1887

Abstract

Summary

Outer-membrane proteins (OMPs) of strains of O1 and non-O1 serovars were studied. Marked similarity was found in the OMP profiles of different O1 strains but the OMP profile of a non-O1 strain was somewhat different. Antigenic relatedness between the OMPs of different strains was established by enzyme-linked immunosorbent assay (ELISA). Immunoblotting experiments demonstrated that at least two OMPs of 36 and 25-26 Kda were immunogenic and common to strains of O1 and non-O1 serovars. Antiserum raised against the outer membrane of a strain, and rendered specific for its OMP by absorption with lipopolysaccharide, inhibited the intestinal adhesion of the homologous and heterologous strains of irrespective of their biotype, serotype and serovar. Furthermore, antiserum to OMPs induced passive protection against vibrio challenge in rabbit ileal loop experiments. These results suggest that the OMPs may be useful in immunoprophylaxis against cholera.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-29-1-33
1989-05-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/29/1/medmicro-29-1-33.html?itemId=/content/journal/jmm/10.1099/00222615-29-1-33&mimeType=html&fmt=ahah

References

  1. Attridge S. R., Rowley D. 1983; Prophylactic significance of the non lipopolysaccharide antigens of Vibrio cholerae. Journal of Infectious Diseases 148:931–939
    [Google Scholar]
  2. Blake P. A., Weaver R. D., Hollis D. G. 1980; Diseases of humans (other than cholera) caused by vibrios. Annual Review of Microbiology 34:341–367
    [Google Scholar]
  3. Buchanan T. M., Pearce W. A. 1979; Pathogenic aspects of outer membrane components of gram-negative bacteria. In Inouye M. (ed) Bacterial outer membranes, biogenesis and functions John Wiley and Sons, Inc; New York, NY: pp 475–514
    [Google Scholar]
  4. Chitnis D. S., Sharma K. D., Kamat R. S. 1982; Role of somatic antigen of Vibrio cholerae in adhesion to intestinal mucosa. Journal of Medical Microbiology 15:53–61
    [Google Scholar]
  5. Dasgupta C. 1986; Studies on the protective immune mechanism in cholera with special reference to intestinal adhering and hemagglutinating factors of Vibrio cholerae. PhD Thesis University of Calcutta; India:
    [Google Scholar]
  6. Dasgupta C., Ghose A. C. 1988; Protective antigens of V. cholerae. In Talwar G. P. (ed) Progress in vaccinology vol 2 Springer Verlag; (in press)
    [Google Scholar]
  7. Datta-Roy K., Banerjee K., De S. P., Ghose A. C. 1986; Comparative study of expression of hemagglutinins, hemolysins, and enterotoxins by clinical and environmental isolates of non-Ol Vibrio cholerae in relation to their enteropathogenicity. Applied and Environmental Microbiology 52:875–879
    [Google Scholar]
  8. De S. N., Chatterje D. N. 1953; An experimental study of the mechanism of action of Vibrio cholerae on the intestinal mucous membrane. Journal of Pathology and Bacteriology 66:559–562
    [Google Scholar]
  9. Filip C., Fletcher G., Wulflf J. L., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  10. Finkelstein R. A. 1973; Cholera. CRC Critical Reviews in Microbiology 2:553–623
    [Google Scholar]
  11. Holmgren J., Svennerholm A.-M. 1977; Mechanism of disease and immunity in cholera, a review. Journal of Infectious Diseases 136: Suppl S105–S112
    [Google Scholar]
  12. Kabir S. 1980; Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae. Journal of Bacteriology 144:382–389
    [Google Scholar]
  13. Kabir S. 1983; Immunochemical properties of the major outer membrane protein of Vibrio cholerae. Infection and Immunity 39:452–455
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. Levine M. M. 1979; Immunity to cholera in man: relative role of antibacterial versus antitoxic immunity. Transactions of the Royal Society of Tropical Medicine and Hygiene73–39
    [Google Scholar]
  16. Mahin D. T., Lofberg R. T. 1966; A simplified method of sample preparations for determination of Tritium, Carbon-14 or Sulphur-35 in blood or tissue by liquid scintillation counting. Analytical Biochemistry 16:500–509
    [Google Scholar]
  17. Majumdar A. S., Dutta P., Dutta D., Ghose A. C. 1981; Antibacterial and antitoxin responses in the serum and milk of cholera patients. Infection and Immunity 32:1–8
    [Google Scholar]
  18. Manning P. A., Imbesi F., Haynes D. R. 1982; Cell envelope proteins in Vibrio cholerae. FEMS Microbiology Letters 14:159–166
    [Google Scholar]
  19. Manning P. A., Haynes D. R. 1984; A common-immunogenic Vibrio outer membrane protein. FEMS Microbiology Letters 24:297–302
    [Google Scholar]
  20. Markwell M. A. K., Haas S. M., Bieber L. L., Tolbert N. E. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87:206–210
    [Google Scholar]
  21. Richardson K., Parker C. D. 1985; Identification and characterization of Vibrio cholerae surface proteins by radioiodination. Infection and Immunity 48:87–93
    [Google Scholar]
  22. Sciortino C. V., Finkelstein R. A. 1983; Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo. Infection and Immunity 42:990–996
    [Google Scholar]
  23. Sears S. D., Richardson K., Young C., Parker C. D., Levine M. M. 1984; Evaluation of the human response to outer membrane proteins of Vibrio cholerae. Infection and Immunity 44:439–444
    [Google Scholar]
  24. Sharma D. P., Attridge S., Hackett J., Rowley D. 1987; Nonlipopo-lysaccharide protective antigens shared by classical and ElTor biotypes of Vibrio cholerae. Journal of Infectious Diseases 155:716–723
    [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
  26. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-29-1-33
Loading
/content/journal/jmm/10.1099/00222615-29-1-33
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error