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1887

Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 26, Issue 4

Differentiation of phase I and variant strains of on Congo red media No Access

Abstract

SUMMARY

The addition of Congo red, Trypan blue or haemin to the growth medium allowed the differentiation of phase-I and variant strains of . Phase-I strains produced red (CR), blue or dark brown colonies on a modified cyclodextrin solid medium containing Congo red, Trypan blue or haemin, respectively, whereas variant (Vir and phase IV) strains grew as pale (CR) colonies. Spontaneous CR variants were isolated and characterised and had a phenotype like that of Vir or phenotypically modulated, C-mode strains in that they did not produce the haemolysin, haemagglutinin(s), histamine-sensitising factor (pertussis toxin), heat-labile toxin and two major envelope polypeptides associated with phase-I strains. Two such variants had reduced virulence for mice. CR strains, when grown on a high nicotinic acid medium to induce modulation, gave CR colonies. Thus the CR phenotype is a characteristic of phase-I and its expression appears to be controlled in a manner similar to that of other phase I-related factors. CR variants of and were also deficient in these factors. Four isolates of were CR.

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© J. MED. MICROBIOL. 1988
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1988-08-01
2025-04-25
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References

  1. Berkhoff, H A., Vinal A. C. 1986; Congo red medium to distinguish between invasive and non-invasive Escherichia coli pathogenic for poultry. Avian Diseases 30:117–121
     [Google Scholar]
  2. Brownlie R. M., Parton R., Coote J. G. 1985; The effect of growth conditions on adenylate cyclase activity and virulence-related properties of Bordetella pertussis. Journal of General Microbiology 131:17–25
     [Google Scholar]
  3. Carter E. J., Preston N. W. 1981; Pulmonary pertussis infection in the mouse: no solution to an old problem. Journal of Infection 3:86–88
     [Google Scholar]
  4. Coote J. G., Brownlie R. M. 1988; Genetics of virulence of Bordetella pertussis. Wardlaw A. C., Parton R. (eds) Pathogenesis and immunity in pertussis John Wiley and Sons Ltd; Chichester: in press
    [Google Scholar]
  5. Fish F., Navon Y., Goldman S. 1987; Hydrophobic adherence and phase variation in Bordetella pertussis. Medical Microbiology and Immunology 176:37–46
     [Google Scholar]
  6. Daskaleros P. A., Payne S. M. 1987; Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model. Infection and Immunity 55:1393–1398
     [Google Scholar]
  7. Goldman S., Hanski E., Fish F. 1984; Spontaneous phase variation in Bordetella pertussis is a multistep non-random process. The EMBO Journal 3:1353–1356
     [Google Scholar]
  8. Griffiths E. 1983; Adaptation and multiplication of bacteria in host tissues. Philosophical Transactions of the Royal Society of London 303: series B 85–96
     [Google Scholar]
  9. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. 1983; Heptakis (2, 6-O-dimethyl) B-cyclodextrin: a novel growth stimulant for Bordetella pertussis phase I. Journal of Clinical Microbiology 17:781–786
     [Google Scholar]
  10. Ishiguro E. E., Ainsworth T., Trust T. J., Kay W. W. 1985; Congo red agar, a differential medium for Aeromonas salmonicida detects the presence of the cell surface protein array involved in virulence. Journal of Bacteriology 164:1233–1237
     [Google Scholar]
  11. Kay W. W., Phipps B. M., Ishiguro E. E., Trust T. J. 1985; Porphyrin binding by the surface array virulence protein of Aeromonas salmonicida. Journal of Bacteriology 164:1332–1336
     [Google Scholar]
  12. Kersters K. 1984; Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. International Journal of Systematic Bacteriology 34:56–70
     [Google Scholar]
  13. Lacey B. W. 1960; Antigenic modulation of Bordetella pertussis. Journal of Hygiene 58:57–93
     [Google Scholar]
  14. Leslie P. H., Gardner A. D. 1931; The phases of Haemophilus pertussis. Journal of Hygiene 31:423–434
     [Google Scholar]
  15. Maurelli A. T., Blackmon B., Curtiss R. 1984; Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid. Infection and Immunity 43:397–401
     [Google Scholar]
  16. Parton R. 1986; Effect of anti-inflammatory agents on the haemorrhagic response of mouse skin to Bordetella pertussis heat-labile toxin. Journal of Medical Microbiology 21:265–270
