Serum samples from patients infected with serovar were tested by the microscopic agglutination test (MAT), enzyme immunoassay (EIA) and immunoblotting. There was no apparent correlation between MAT titre and EIA optical density (OD) for individual serum samples, but sequential serum samples produced similar profiles in both tests during the course of an infection. Immunoblotting of sonicate with patients' sera revealed reactions with a number of bands, in the mol. wt (103) range 14-4-95. However, all serum samples reacted with the major 28 x 10-mol. wt sub-unit of lipopolysaccharide (LPS) and most reacted with a (34·5-35) x 10-mol. wt flagella doublet. Examination of sequential serum samples obtained over a period of about 3 months after infection revealed little change in the antigens detected after the second to third week of infection. Absorption of patients' sera with whole viable leptospires revealed that antibodies to several exposed antigens, including LPS, were produced. Sera which reacted with flagella also reacted with bands of similar mol. wts in preparations from other serovars.


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