The value of adding β-lactamase to bottles of blood-culture medium before their distribution to wards was investigated. Significantly more bottles containing β-lactamase were culture-positive than those without (p< 0.002). In another series, when the enzyme was added to both bottles in each set there was no significant difference in isolation rates between the two bottles. The groups of organisms which were isolated more readily when β-lactamase was present were staphylococci and streptococci. Storage of β-lactamase (Genzyme broad-spectrum mixture) in blood-culture medium at room temperature resulted in rapid loss of cephalosporinase activity, whereas little decline in penicillinase activity was noted over a period of 118 days.
AppelbaumP. C.,
BeckwithD. G.,
DipersioJ. R.,
DykeJ. W.,
SalventiJ. F.,
StoneL. L.1983; Enhanced detection of bacteremia with a new Bactec resin blood culture medium. Journal of Clinical Microbiology 17:48–51
BrownD. F. J.,
WarnerM.,
WarrenR. E.1984; Automated detection of micro-organisms in blood cultures by means of the Malthus Microbiological Growth Analyzer. Journal of Clinical Pathology 37:65–69
CarletonJ.,
FlamburgerM.1963; Unmasking of false-negative blood cultures in patients receiving new penicillins. Journal of the American Medical Association 186:157–159
DoemG. V.,
GantzN. M.1983; Detection of bacteremia in patients receiving antimicrobial therapy: an evaluation of the antimicrobial removal device and 16B medium. Journal of Clinical Microbiology 18:43–48
DowlingH. F.,
HirshH. L.1945; The uses of penicillinase in cultures of body fluids obtained from patients under treatment with penicillin. American Journal of Medical Sciences 210:756–762
EdbergS. C.,
BottenbleyC. J.,
GramK.1976; Use of sodium polyanethol sulfonate to selectively inhibit aminoglycoside and polymyxin antibiotics in a rapid blood level antibiotic assay. Antimicrobial Agents and Chemotherapy 9:414–417
LowranceB. L.,
TraubW. H.1969; Inactivation of the bacterial activity of human serum by Liquoid (sodium polyanethol-sulfonate). Applied Microbiology 17:839–842
LiddellF. D.1983; Simplified exact analysis of case-referent studies: matched pairs: dichotomous exposure. Journal of Epidemiology and Community Health 37:82–84
McGuireN. M.,
KauffmanC. A.,
HertzC. S.,
KovachJ. M.1983; Evaluation of the BACTEL antimicrobial removal system for detection of bacteremia. Journal of Clinical Microbiology 18:449–451
MorelloJ. A.1983; Blood culture practices currently in use. In
BalowsA.,
SonnenwirthA. C.
(eds) Bacteremia, laboratory and clinical aspects. Charles C. Thomas; Springfield: pp 61–95
WallisC.,
MelnickJ. L.,
WendeR. D.,
RielyP. E.1980; Rapid isolation of bacteria from septicemic patients by use of an antimicrobial agent removal device. Journal of Clinical Microbiology 11:462–464
WashingtonJ. A.1978; Conventional approaches to blood culture. In
WashingtonJ. A.
(ed) The detection of septicemia CRC Press, West Palm Beach; Florida: pp 41–87