1887

Abstract

The mechanisms by which resident peritoneal macrophages, activated by lymphokines (LK) or recombinant γ-interferon (IFN), kill or were studied. Resident non-activated peritoneal macrophages killed (55·5%SD6·8%), but not . This killing was completely inhibited by superoxide dismutase (SOD), partially by dimethyl sulphoxide (DMSO), but not by catalase or azide. Killing correlated with a brisk lucigenin-dependent chemilumi-nescence(CL) response by macrophages interacting with . No enhanced luminol-dependent CL response was observed in this system. This suggests that is killed by resident macrophages a mechanism dependent on the presence of superoxide anion. By contrast, killing of by activated macrophages (49·0%SD5·9%) was not inhibited by SOD or DMSO, suggesting the induction of a non-oxidative candidacidal mechanism. was killed only by macrophages activated with IFN (52·0%SD3·7%) or LK (55·7%SD2·8%). Inhibition of killing by SOD was greater in IFN- than in LK-activated macrophages. Conversely, killing by LK-, but not IFN-, activated macrophages was significantly inhibited by catalase, DMSO or azide. The killing by LK-activated macrophages, and its inhibition by scavengers, correlated with the luminol-dependent CL response. The non-killing resident macrophages interacting with made lucigenin-dependent CL responses similar to those of activated macrophages. The mechanisms enabling killing of induced by activation appear to be different for LK and IFN, and appear to depend upon the myeloperoxidase systems and superoxide respectively.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-28-3-173
1989-03-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-28-3-173
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error