     [Google Scholar]
  17. Parton R., Wardlaw A. C. 1975; Cell-envelope proteins of Bordetella pertussis. Journal of Medical Microbiology 8:47–57
     [Google Scholar]
  18. Payne S. M., Finkelstein R. A. 1977; Detection and differentiation of iron-responsive avirulent mutants on Congo red agar. Infection and Immunity 18:94–98
     [Google Scholar]
  19. Peppier M. S. 1982; Isolation and characterization of isogenic pairs of domed hemolytic and flat nonhemolytic colony types of Bordetella pertussis. Infection and Immunity 35:840–851
     [Google Scholar]
  20. Perkins F. T., Sheffield F. W., Outschoom A. G., Hemsley D. A. 1973; An international collaborative study on the measurement of the opacity of bacterial suspensions. Journal of Biological Standardization 1:1–10
     [Google Scholar]
  21. Pittman M., Furman B. L., Wardlaw A. C. 1980; Bordetella pertussis respiratory tract infection in the mouse: pathophysiological responses. The Journal of Infectious Diseases 142:56–66
     [Google Scholar]
  22. Prpic J. K., Browne R. M., Davey R. B. 1983; Differentiation between virulent and avirulent Yersinia enterocolitica isolates by using Congo red agar. Journal of Clinical Microbiology 18:486–490
     [Google Scholar]
  23. Prpic J. K., Browne R. M., Davey R. B. 1985; In vitro assessment of virulence in Yersinia enterocolitica and related species. Journal of Clinical Microbiology 22:105–110
     [Google Scholar]
  24. Pusztai Z., Joo I. 1967; Influence of nicotinic acid on the antigenic structure of Bordetella pertussis. Annals Immunologiae Hungaricae 10:63–67
     [Google Scholar]
  25. Redhead K., Hill T., Chart H. 1987; Interaction of lactoferrin and transferrins with the outer membrane of Bordetella pertussis. Journal of General Microbiology 133:891–898
     [Google Scholar]
  26. Rimler R. B., Simmons D. G. 1983; Differentiation among bacteria isolated from turkeys with coryza (rhinotracheitis). Avian Diseases 27:491–500
     [Google Scholar]
  27. Robins-Browne R. M., Prpic J. K., Davey R. B. 1986; Influence of the virulence plasmid and the Congo red reaction on the antimicrobial susceptibility of Yersinia species. Journal of Antimicrobial Chemotherapy 17:553–557
     [Google Scholar]
  28. Robinson A., Duggleby C. J., Gorringe A. R., Livey I. 1986; Antigenic variation in Bordetella pertussis. Birkbeck T. H., Penn C. W. (eds) Antigenic variation in infectious diseases IRL Press; Oxford: pp 147–161
     [Google Scholar]
  29. Robinson A., Gorringe A. R., Irons L. I., Keevil C. W. 1983; Antigenic modulation of Bordetella pertussis in continuous culture. FEMS Microbiology Letters 19:105–109
     [Google Scholar]
  30. Schneider D. R., Parker C. D. 1982; Effect of pyridines on phenotypic properties of Bordetella pertussis. Infection and Immunity 38:548–553
     [Google Scholar]
  31. Surgalla M. J., Beesley E. D. 1969; Congo red-agar plating medium for detecting pigmentation in Pasteurella pestis. Applied Microbiology 18:834–837
     [Google Scholar]
  32. Wardlaw A. C., Parton R. 1988; The host-parasite relationship in pertussis. In Wardlaw A. C., Parton R. (eds) Pathogenesis and immunity in pertussis John Wiley and Sons Ltd; Chichester: in press
    [Google Scholar]
  33. Wardlaw A. C., Parton R., Hooker M. J. 1976; Loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of Bordetella pertussis. Journal of Medical Microbiology 9:89–100
     [Google Scholar]
  34. Weiss A. A., Falkow S. 1984; Genetic analysis of phase change in Bordetella pertussis. Infection and Immunity 43:263–269
     [Google Scholar]
  35. Weiss A. A., Hewlett E. L., Myers G. A., Falkow S. 1983; Tn5-induced mutations affecting virulence factors of Bordetella pertussis. Infection and Immunity 42:33–41
     [Google Scholar]
  36. Weiss A. A., Hewlett E. L., Myers G. A., Falkow S. 1984; Pertussis toxin and extracytoplasmic adenylate cyclase as virulence factors of Bordetella pertussis. Journal of Infectious Diseases 150:219–222
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-26-4-301
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Differentiation of phase I and variant strains of Bordetella pertussis on Congo red media
J. Med. Microbiol. 26, 301 (1988); https://doi.org/10.1099/00222615-26-4-301
/content/journal/jmm/10.1099/00222615-26-4-301
/content/journal/jmm/10.1099/00222615-26-4-301
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