Transferable or mobilisable antibiotic resistance in types 1, 2, 3, 4, 6 and 7 isolated in Ethiopia during 1974–85 Free

Abstract

Summary

A total of 199 isolates resistant to one or more antibiotics and belonging to types 1, 2, 3, 4, 6 and 7 was examined by one-step broth mating with K12 and, if non-conjugative, additionally by triparental crosses with the conjugative plasmids X and ◿. Of the type 1 (Shiga's bacillus) strains, 96% harboured conjugative plasmids. During 1974-79, isolates of Shiga's bacillus carried conjugative plasmids coding for ACSSuT (ampicillin, chloramphenicol, streptomycin, sulphonamide, tetracycline) resistance that transferred at low frequencies (>10). After 1980, however, about 50% of isolates of Shiga's bacillus with this resistance (R)-type carried conjugative plasmids that transferred at high frequencies (10°−10) and that expressed the ACT determinant only. The introduction of a new clone of Shiga's bacillus into Ethiopia in 1980 is suspected. Conjugative plasmids coding for SSuT resistance were detected in types 2, 3, and 4. Non-conjugative SSu determinants in type 3 were mobilised by conjugative plasmids X and ◿. R-type CSSuT in strains of types 2 and 7, and R-type ACST in type-3 strains were neither transferable nor mobilisable and are probably determined chromosomally.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-27-4-285
1988-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/27/4/medmicro-27-4-285.html?itemId=/content/journal/jmm/10.1099/00222615-27-4-285&mimeType=html&fmt=ahah

References

  1. Akiba T., Koyama K., Ishiki Y., Kimura S., Fukushima T. 1960; On the mechanism of the development of multiple-drug-resistant clones of Shigella. Japanese Journal of Microbiology 4:219–227
    [Google Scholar]
  2. Anderson E. S., Threlfall E. J. 1974; The characterization of plasmids in the Enterobacteria. Journal of Hygiene 72:471–487
    [Google Scholar]
  3. Bauer A. W., Kirby W. M. M., Sherris J. C., Turch A. 1966; Antibiotic susceptibility testing by standardized single disk method. American Journal of Clinical Pathology 45:493–496
    [Google Scholar]
  4. Crosa J. H., Olarte J., Mata L. J., Luttrop L. K., Penaranda M. E. 1977; Characterization of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae type 1 isolated from epidemics. Antimicrobial Agents and Chemotherapy 11:553–558
    [Google Scholar]
  5. Ebright J. R., Moore E. C., Sanborn W. R., Schaberb D., Kyle J., Ishida K. 1984; Epidemic Shiga bacillus dysentery in Central Africa. American Journal of Tropical Medicine and Hygiene 33:1192–1197
    [Google Scholar]
  6. Frost J. A., Rowe B., Vandepitte J., Threlfall E. J. 1981; Plasmid characterisation in the investigation of an epidemic caused by multiply resistant Shigella dysenteriae type 1 in Central Africa. Lancet 2:1074–1076
    [Google Scholar]
  7. Frost J. A., Rowe B. 1983; Plasmid determined antibiotic resistance in Shigella flexneri isolated in England and Wales between 1974 and 1978. Journal of Hygiene 90:27–32
    [Google Scholar]
  8. Frost J. A., Willshaw G. A., Barclay E. A., Rowe B., Lemmens P., Vandepitte J. 1985; Plasmid characterization of drug resistant Shigella dysenteriae type 1 from an epidemic in Central Africa. Journal of Hygiene 94:163–172
    [Google Scholar]
  9. Gebre-Yohannes A., Drasar B. S. 1987; Shigella dysenteriae and S. flexneri: serotype prevalence and seasonal distribution in Addis Ababa, Ethiopia (1974–85). Ethiopian Journal of Health Development 2:51–58
    [Google Scholar]
  10. Gebre-Yohannes A., Habte-Gabr E. 1984a; Shigellosis in Ethiopia I. Prevalent Shigella serogroups and serotypes. Journal of Diarrohoeal Disease Research 2:79–82
    [Google Scholar]
  11. Gebre-Yohannes A., Habte-Gabr E. 1984b; Shigellosis in Ethiopia. II: Patterns of drug resistance in Shigella serotypes. Journal of Diarrhoeal Diseases Research 2:212–216
    [Google Scholar]
  12. Gebre-Yohannes A., Limenih Y. 1980; Multiple drug resistance within Shigella serogroups. Ethiopian Medical Journal 18:7–14
    [Google Scholar]
  13. Gedebou M., Tassew A. 1979; Antibiotic susceptibility patterns and R factor among Salmonella and Shigella isolates. Ethiopian Medical Journal 17:99–100 (abstract)
    [Google Scholar]
  14. Gedebou M., Tassew A. 1982; Shigella species from Addis Ababa: frequency of isolates and in-vitro drug sensitivity. Journal of Hygiene 88:47–55
    [Google Scholar]
  15. Howe T. G. B. 1982; Genetic modification of the organism and antibiotic resistance. In Stuart-Harris C. H., Harris D. M. (eds) The control of antibiotic resistant bacteria Academic Press; London: pp 97–117
    [Google Scholar]
  16. Macaden R., Gokul B. N., Pereira P., Bhat P. 1980; Bacillary dysentery due to multidrug-resistant Shigella dysenteriae type 1. Indian Journal of Medical Research 71:178–185
    [Google Scholar]
  17. Mata L. J., Gangarosa E. J., Caceres A., Perera D. R., Mejicanos M. L. 1970; Epidemic Shiga bacillus dysentery in Central America. I. Etiologic investigations in Guatemala, 1969. Journal of Infectious Diseases 122:170–180
    [Google Scholar]
  18. Ochiai K., Yamanaka T., Kimura K., Sawada O. 1959; Studies on the inheritance of drug resistance between Shigella strains and Escherichia coli strains. Nippon-Iji Shimpo 1861:34–46 (in Japanese)
    [Google Scholar]
  19. Olarte J., Filloy L., Galindo E. 1976; Resistance of Shigella dysenteriae type 1 to ampicillin and other antimicrobial agents: strains isolated during a dysentery outbreak in a hospital in Mexico City. Journal of Infectious Diseases 133:572–575
    [Google Scholar]
  20. Steers E., Foltz E. L., Graves B. S. 1959; An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics. Antibiotics and Chemotherapy (Washington) 9:307–311
    [Google Scholar]
  21. Suzuki S., Nakazawa S., Ushida T. 1956; Yearly changes of drug resistance of Shigella strains from 1951. Chemotherapy 4:336–338 (in Japanese)
    [Google Scholar]
  22. WHO Scientific Working Group 1983; Antimicrobial resistance. Bulletin of the World Health Organization 61:383–394
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-27-4-285
Loading
/content/journal/jmm/10.1099/00222615-27-4-285
Loading

Data & Media loading...

Most cited Most Cited RSS feed