1887

Abstract

Summary

The ability of RI restriction enzyme fragmentation patterns and of immunoblotting to differentiate methicillin-resistant isolates of were compared. All isolates examined were typable by both methods and the reproducibility of each was excellent. Immunoblotting differentiated eight types and DNA restriction patterns four. The former technique was of value in characterising methicillin-resistant isolates of and controlling an outbreak due to them.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-29-4-255
1989-08-01
2024-06-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/29/4/medmicro-29-4-255.html?itemId=/content/journal/jmm/10.1099/00222615-29-4-255&mimeType=html&fmt=ahah

References

  1. Burnie J. P., Matthews R. C. 1987; Immunoblot analysis: a new method for fingerprinting hospital pathogens. Journal of Immunological Methods 100:41–46
    [Google Scholar]
  2. Burnie J. P., Lee W. 1988; A comparison of DNA and immunoblot fingerprinting of the S II biotype of coagulase negative staphylococci. Epidemiology and Infection 101:203–212
    [Google Scholar]
  3. Feng-Ying C. 1986; Persistence of cholera in the United States: Isolation of Vibrio cholera 0.l from a patient with diarrhoea in Maryland. Journal of Clinical Microbiology 23:624–626
    [Google Scholar]
  4. Gaston M. A. 1988; Evaluation of electrophoretic methods for typing methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 26:189–197
    [Google Scholar]
  5. Jordens J. Z., Hall L. M. C. 1988; Characterisation of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA. Journal of Medical Microbiology 27:117–123
    [Google Scholar]
  6. Krikler S. J., Pennington T. H., Petrie D. 1986; Typing of strains of Staphylococcus aureus by Western blot analysis of culture supernates. Journal of Medical Microbiology 21:169–171
    [Google Scholar]
  7. Lacey R. W., Chopra I. 1975; The effect of plasmid carriage on the virulence of Staphylococcus aureus. Journal of Medical Microbiology 8:137–147
    [Google Scholar]
  8. Lacey R. W., Barr K. W., Barr V. E., Inglis T. J. 1986; Properties of methicillin-resistant Staphylococcus aureus colonising patients in a burns unit. Journal of Hospital Infection 7:137–148
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  10. Langenberg W., Rauws E. A. J., Widjojokusumo A., Tytgat G. N. J., Zanen H. C. 1986; Identification of Campylobacter pylori isolates by restriction endonuclease DNA analysis. Journal of Clinical Microbiology 24:414–417
    [Google Scholar]
  11. Lee W., Bumie J. P. 1988; Fingerprinting methicillin-resistant Staphylococcus aureus by the immunoblot technique. Journal of Medical Microbiology 25:261–268
    [Google Scholar]
  12. Marples R. R., Richardson J. F., de Saxe M. J. 1986; Bacteriological characters of strains of Staphylococcus aureus submitted to a reference laboratory related to methicillin-resistance. Journal of Hygiene 96:217–223
    [Google Scholar]
  13. Parker M. T. 1972; Phage-typing of Staphylococcus aureus. In Norris J. R., Ribbons D. W. (eds) Methods in microbiology 7B Academic Press; London:B1–28
    [Google Scholar]
  14. Report of Combined working party of the Hospital Infection Society and British Society for Antimicrobial Chemotherapy 1986; Guidelines for the control of epidemic methicillin resistant Staphylococcus aureus. Journal of Hospital Infection 7:193–201
    [Google Scholar]
  15. Scherer S., Stevens D. A. 1987; Application of DNA typing methods to epidemiology and taxonomy of Candida species. Journal of Clinical Microbiology 25:675–679
    [Google Scholar]
  16. Stephenson J. R., Crook S. J., Tabaqchali S. 1986; New method for typing Staphylococcus aureus resistant to methicillin based on sulphur-35 methionine labelled proteins: its application in an outbreak. British Medical Journal 293:581–583
    [Google Scholar]
  17. Stokes E. J., Waterworth P. M. 1972; Antibiotic sensitivity tests by diffusion methods. Association of Clinical Pathologists Broadsheet No. 55
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-29-4-255
Loading
/content/journal/jmm/10.1099/00222615-29-4-255
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error