1887

Abstract

Surmmary

The SOS DNA repair system is induced in bacteria treated with 4-quinolones. However, whether the response exacerbates or repairs the damage caused by these drugs is still unclear. The and the mutations impair recombination repair and render bacteria unable to induce the SOS response when treated with nalidixic acid or other agents that affect DNA synthesis. However, UV treatment induces the SOS response in mutants but not in mutants. Both these mutants are hypersensitive to nalidixic acid and, therefore, either recombination repair or SOS repair would appear to repair DNA damage caused by the drug. However, since the mutation (which also renders bacteria incapable of inducing the SOS response without affecting recombination repair) had no effect on the susceptibility of bacteria to nalidixic acid, the SOS response neither contributes to nor repairs DNA damage caused by the drug. Consequently, it would seem that the hypersensitivity of the and mutants to nalidixic acid is due to their deficiency in recombination repair. This view was confirmed by testing a mutant that is recombination-repair proficient but SOS repair-deficient and finding it to be no more sensitive to nalidixic acid than its parent. Thus it would appear that, although induced by nalidixic acid treatment, the SOS DNA repair system does not play any role in bacterial responses to the damage caused by the drug. In contrast, the recombination repair system does repair damage caused by nalidixic acid.

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1989-06-01
2022-01-18
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References

  1. Bailone A., Sommer S., Devoret R. 1985; Mini-F plasmid-induced SOS signal in Escherichia coli is RecBC-dependent. Proceedings of the National Academy of Sciences of the USA 82:5973–5977
    [Google Scholar]
  2. Benbrook D. M., Miller R. V. 1986; Effects of Norfloxacin on DNA metabolism in Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy 29:1–6
    [Google Scholar]
  3. Blanco M., Herrera G., Aleixandre V. 1986; Different efficiency of UmuCDC MucAB proteins in UV light induced mutagenesis in Escherichia coli. Molecular and General Genetics 205:234–239
    [Google Scholar]
  4. Chaudhury A. M., Smith G. R. 1985; Role of Escherichia coli RecBC enzyme in SOS induction. Molecular and General Genetics 201:525–528
    [Google Scholar]
  5. Chow R. T., Dougherty T. J., Fraimow H. S., Beilin E. Y., Miller M. H. 1988; Association between early inhibition of DNA synthesis and the MICs and MBCs of carboxyquinolone antimicrobial agents for wild-type and mutant (gyrA nfxB (ompF) acrA) Escherichia coli K12. Antimicrobial Agents and Chemotherapy 32:1113–1118
    [Google Scholar]
  6. Crumplin G. C., Smith J. T. 1975; Nalidixic acid; an antibacterial paradox. Antimicrobial Agents and Chemotherapy 8:251–261
    [Google Scholar]
  7. Crumplin G. C., Kenwright M., Hirst T. 1984; Investigations into the mechanism of action of the antibacterial agent norfloxacin. Journal of Antimicrobial Chemotherapy 13: suppl B9–23
    [Google Scholar]
  8. Dietz W. H., Cook T. M., Goss W. A. 1966; Mechanism of action of nalidixic acid on Escherichia coli:III Conditions required for lethality. Journal of Bacteriology 91:768–773
    [Google Scholar]
  9. Drlica K. 1984; Biology of bacterial deoxyribonucleic acid topoisomerases. Microbiological Reviews 48:273–289
    [Google Scholar]
  10. Goss W. A., Dietz W. H., Cook T. M. 1964; Mechanism of action of nalidixic acid on Escherichia coli. Journal of Bacteriology 88:1112–1118
    [Google Scholar]
  11. Goss W. A., Dietz W. H., Cook T. M. 1965; Mechanism of action of nalidixic acid on Escherichia coli II. Inhibition of deoxyribonucleic acid synthesis. Journal of Bacteriology 89:1068–1074
    [Google Scholar]
  12. Gudas L. J., Pardee A. B. 1976; DNA synthesis and the induction of protein X in Escherichia coli. Journal of Molecular Biology 101:459–477
    [Google Scholar]
  13. Little J. W. 1984; Autodigestion of lex A and phage lambda repressors. Proceedings of the National Academy of Sciences of the USA 81:1375–1379
    [Google Scholar]
  14. Little J. W., Mount D. W. 1982; The SOS regulatory system of Escherichia coli. Cell 29:11–22
    [Google Scholar]
  15. McDaniel L. S., Rogers L. H., Hill W. E. 1978; Survival of recombination-deficient mutants of Escherichia coli during incubation with nalidixic acid. Journal of Bacteriology 134:1195–1198
    [Google Scholar]
  16. Peterson K. R., Ossanna N., Thliveris A. T., Ennis D. G., Mount D. W. 1988; Derepression of specific genes promotes DNA repair and mutagenesis in Escherichia coli. Journal of Bacteriology 170:1–4
    [Google Scholar]
  17. Phillips I. 1987; Bacterial mutagenicity and the 4-quinolones. Journal of Antimicrobial Chemotherapy 20:771–773
    [Google Scholar]
  18. Phillips I., Culebras E., Moreno F., Baquero P. 1987; Induction of the SOS response by new 4–quinolones. Journal of Antimicrobial Chemotherapy 20:631–638
    [Google Scholar]
  19. Piddock L. J. V., Wise R. 1987; Induction of the SOS response in Escherichia coli by 4–quinolone antimicrobial agents. FEMS Microbiology Letters 41:289–294
    [Google Scholar]
  20. Ratcliffe N. T., Smith J. T. 1984; Ciprofloxacin and ofloxacin exhibit a rifampicin-resistant bactericidal mechanism not detectable in other 4-quinolone antibacterial agents. Journal of Pharmacy and Pharmacology 36:59
    [Google Scholar]
  21. Slilaty S. N., Rupley J. A., Little J. W. 1986; Intramolecular cleavage of lex A and phage lambda repressors:dependence of kinetics on repressor concentration, pH, temperature and solvent. Biochemistry 25:6866–6875
    [Google Scholar]
  22. Smith G. R. 1981; DNA supercoiling:Another level for regulating gene expression. Cell 24:599–600
    [Google Scholar]
  23. Smith J. T. 1984; Awakening the slumbering potential of the 4– quinolone antibacterials. Pharmaceutical Journal 233:299–305
    [Google Scholar]
  24. Walker G. C. 1984; Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiological Reviews 48:60–93
    [Google Scholar]
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