1887

Abstract

Summary

Following natural or experimental primary infection, herpes simplex virus (HSV) becomes latent in sensory ganglia. Reactivation of latent virus may lead to recurrent disease. If HSV DNA remains stable during primary, recurrent and latent infections, that stability would enable us to trace the transmission of HSV from one individual to another. We inoculated mice in the ear pinna with HSV and collected virus at various intervals during primary infection. In mice surviving primary infections, recurrent disease was induced from which virus was isolated. Virus was also recovered from explanted dorsal root ganglia. Virus isolates were characterised by restriction endonuclease digestion and compared with the original inoculate(s). The data indicate that in all cases except two, the isolates from primary and recurrent infections remained identical to the original inoculates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-27-4-291
1988-12-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/27/4/medmicro-27-4-291.html?itemId=/content/journal/jmm/10.1099/00222615-27-4-291&mimeType=html&fmt=ahah

References

  1. Blyth W. A., Hill T. J. 1984; Establishment, maintenance, and control of herpes simplex virus (HSV) latency. In Rouse B. T., Lopez C. (eds) Immunobiology of herpes simplex virus infection CRC Press; Boca Raton: pp 9–32
    [Google Scholar]
  2. Buchman T. G., Roizman B., Nahmias A. J. 1979; Demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral DNA. Journal of Infectious Diseases 140:295–304
    [Google Scholar]
  3. Centifanto-Fitzgerald Y. M., Vamell E. D., Kaufman H. E. 1982; Initial herpes simplex virus type 1 infection prevents ganglionic superinfection by other strains. Infection and Immunity 35:1125–1132
    [Google Scholar]
  4. Embil J. A., Faulkner R. S. 1964; Human diploid cell strains and their susceptibility to viruses. Canadian Journal of Public Health 55:111–116
    [Google Scholar]
  5. Embil J. A., Manuel F. R., McFarlane E. S. 1981; Concurrent oral and genital infection with an identical strain of herpes simplex virus type 1: restriction endonuclease analysis. Sexually Transmitted Diseases 8:70–72
    [Google Scholar]
  6. Hill T. J., Blyth W. A., Harbour D. A. 1978; Trauma to the skin causes recurrence of herpes simplex in the mouse. Journal of General Virology 39:21–28
    [Google Scholar]
  7. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  8. Hirt B. 1967; Selective extractions of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  9. Kit S. 1983; Sequential genital infections by herpes simplex viruses types 1 and 2: restriction nuclease analyses of viruses from recurrent infections. Sexually Transmitted Diseases 10:67–71
    [Google Scholar]
  10. Klein R. J. 1982; The pathogenesis of acute, latent and recurrent herpes simplex virus infections: brief review. Archives of Virology 72:143–168
    [Google Scholar]
  11. Klein R. J. 1985; Initiation and maintenance of latent herpes simplex virus infections: the paradox of perpetual immobility and continuous movement. Reviews of Infectious Diseases 7:21–30
    [Google Scholar]
  12. McFarlane E. S., James H. 1984; Characterisation of Herpes simplex viral DNA using 3H-thymidine and restriction enzyme digestion analysis. Medical Laboratory Sciences 41:73–75
    [Google Scholar]
  13. McKendall R. R. 1977; Efficacy of herpes simplex virus type 1 immunisation in protecting against acute and latent infection by herpes simplex virus type 2 in mice. Infection and Immunity 16:717–719
    [Google Scholar]
  14. Morse L. S., Buchman T. G., Roizman B., Schaffer P. A. 1977; Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 x HSV-2) recombinants. Journal of Virology 24:231–248
    [Google Scholar]
  15. Roizman B., Buchman T. 1979; The molecular epidemiology of herpes simplex viruses. Hospital Practice 14:195–104
    [Google Scholar]
  16. Roizman B., Tognon M. 1982; Restriction enzyme analysis of herpesvirus DNA: stability of restriction endonuclease patterns. Lancet 1:677
    [Google Scholar]
  17. Smith I. W., Maitland N. J., Peutherer J. F., Robertson D. H. H. 1981; Restriction enzyme analysis of herpesvirus-2 DNA. Lancet 2:14–24
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-27-4-291
Loading
/content/journal/jmm/10.1099/00222615-27-4-291
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error