A coagglutination test was developed for identifying suspected colonies of serotype O1 directly from primary isolation plates. Visible agglutination occurs when O1 antibody attached to cell-wall protein A of reacts with its homologous antigen. From 314 faecal samples from clinically suspected cases of cholera, 210 colonies from thiosulphate citrate bile salts sucrose (TCBS) agar and 222 colonies from taurocholate tellurite gelatin (TTG) agar were tested as suspect In each case 204 isolates were identified as O1 by conventional methods and also gave positive results for O1 in the coagglutination test; with one partial exception, no other colonies tested gave positive results. The coagglutination test is simple and inexpensive and provides a result 24 h earlier than conventional methods.


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