1887

Abstract

Summary

A set of 24 monoclonal antibodies (MABs) was produced against an outer envelope preparation from serovar . The MABs reacted in enzyme immunoassay with species-specific determinants of an antigen in the leptospiral outer envelope (OE) of pathogenic but not of saprophytic species of . The MABs did not agglutinate whole leptospires, nor could they opsonise homologous leptospires for phagocytosis by mouse macrophages or protect new-born guinea-pigs against lethal infection. The MABs reacted by Western blotting with a 35 × 10-mol-wt band in OE separated on SDS-polyacrylamide gels, and also reacted with other bands to a lesser extent. The determinants to which the MABs were directed were localised in the leptospiral OE by immunogold labelling techniques.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-27-1-51
1988-09-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/27/1/medmicro-27-1-51.html?itemId=/content/journal/jmm/10.1099/00222615-27-1-51&mimeType=html&fmt=ahah

References

  1. Adler B., Faine S. 1976; Susceptibility of mice treated with cyclophosphamide to lethal infection with Leptospira interrogans serovar pomona. Infection and Immunity 14:703–708
    [Google Scholar]
  2. Adler B., Faine S. 1978a; The antibodies involved in the human immune response to leptospiral infection. Journal of Medical Microbiology 11:387–400
    [Google Scholar]
  3. Adler B., Faine S. 1978b; Serological and protective-antibody responses of rabbits to leptospiral antigens. Journal of Medical Microbiology 11:401–409
    [Google Scholar]
  4. Adler B., Faine S., Yanagawa R. 1980; Comparative studies on two antigens (F4 and TM) extracted from leptospires. Journal of Clinical Microbiology 12:7–9
    [Google Scholar]
  5. Adler B., Faine S. 1983a; A pomona serogroup-specific, agglutinating antigen in Leptospira, identified by monoclonal antibodies. Pathology 15:247–250
    [Google Scholar]
  6. Adler B., Faine S. 1983b; Species- and genus-specific antigens in Leptospira, revealed by monoclonal antibodies and enzyme immunoassay. Zentralblatt fur Bakteriologie, Parasiten-kunde, Infektionskrankheiten und Hygiene, Abteilung I, Originale Reihe A 255:317–322
    [Google Scholar]
  7. Anderson D. L., Johnson R. C. 1968; Electron microscopy of immune disruption of leptospires: Action of complement and lysozyme. Journal of Bacteriology 95:2293–2309
    [Google Scholar]
  8. Auran N. E., Johnson R. C., Ritzi D. M. 1972; Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters. Infection and Immunity 5:968–975
    [Google Scholar]
  9. Auran N. E., Johnson R. C., Alexander A. D. 1975; Chemical and serological activity of leptospiral outer envelope. Leptospira and other Spirochaeta Proceedings of the National Symposium on Leptospirosis; Bucharest, Romania: pp 277–286
    [Google Scholar]
  10. Barbour A. G., Tessier S. L., Hayes S. F. 1984; Variation in a major surface protein of Lyme disease spirochetes. Infection and Immunity 45:94–100
    [Google Scholar]
  11. Bey R. F., Auran N. E., Johnson R. C. 1974; Immunogenicity of whole cell and outer envelope leptospiral vaccines in hamsters. Infection and Immunity 10:1051–1056
    [Google Scholar]
  12. Bey R. F., Johnson R. C. 1978a; Humoral immune response of dogs vaccinated with leptospiral pentavalent outer envelope and whole culture vaccines. American Journal of Veterinary Research 39:831–836
    [Google Scholar]
  13. Bey R. F., Johnson R. C. 1978b; Humoral immune responses of cattle vaccinated with leptospiral pentavalent outer envelope and whole culture vaccines. American Journal of Veterinary Research 39:1109–1114
    [Google Scholar]
  14. Bey R. F., Johnson R. C. 1982; Immunogenicity and humoral and cell-mediated immune responses to leptospiral whole cell, outer envelope and protoplasmic cylinder vaccines in hamsters and dogs. American Journal of Veterinary Research 43:835–840
    [Google Scholar]
  15. Broughton E. S., Scarnell T. 1985; Prevention of renal carriage of leptospirosis in dogs at vaccination. Veterinary Record 117:307–311
