1887

Abstract

Summary.

Faecal specimens from 66 children with haemolytic uraemic syndrome in the United Kingdom were examined for strains of producing Vero cytotoxin (VT). Initially, conventional bacteriological methods were used to identify colonies of which were then tested for VT production. Subsequently, specific DNA probes for VT1 and VT2 were used in hybridisation tests to detect VT-producing E. coli (VTEC). VTEC strains were isolated from 19 cases and in 15 they belonged to serogroup O157. Fourteen of these O157 strains possessed the flagellar antigen H7 and one was non-motile. The VTEC strains from the remaining four cases belonged to serotypes O26:H11, O104:H2, O153:H25, and O163:H19 together with a rough VT strain with flagellar antigen H51. The O157 strains hybridised with either the VT2 probe or both VT1 and VT2 probes. The other VTEC strains hybridised with either the VT1 or VT2 probe. Confirmation of the production of VT1 and VT2 was obtained by the neutralisation of faecal VT with specific antisera raised against these two cytotoxins.

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1988-04-01
2024-04-24
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References

  1. Anderson E S, Threlfall E J. 1974; The characterisation of plasmids in the enterobacteria. Journal of Hygiene 72:471–487
    [Google Scholar]
  2. Cleary T G, Mathewson J J, Faris E, Pickering L K. 1985; Shiga-like cytotoxin production by enteropathogenic Escherichia coli serogroups. Infection and Immunity 47:335–337
    [Google Scholar]
  3. Cowan S T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. , 2. Cambridge University Press;
    [Google Scholar]
  4. Day N P, Scotland S M, Rowe B. 1981; Comparison of an HEp-2 tissue culture test with the Serény test for detection of enteroinvasiveness in Shigella spp. and Escherichia coli . Journal of Clinical Microbiology 13:596–597
    [Google Scholar]
  5. Drummond K N. 1985; Hemolytic uremic syndrome-then and now. New England Journal of Medicine 312:116–118
    [Google Scholar]
  6. Farmer J J, Davis B R. 1985; H7 Antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology 22:620–625
    [Google Scholar]
  7. Gross R J, Rowe B. 1985; Serotyping of Escherichia coli . Sussman M. The virulence of Escherichia coli Reviews and Methods Academic Press; London:345–363
    [Google Scholar]
  8. Gross R J, Scotland S M, Rowe B. 1976; Enterotoxin testing of Escherichia coli causing epidemic infantile enteritis in the UK. Lancet 1:629–631
    [Google Scholar]
  9. Gully P R. 1984; Haemolytic-uraemic syndrome : epidemiology and report of an outbreak. Journal of the Royal Society of Health 104:214–217
    [Google Scholar]
  10. Huang A. et al 1986; Cloning and expression of the genes specifying Shiga-like toxin production in Escherichia coli H 19. Journal of Bacteriology 166:375379
    [Google Scholar]
  11. Johnson W M, Lior H, Bezanson G S. 1983; Cytotoxic Escherichia coli O157 : H7 associated with haemorrhagic colitis in Canada. Lancet1–76
    [Google Scholar]
  12. Karmali M A, Petric M, Lim C, Fleming P C, Arbus G S, Lior H. 1985; The association between idiopathic hemolytic uremic syndrome and infection by Verotoxin-producing Escherichia coli. Journal of Infectious Diseases 151:775–782
    [Google Scholar]
  13. Karmali M A, Petric M, Louie S, Cheung R. 1986; Antigenic heterogeneity of Escherichia coli Verotoxins. Lancet 1:164–165
    [Google Scholar]
  14. Karmali M A, Steele B T, Petric M, Lim C. 1983; Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 1:619–620
    [Google Scholar]
  15. Konowalchuk J, Dickie N, Stavric S, Speirs J I. 1978a; Properties of an Escherichia coli cytotoxin. Infection and Immunity 20:575–577
    [Google Scholar]
