserum and a DNA base composition of 28.5 per cent. GC. However, the group showed some unusual features, including failure to pass readily through membrane filters of 450 nm pore diameter, poor growth in conventional mycoplasma media and production of atypical colonies on GS agar medium.

The serological identity of strains classified as was demonstrated by growth and metabolic inhibition tests. They were serologically distinct from other recognised bovine mycoplasmas and from a wide range of mycoplasmas from other sources.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-3-1-111
1970-02-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/3/1/medmicro-3-1-111.html?itemId=/content/journal/jmm/10.1099/00222615-3-1-111&mimeType=html&fmt=ahah

References

  1. Anderson D. R., Barile M. F. 1965 J. Bact 90:180
    [Google Scholar]
  2. Bak A. L., Black F. T. 1968 Nature, Lond 219:1044
    [Google Scholar]
  3. Chanock R. M., Hayflick L., Barile M. F. 1962 Proc. Natn. Acad. Sci USA 48:41
    [Google Scholar]
  4. Clyde W. A. 1964 J. Immun 92:958
    [Google Scholar]
  5. Davies G. 1967 J. Comp. Path. Ther 77:353
    [Google Scholar]
  6. Edward D. G. Ff., Freundt E. A. 1969 J. Gen. Microbiol 57:391
    [Google Scholar]
  7. Furness G., Pipes Florence J., Mcmurtrey M. J. 1968 J. Infect. Dis 118:7
    [Google Scholar]
  8. Goodwin R. F. W., Pomeroy P., Whittlestone Annette P. 1965 Vet. Rec 77:1247
    [Google Scholar]
  9. Goodwin R. F. W., Pomeroy Annette P., Whittlestone P. 1967 J. Hyg Camb 65:85
    [Google Scholar]
  10. Gourlay R. N. 1968 Res. Vet. Sci 9:376
    [Google Scholar]
  11. Gourlay R. N. 1969 Vet. Rec 84:229
    [Google Scholar]
  12. Günther H., Hubrig T., Martin J., Schimmel D. 1967 Arch. exp. VetMed 21:7
    [Google Scholar]
  13. Hill L. R. 1966 J. Gen. Microbiol 44:419
    [Google Scholar]
  14. Klineberger-Nobel Emmy. 1962; Pleuropneumonia-like organisms (PPLO): Mycoplasmataceae. London and New York
    [Google Scholar]
  15. Leach R. H. 1967 Ann. N. Y. Acad. Sci 143:305
    [Google Scholar]
  16. Madoff Sarabelle. 1960 Ibid 79: art. 10, p 383
    [Google Scholar]
  17. Mare C. J., Switzer N. P. 1965 Vet. Med 60:841
    [Google Scholar]
  18. Marmur J. 1961 J. Molec. Biol 3:208
    [Google Scholar]
  19. Marmur J., Doty P. 1962 Ibid 5:109
    [Google Scholar]
  20. Owen R. J., Lapage S. P., Hill L. R. 1969 Biopdlymers in press
    [Google Scholar]
  21. Razin S., Rottem S. 1967 J. Bact 94:1807
    [Google Scholar]
  22. Reuss K., Plescher C., Hulser D., Herzberg K. 1967 Zentbl. Bakt. Parasitkde abt. 1, orig 203:121
    [Google Scholar]
  23. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. 1966 J. Hyg camb 64:91
    [Google Scholar]
  24. Taylor-Robinson D., Berry D. M. 1969 J. Gen. Microbiol 55:127
    [Google Scholar]
  25. Van Boven C. P. A., Ensering Henriette L., Hijmans W. 1968 Ibid 52:403
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-3-1-111
Loading
/content/journal/jmm/10.1099/00222615-3-1-111
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error