Outer-membrane proteins (OMPs) of strains of O1 and non-O1 serovars were studied. Marked similarity was found in the OMP profiles of different O1 strains but the OMP profile of a non-O1 strain was somewhat different. Antigenic relatedness between the OMPs of different strains was established by enzyme-linked immunosorbent assay (ELISA). Immunoblotting experiments demonstrated that at least two OMPs of 36 and 25-26 Kda were immunogenic and common to strains of O1 and non-O1 serovars. Antiserum raised against the outer membrane of a strain, and rendered specific for its OMP by absorption with lipopolysaccharide, inhibited the intestinal adhesion of the homologous and heterologous strains of irrespective of their biotype, serotype and serovar. Furthermore, antiserum to OMPs induced passive protection against vibrio challenge in rabbit ileal loop experiments. These results suggest that the OMPs may be useful in immunoprophylaxis against cholera.


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