  16. Konowalchuk J, Dickie N, Stavric S, Speirs J I. 1978b; Comparative studies of five heat-labile toxic products of Escherichia coli. Infection and Immunity 22:644–648
    [Google Scholar]
  17. Konowalchuk J, Speirs J I, Stavric S. 1977; Vero response to a cytotoxin of Escherichia coli. Infection and Immunity 18:775–779
    [Google Scholar]
  18. Koster F. et al 1978; Hemolyticuremic syndrome after shigellosis : Relation to endotoxemia and circulating immune complexes. New England Journal of Medicine 298:927–933
    [Google Scholar]
  19. Levin M, Barrait J M. 1984; Haemolytic uraemic syndrome. Archives of Disease in Childhood 59:397–400
    [Google Scholar]
  20. Newland J W, Strockbine N A, Miller S F, O’Brien A D, Holmes R K. 1985; Cloning of Shiga-like toxin structural genes from a toxin converting phage of Escherichia coli. Science 230:179–181
    [Google Scholar]
  21. O’Brien A D, LaVeck G D, Thompson M R, Formal S B. 1982; Production of Shigella dysenteriae type 1-like cytotoxin by Escherichia coli. Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  22. O’Brien A D, Newland J W, Miller S F, Holmes R K, Smith H W, Formal S B. 1984; Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226:694–696
    [Google Scholar]
  23. Rahaman M M, Jamial Alam A K M, Islam M R, Greenough W B, Lindenbaum J. 1975; Shiga bacillus dysentery associated with marked leukocytosis and erythrocyte fragmentation. Johns Hopkins Medical Journal 136:65–70
    [Google Scholar]
  24. Riley L W. et al 1983; Hemorrhagic colitis associated with a rare Escherichia coli serotype. New England Journal of Medicine 308:681–685
    [Google Scholar]
  25. Scotland S M, Day N P, Rowe B. 1980; Production of a cytotoxin affecting Vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups. FEMS Microbiology Letters 7:15–17
    [Google Scholar]
  26. Scotland S M, Smith H R, Rowe B. 1985; Two distinct toxins active on Vero cells from Escherichia coli O157. Lancet 2:885–886
    [Google Scholar]
  27. Scotland S M, Smith H R, Willshaw G A, Rowe B. 1983; Vero cytotoxin production in strain of Escherichia coli is determined by genes carried on bacteriophage. Lancet 2:216
    [Google Scholar]
  28. Smith H R, Day N P, Scotland S M, Gross R J, Rowe B. 1984; Phage-determined production of Vero cytotoin in strains of Escherichia coli serogroup O157. Lancet 1:1242–1243
    [Google Scholar]
  29. Smith H W, Green P, Parsell Z. 1983; Vero cell toxins in Escherichia coli and related bacteria : transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs. Journal of General Microbiology 129:3121–3137
    [Google Scholar]
  30. Taylor C M, White R H R, Winterborn M H, Rowe B. 1986; Haemolytic-uraemic syndrome : clinical experience of an outbreak in the West Midlands. British Medical Journal 292:1513–1516
    [Google Scholar]
  31. Von Gasser C, Gautier E, Steck A, Siebenmann R E, Oechslin R. 1955; Hämolytisch-urämische syndrome : bilaterale nierenrindennekrosen bei akuten erworbenen hämolytischen anämien. Schweizerische Medizinische Wochenschrift 85:905–909
    [Google Scholar]
  32. Wells J G. et al 1983; Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. Journal of Clinical Microbiology 18:512–520
    [Google Scholar]
  33. Willshaw G A, Smith H R, Scotland S M, Field A M, Rowe B. 1987; Heterogeneity of Escherichia coli phages encoding Vero cytotoxins : Comparison of cloned sequences determining VT1 and VT2 and development of specific gene probes. Journal of General Microbiology 133:1309–1317
    [Google Scholar]
  34. Willshaw G A, Smith H R, Scotland S M, Rowe B. 1985; Cloning of genes determining the production of Vero cytotoxin by Escherichia coli. Journal of General Microbiology 131:3047–3053
    [Google Scholar]
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