1887

Abstract

Summary

The intracellular and extracellular protease and elastase contents of were studied in relation to the age of the culture. The intracellular protease and elastase content of the organisms decreased as the culture grew older, whereas extracellular protease and elastase greatly increased 24 h after incubation commenced, suggesting that host tissue injury by infection may increase in proportion to the duration of tissue colonisation.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-28-2-109
1989-02-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jmm/28/2/medmicro-28-2-109.html?itemId=/content/journal/jmm/10.1099/00222615-28-2-109&mimeType=html&fmt=ahah

References

  1. Aduan R. P., Reynolds H. Y. 1979; The importance of cell-mediated responses to Pseudomonas infection. Doggett R. (ed) In Pseudomonas aeruginosa: clinical management of infection and current therapy Academic Press; New York: pp 135–156
    [Google Scholar]
  2. Banno Y., Yano K., Nozawa Y. 1982; Biochemical characterization of secreted proteases during growth in Tetrahymena pyrifor-mis WH-14: Comparison of extracellular with intracellular proteases. Journal of Protozoology 29:91–98
    [Google Scholar]
  3. Christensen G. D., Simpson W. A., Beachey E. H. 1985; Adhesion of bacteria to animal tissues: Complex mechanisms. In Savage D. C., Fletcher M. M. (eds) Bacterial adhesion: mechanisms and physiological significance Plenum Press; New York: pp 279–305
    [Google Scholar]
  4. Feigin R. D., Shearer W. T. 1975; Opportunistic infection in children. II In the compromised host. Journal of Pediatrics 87:677–694
    [Google Scholar]
  5. Johnson G. G., Morris J. M., Berk R. S. 1967; The extracellular protease from Pseudomonas aeruginosa exhibiting elastase activity. Canadian Journal of Microbiology 13:711–719
    [Google Scholar]
  6. Kanungo M. S. (ed) 1980; Biochemistry of ageing. Academic Press; New York: pp 79–128
    [Google Scholar]
  7. Kreger A. S., Gray L. D. 1978; Purification of Pseudomonas aeruginosa protease and microscopic characterization of pseudomonal protease-induced rabbit corneal damage. Infection and Immunity 19:630–648
    [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  9. Meinke G., Barum J., Rosenberg B., Berk R. 1970; In-vivo studies tease and elastase may play an important role in establishing these host and tissue tropisms. Partial purification and characterisation of these proteases are currently under investigation in our division. with the partially purified protease (elastase) from Pseudomonas aeruginosa. Infection and Immunity 2:583–589
    [Google Scholar]
  10. Morihara K., Tsuzuki H. 1977; Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients. Infection and Immunity 15:679–685
    [Google Scholar]
  11. Morihara K., Tsuzuki H., Oda K. 1979; Protease and elastase of Pseudomonas aeruginosa: inactivation of human plasma α 1-proteinase inhibitor. Infection and Immunity 24:188–193
    [Google Scholar]
  12. Rinderknecht H., Geokas M. C., Silverman P., Haverback B. J. 1968; A new ultrasensitive method for the determination of proteolytic activity. Clinica Chimica Acta 21:197–203
    [Google Scholar]
  13. Sabath L. D. ed) 1980; Pseudomonas aeruginosa: the organism, diseases it causes, and their treatment. H. Huber, Bern;
    [Google Scholar]
  14. Umeki S., Fukushima H., Nozawa Y. 1983; Variations in the activity of fatty acyl-CoA desaturases and electron-trans port components in Tetrahymena microsomes with temperature and age of culture. Journal of Thermal Biology 8:353–360
    [Google Scholar]
  15. Umeki S., Nozawa Y. 1983; Effects of sterol manipulation on microsomal desaturase activities in Tetrahymena: with regard to thermal acclimation. Biochemical and Biophysical Research Communications 113:96–101
    [Google Scholar]
  16. Umeki S. 1987; Factors influencing bacterial adhesion in the respiratory tract. The 60th International Symposium on Infectious Diseases Tokyo 1987
    [Google Scholar]
  17. Woods D. E., Straus D. C., Johanson W. G., Bass J. A. 1981; Role of salivary protease activity in adherence of gram-negative bacilli to mammalian buccal epithelial calls in vivo. Journal of Clinical Investigation 68:1435–1440
    [Google Scholar]
  18. Wretlind B., Wadstrom T. 1977; Purification and properties of a protease with elastase activity from Pseudomonas aerugi nosa. Journal of General Microbiology 103:319–327
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-28-2-109
Loading
/content/journal/jmm/10.1099/00222615-28-2-109
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error