1887

Abstract

Summary

A coagglutination test was developed for identifying suspected colonies of serotype O1 directly from primary isolation plates. Visible agglutination occurs when O1 antibody attached to cell-wall protein A of reacts with its homologous antigen. From 314 faecal samples from clinically suspected cases of cholera, 210 colonies from thiosulphate citrate bile salts sucrose (TCBS) agar and 222 colonies from taurocholate tellurite gelatin (TTG) agar were tested as suspect In each case 204 isolates were identified as O1 by conventional methods and also gave positive results for O1 in the coagglutination test; with one partial exception, no other colonies tested gave positive results. The coagglutination test is simple and inexpensive and provides a result 24 h earlier than conventional methods.

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/content/journal/jmm/10.1099/00222615-28-1-39
1989-01-01
2024-04-25
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References

  1. Farmer J. J., Hickman-Brenner F. W., Kelly M. T. 1985; Vibrio. In Lennette E. H. (eds) Manual of clinical microbiology 4th edn American Society for Microbiology; Washington DC: pp 282–301
    [Google Scholar]
  2. Fung F. C., Tilton R. C. 1985; Detection of bacterial antigens by counterimmunoelectrophoresis, coagglutination, and latex agglutination. In Lennette E. H. (eds) Manual of clinical microbiology 4th edn American Society for Microbiology; Washington DC: pp 883–890
    [Google Scholar]
  3. Kobayashi T., Enomoto S., Sakazaki R., Kuwahara S. 1963; A new selective isolation medium for the Vibrio group; on a modified Nakanishi’s medium (TCBS agar medium). Japanese Journal of Bacteriology 18:387–392
    [Google Scholar]
  4. Kronvall G. 1973; A rapid slide-agglutination method for typing pneumococci by means of specific antibody absorbed to protein A-containing staphylococci. Journal of Medical Microbiology 6:187–190
    [Google Scholar]
  5. Monsur K. A. 1961; A highly selective gelatin-taurocholate-tellurite medium for isolation of Vibrio cholerae. Transactions of the Royal Society of Tropical Medicine and Hygiene 55:440–442
    [Google Scholar]
  6. Morris G. K., Merson M. H., Huq I., Kibrya A. K. M. G., Black R. 1979; Comparison of four plating media for isolating Vibrio cholerae. Journal of Clinical Microbiology 9:79–83
    [Google Scholar]
  7. Rahman M., Sack D. A., Mahmood S., Hossain A. 1987; Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures. Journal of Clinical Microbiology 25:2204–2206
    [Google Scholar]
  8. Samadi A.R. 1983; Classical Vibrio cholerae biotype displaces El Tor in Bangladesh. Lancet 1:805–807
    [Google Scholar]
  9. Sivadasan K., Kurien B., John T. J. 1984; Rapid diagnosis of typhoid fever by antigen detection. Ixincet 1:134–135
    [Google Scholar]
  10. Thirumoorthi M. C., Dajani A. S. 1979; Comparison of staphylococcal coagglutination, latex agglutination and counterimmunoelectrophoresis for bacterial antigen detection. Journal of Clinical Microbiology 9:28–32
    [Google Scholar]
  11. World Health Organization 1974; Guidelines for the laboratory diagnosis of cholera. World Health Organization; Geneva:
    [Google Scholar]
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