1887

Abstract

Summary

Molecular variants of the serotype-specific plasmid (SSP) of (pSLT) were recognised in clinical and veterinary isolates by restriction enzyme fingerprinting. Three had undergone minor DNA rearrangements, whereas two had acquired resistance determinants to a wide range of antimicrobial agents including gentamicin, trimethoprim, tetracycline, streptomycin, ampicillin (Ap) and kanamycin (Km). One of the latter was the result of co-integrate formation with an IncX, conjugative R-plasmid that specified ApKm resistance. The co-integrate plasmid (pOG669) was incompatible with, and displaced, pSLT and its molecular variants. The restriction fingerprints of SSPs of and were compared with pSLT. All were related at the 35% level on the basis of a Dice coefficient of similarity. The SSPs of and were incompatible with the co-integrate plasmid pOG669. Whereas in this resulted from incompatibility with the pSLT component (the SSP was compatible with the IncX component), the converse was found with .

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1988-12-01
2024-10-09
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References

  1. Anderson E. S., Smith H. R. 1972; Fertility inhibition in strains of Salmonella typhimurium. Molecular and General Genetics 118:79–84
    [Google Scholar]
  2. Baird G. D., Manning E. J., Jones P. W. 1985; Evidence for related virulence sequences in plasmids of Salmonella dublin and Salmonella typhimurium. Journal of General Microbiology 131:1815–1823
    [Google Scholar]
  3. Bennett P. M., Heritage J., Comanducci A., Dodd H. M. 1986; Evolution of R-plasmids by replicon fusion. Journal of Antimicrobial Chemotherapy 18: Suppl C 103–111
    [Google Scholar]
  4. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  5. Brown D. J., Munro D. S., Platt D. J. 1986; Recognition of the cryptic plasmid, pSLT by restriction fingerprinting and a study of its incidence in Scottish Salmonella isolates. Journal of Hygiene 97:193–197
    [Google Scholar]
  6. Casalino M., Comanducci A., Nicoletti M., Maimone F. 1984; Stability of plasmid content in Salmonella wein in late phases of epidemic history. Antimicrobial Agents and Chemotherapy 25:499–501
    [Google Scholar]
  7. Dice L. R. 1945; Measures of the amount of ecological association between species. Ecology 26:297–302
    [Google Scholar]
  8. Farrar W. E. 1983; Molecular analysis of plasmids in epidemiologic investigation. Journal of Infectious Diseases 148:1–6
    [Google Scholar]
  9. Hawkey P. M. 1987; Molecular methods for the investigation of bacterial cross-infection. Journal of Hospital Infection 9:211–218
    [Google Scholar]
  10. Helmuth R., Stephan R., Bunge C., Hoog B., Steinbeck A., Bulling E. 1985; Epidemiology of virulence-associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes. Infection and Immunity 48:175–182
    [Google Scholar]
  11. Jones G. W., Rabert D. K., Svinarich D. M., Whitfield H. J. 1982; Association of adhesive, invasive and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids. Infection and Immunity 38:476–486
    [Google Scholar]
  12. McConnell M. M., Smith H. R., Leonardopoulos J., Anderson E. S. 1979; The value of plasmid studies in the epidemiology of infections due to drug-resistant Salmonella wein. Journal of Infectious Diseases 139:178–190
    [Google Scholar]
  13. Nakamura M., Sato S., Ohya T., Suzuki S., Ikeda S. 1985; Possible relationship of 36–megadalton Salmonella enteritidis plasmid to virulence in mice. Infection and Immunity 41:831–833
    [Google Scholar]
  14. Platt D. J. 1987; Studies with Scottish salmonellae. Infections in Focus 1:9–10
    [Google Scholar]
  15. Platt D. J., Chesham J. S., Brown D. J., Kraft C. A., Taggart J. 1986; Restriction enzyme fingerprinting of enterobacterial plasmids:a simple strategy with wide application. Journal of Hygiene 97:205–210
    [Google Scholar]
  16. Platt D. J., Sommerville J. S. 1981; A simple method for the detection of resistance plasmids in Serratia species by transfer to members of the genus Enterobacter. Journal of Antimicrobial Chemotherapy 8:145–152
    [Google Scholar]
  17. Platt D. J., Sommerville J. S., Gribben J. 1984; Sequential acquisition of R-plasmids in vivo by Salmonella typhimurium. Journal of Antimicrobial Chemotherapy 13:65–69
    [Google Scholar]
  18. Platt D. J., Taggart J. 1987; Molecular epidemiology: determination of plasmid sizes. Focus 9:13
    [Google Scholar]
  19. Popoff M. Y., Miras I., Coynault C., Lasselin C., Pardon P. 1984; Molecular relationships between virulence plasmids of Salmonella serotypes typhimurium and dublin and large plasmids of other Salmonella serotypes. Annales de Microbiologie (Paris) 135:389–398
    [Google Scholar]
  20. Prado D., Murray B. E., Cleary T. G., Pickering L. K. 1987; Limitations of using the plasmid pattern as an epidemiological tool for clinical isolates of Shigella sonnei. Journal of Infectious Diseases 155:314–316
    [Google Scholar]
  21. Robinson M. K., Bennett P. M., Falkow S., Dodd H. M. 1980; Isolation of a temperature-sensitive derivative of RP1. Plasmid 3:343–347
    [Google Scholar]
  22. Terakado N., Sekizaki T., Hashimoto K., Naitoh S. 1983; Correlation between the presence of a fifty-megadalton plasmid in Salmonella dublin and virulence for mice. Infection and Immunity 41:443–444
    [Google Scholar]
  23. Willshaw G. A., Smith H. R., Anderson E. S. 1978; Molecular studies of F1me resistance plasmids, particularly in epidemic Salmonella typhimurium. Molecular and General Genetics 159:111–116
    [Google Scholar]
  24. Zinder N. D., Lederberg J. 1952; Genetic exchange in Salmonella. Journal of Bacteriology 64:679–699
    [Google Scholar]
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