1887

Abstract

Summary

We have generated monoclonal antibodies (MABs) to staphylococcal enterotoxin B (SEB) in BALB/c mice. Five out of 20 clones which produce anti-SEB MABs have been characterised. Among them, three produce IgG/k, one produces IgM/, and one apparently produces both IgG/ and IgM/ MABs. The anti-SEB titres of ascites fluids range from 3200 to >819200 by ELISA. All of the MABs analysed thus far neutralise the mitogenic response of BALB/c splenocytes to a suboptimal dose of SEB. Also, the induction of suppressor cells by SEB is reversed by pre-incubating SEB with these MABs. Limited digestion with chymotrypsin, trypsin or V8 protease yields peptide fragments which have been tested by Western-blot analysis. MABs 1FD7 and 2GD9 are specific for the carboxy-terminal end of SEB, and have a similar, but not identical, binding epitope. MABs 2DA3 and 2HA10 bind to intact SEB but not to cleaved products, and are probably specific for antigenic determinants altered by the cleavage or by the denaturing conditions of the electrophoresis, or by both.

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1988-12-01
2024-03-29
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References

  1. Adrion R. F. 1982; Immunoassay design equation. Clinical Chemistry 28:717
    [Google Scholar]
  2. Bergdoll M. S. 1979; Staphylococcal intoxications. In Riemann H., Bryan F. L. (eds) Food-borne infections and intoxications 2nd edn Academic Press; New York: pp 443–494
    [Google Scholar]
  3. Bergdoll M. S. 1983; Enterotoxins. In Easmon C. S. F., Adlam C. (eds) Staphylococci and staphylococcal infections vol 2 Chapter 16: The organism in vivo and in vitro. Academic Press; New York: pp 559–598
    [Google Scholar]
  4. Cunningham A. J., Szenberg A. 1968; Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology 14:599–600
    [Google Scholar]
  5. Dalidowicz J. E., Silverman S. J., Schantz E. J., Stefanye D., Spero L. 1966; Chemical and biological properties of reduced and alkylated staphylococcal enterotoxin B. Biochemistry 5:2375–2381
    [Google Scholar]
  6. Donnelly R. P., Rogers T. J. 1982; Immunosuppression induced by staphylococcal enterotoxin B. Cellular Immunology 72:166–111
    [Google Scholar]
  7. Huang I. Y., Bergdoll M. S. 1970; The primary structure of staphylococcal enterotoxin B. III. The cyanogen bromide peptides of reduced and aminoethylated enterotoxin B. and the complete amino acid sequence. Journal of Biological Chemistry 245:3518–3525
    [Google Scholar]
  8. Kappler J. W. 1974; A micro-technique for hemolytic plaque assays. Journal of Immunology 112:1271–1274
    [Google Scholar]
  9. Lin Y. S., Rogers T. J. 1986; Inhibition of antibody production from the plasmacytoma cell line MOPC-315 by staphylococcal enterotoxin B-induced T-suppressor cells. Cellular Immunology 102:299–306
    [Google Scholar]
  10. Middlebrook J. L., Spero L., Argos P. 1980; The secondary structure of staphylococcal enterotoxin A. B and C. Biochimica et Biophysica Acta 621:233–240
    [Google Scholar]
  11. Miller B. A., Reiser R. F., Bergdoll M. S. 1978; Detection of staphylococcal enterotoxins A. B, C, D, and E in foods by radio-immunoassay, using staphylococcal cells containing protein A as immunoadsorbent. Applied and Environmental Microbiology 36:421–426
    [Google Scholar]
  12. Peavy D. L., Adler W. H., Smith R. T. 1970; The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. Journal of Immunology 105:1453–1458
    [Google Scholar]
  13. Ranelli D. M., Jones C. L., Johns M. B., Mussey G. J., Khan S. A. 1985; Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus. Proceedings of the National Academy of Sciences of the USA 82:5850–5854
    [Google Scholar]
  14. Rivas V., Rogers T. J. 1983; Studies on the cellular nature of Candida albicans—induced suppression. Journal of Immunology 130:376–379
    [Google Scholar]
  15. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. 1965; Purification of staphylococcal enterotoxin B. Biochemistry 4:1011–1016
    [Google Scholar]
  16. Spero L., Griffin B. Y., Middlebrook J. L., Metzger J. F. 1976; Effect of single and double peptide bond scission by trypsin on the structure and activity of staphylococcal enterotoxin C. Journal of Biological Chemistry 251:5580–5588
    [Google Scholar]
  17. Spero L., Metzger J. F., Warren J. R., Griffin B. Y. 1975; Biological activity and complementation of the two peptides of staphylococcal enterotoxin B formed by limited tryptic hydrolysis. Journal of Biological Chemistry 250:5026–5032
    [Google Scholar]
  18. Spero L., Warren J. R., Metzger J. F. 1973; Effect of single peptide bond scission by trypsin on the structure and activity of staphylococcal enterotoxin B. Journal of Biological Chemistry 248:7289–7294
    [Google Scholar]
  19. Strockbine N. A., Largen M. T., Buckley H. R. 1984; Production and characterization of three monoclonal antibodies to Candida albicans proteins. Infection and Immunity 43:1012–1018
    [Google Scholar]
  20. Thompson N. E., Ketterhagen M. J., Bergdoll M. S. 1984; Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments. Infection and Immunity 45:281–285
    [Google Scholar]
  21. Tittle T. V., Rittenberg M. B. 1978; Expression of IgG memory response in vitro to thymus-dependent and thymus-independent antigens. Cellular Immunology 35:180–190
    [Google Scholar]
  22. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
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