1887

Abstract

SUMMARY

When the incubation period of primary isolation plates was extended to 48 h, mucoid strains of were found in specimens from various infected sites in patients who did not have cystic fibrosis. The 17 mucoid isolates were characterised in terms of mucoid type, pyocin type, and their sensitivity or resistance to seven -lactam and two aminoglycoside antibiotics. The carbohydrate, uronic acid (alginate) and protein content of the water-soluble extracellular material of 15 strains was determined. This material was fractionated by ion-exchange chromatography, and the presence of alginate confirmed by the chemical assay of uronic acids and their quantitation by gas-liquid chromatography. Uronic acids were absent from a nonmucoid revertant of one strain. The strains produced alginate with a high content of mannuronic acid and substituted with O-acetyl groups. By proton nuclear magnetic resonance (H-nmr) analysis the alginate from three strains was shown to lack polyguluronate blocks in its structure. These properties are also found in the alginate of mucoid strains from patients with cystic fibrosis.

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1989-03-01
2024-04-25
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References

  1. Buscher H. P., Casals-Stenzel J., Schauer R. 1974; New sialic acids. Identification of N-glycoloyl-O-acetylneuraminic acids and N-acetyl-O-glycoloylneuraminic acids by improved methods for detection of N-acyl and O-acyl groups and by gas-liquid chromatography. European Journal of Biochemistry 50:71–82
    [Google Scholar]
  2. Carlson D. M., Matthews L. W. 1966; Polyuronic acids produced by Pseudomonas aeruginosa. Biochemistry 5:2817–2822
    [Google Scholar]
  3. Chester I. R., Gray G. W., Wilkinson S. G. 1972; Further studies of the chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa. Biochemical Journal 126:395–407
    [Google Scholar]
  4. Clamp J. R., Dawson G., Hough L. 1967; The simultaneous estimation of 6-deoxy-l-galactose (l-fucose), d-mannose, d-galactose, 2-acetamido-2-deoxy-d-glucose (N-acetyl-d-glucosamine) and N-acetylneuraminic acid (sialic acid) in glycopeptides and glycoproteins. Biochimica et Biophysica Acta 148:342–349
    [Google Scholar]
  5. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Evans L. R., Linker A. 1973; Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa. Journal of Bacteriology 116:915–924
    [Google Scholar]
  7. Fyfe J. A. M., Govan J. R. W. 1980; Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. Journal of General Microbiology 119:443–450
    [Google Scholar]
  8. Gillies R. R., Govan J. R. W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. Journal of Pathology and Bacteriology 91:339–345
    [Google Scholar]
  9. Govan J. R. W. 1978; Pyocin typing of Pseudomonas aeruginosa. In Bergen T., Norris J. R. (eds) Methods in microbiology vol 10 Academic Press; London: pp 61–91
    [Google Scholar]
  10. Govan J. R. W., Fyfe J. A. M., Baker N. R. 1983; Heterogeneity and reduction in pulmonary clearance of mucoid Pseudomonas aeruginosa. Reviews of Infectious Diseases 5: Suppl 5: S874–S879
    [Google Scholar]
  11. Govan J. R. W., Harris G. S. 1986; Pseudomonas aeruginosa and cystic fibrosis: unusual bacterial adaptation and pathogenesis. Microbiological Sciences 3:302–308
    [Google Scholar]
  12. Grasdalen H., Larsen B., Smidsrod O. 1979; A p.m.r. study of the composition and sequence of uronate residues in alginates. Carbohydrate Research 68:23–31
    [Google Scholar]
  13. Haug A., Larsen B. 1962; Quantitative determination of the uronic acid composition of alginates. Acta Chimica Scandinavica 16:1908–1918
    [Google Scholar]
  14. Hugh R., Leifson E. 1953; The taxonomic significance of fermentation versus oxidative metabolism of carbohydrates by various gram-negative bacteria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  15. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine 44:301–307
    [Google Scholar]
  16. Knutson C. A., Jeanes A. 1968; Determination of the composition of uronic acid mixtures. Analytical Biochemistry 24:482–490
    [Google Scholar]
  17. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  18. Linker A., Jones R. S. 1966; A new polysaccharide resembling alginic acid isolated from pseudomonads. Journal of Biological Chemistry 241:3845–3851
    [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  20. Russell N. J., Gacesa P. 1988; Chemistry and biology of the alginate of mucoid strains of Pseudomonas aeruginosa in cystic fibrosis. Molecular Aspects of Medicine 10:1–91
    [Google Scholar]
  21. Sherbrock-Cox V., Russell N. J., Gacesa P. 1984; The purification and chemical characterisation of the alginate present in the extracellular material produced by mucoid strains of Pseudomonas aeruginosa. Carbohydrate Research 135:147–154
    [Google Scholar]
  22. Williams R. J., Govan J. R. W. 1973; Pyocine typing of mucoid strains of Pseudomonas aeruginosa isolated from children with cystic fibrosis. Journal of Medical Microbiology 6:409–412
    [Google Scholar]
  23. World Health Organization Technical Report Series No. 210 1961; Standardization of methods for conducting microbial sensitivity tests.
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