    [Google Scholar]
  16. Faine S., der Hoeden J. 1964; Virulence linked colonial and morphological variation in Leptospira. Journal of Bacteriology 88:1493–1496
    [Google Scholar]
  17. Farrelly H. E., Adler B., Faine S. 1987; Opsonic monoclonal antibodies against lipopolysaccharide antigens of Leptospira interrogans serovar hardjo. Journal of Medical Microbiology 23:1–7
    [Google Scholar]
  18. Glosser J. W., Johnson R. C., Sulzer C. R., Auran N. E. 1974; Immunogenic properties of a leptospiral outer envelope bacterin in hamsters and foxes. American Journal of Veterinary Research 35:681–684
    [Google Scholar]
  19. Hancock R. E. W., Wieczorek A. A., Mutharia L. M., Poole K. 1982; Monoclonal antibodies against Pseudomonas aeruginosa outer membrane antigens: Isolation and characterization. Infection and Immunity 37:166–171
    [Google Scholar]
  20. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  21. Hitchcock P. J., Brown T. M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. Journal of Bacteriology 154:269–277
    [Google Scholar]
  22. Johnson R. C. 1977; The Spirochetes. Annual Review of Microbiology 31:89–106
    [Google Scholar]
  23. Johnson R. C., Faine S. 1984 In Bergey’s Manual of systematic bacteriology vol 1 Williams and Wilkins; Baltimore, MD: pp 62–67
    [Google Scholar]
  24. Jost B. H., Farrelly H. E., Adler B., Faine S. 1987; Identification of Leptospira by species-specific monoclonal antibody. Tropical and Geographical Medicine 39:S512–513
    [Google Scholar]
  25. Jost B. H., Adler B., Vinh T., Faine S. 1986; A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea-pigs against leptospirosis. Journal of Medical Microbiology 22:269–275
    [Google Scholar]
  26. Morrissey J. H. 1981; Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  27. Nunes-Edwards P. L., Thiermann A. B., Bassford P. J., Stamm L. V. 1985; Identification and characterization of the protein antigens of Leptospira interrogans serovar hardjo. Infection and Immunity 48:492–497
    [Google Scholar]
  28. Ono E., Naiki M., Yanagawa R. 1984; Isolation of an antigenic oligosaccharide fraction from Leptospira interrogans serovar canicola with a monoclonal antibody. Journal of General Microbiology 130:1429–1435
    [Google Scholar]
  29. Poxton I. R., Bell G. T., Barclay G. R. 1985; The association on SDS-polyacrylamide gels of lipopolysaccharide and outer membrane proteins of Pseudomonas aeruginosa as revealed by monoclonal antibodies and Western blotting. FEMS Microbiology Letters 27:247–251
    [Google Scholar]
  30. Sawada S., Suzuki M., Kawamura T., Fujinaga S., Masuho Y., Tomibe K. 1984; Protection against infection with Pseudomonas aeruginosa by passive transfer of monoclonal antibodies to lipopolysaccharide and outer membrane proteins. Journal of Infectious Diseases 150:570–576
    [Google Scholar]
  31. Shinagawa M., Yanagawa R. 1972; Isolation and characterization of a leptospiral type-specific antigen. Infection and Immunity 5:12–19
    [Google Scholar]
  32. Takashima I., Yanagawa R. 1975; Immunizing effects of structural components of Abteilung I. Originate Reihe A 233:93–98
    [Google Scholar]
  33. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
  34. Tu V., Adler B., Faine S. 1982; The role of macrophages in the protection of mice against leptospirosis: in vitro and in vivo studies. Pathology 14:463–468
    [Google Scholar]
  35. Vinh T., Adler B., Faine S. 1986; Ultrastructure and chemical composition of lipopolysaccharide extracted from Leptospira interrogans serovar copenhageni. Journal of General Microbiology 132:103–109
    [Google Scholar]
  36. Woodward M. P., Young W. W., Bloodgood R. A. 1985; Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. Journal of Immunological Methods 78:143–153
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-27-1-51
Loading
/content/journal/jmm/10.1099/00222615-27-1-51
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error