1887

Abstract

The susceptibility of 130 clinical isolates of gram-positive cocci to a wide range of antimicrobial agents was assessed by ATP bioluminescence in a 4-h test. ATP assays were performed on a novel luminometer, the Amerlite Analyser, which measures luminescence from microtitration trays. For most organisms tested, there was good correlation (>90%) with conventional MIC values estimated on 18-h cultures. However, a problem was found with detection of penicillin resistance in by the ATP method, 13% of strains showing major disagreement. Methicillin resistance of was shown reliably for most strains (94%) by ATP assay, provided they were incubated at 30°C. The Amerlite Analyser offers the potential for the development of a semi-automated antimicrobial susceptibility test, with a significant reduction in reagent costs when compared with previously described bioluminescence protocols.

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/content/journal/jmm/10.1099/00222615-29-4-277
1989-08-01
2019-10-18
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-29-4-277
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