Monoclonal antibodies to the lipopolysaccharide (LPS) core region were produced by immunising mice with strain J5 (chemotype Rc). One of these bound to the deepest part of the core, i.e., Lipid A, and reacted with other heat-killed but not live gram-negative bacilli, including and . Eight other monoclonal antibodies, binding to the terminal glucose residue of Rc LPS, reacted with live cells of strains only. Thus, the O antigen does not necessarily render the core inaccessible to antibody. However, despite binding to live bacteria, these monoclonal antibodies neither enhanced phagocytic killing, nor protected mice from dying from gram-negative infection or endotoxaemia. It is concluded that antibodies reacting with the most immunodominant parts of the J5 core are not protective.


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