1887

Abstract

Summary.

Mice surviving a sublethal O18:K1 infection possess a greatly increased resistance to a subsequent lethal O18:K1 peritonitis. A similar increase in resistance can also be achieved by LPS pretreatment. The early course of the infection in the convalescent mice at day 1 was identical to that in LPS-pretreated mice. At this time, the convalescent mice were also able to restrict the growth of the heterologous O78(ColV) strain, suggesting that non-specific phagocyte activation was responsible for the increased destruction of the bacteria. At day 4, the kinetics of infection in convalescent mice were identical to those in mice passively immunised with specific anti-K1 capsule antiserum. A rapid decline in the numbers of viable homologous, but not heterologous, bacteria took place in the peritoneal cavity suggesting effective antibody-mediated opsonisation followed by phagocytosis and killing of the bacteria.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-3-205
1988-03-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/3/medmicro-25-3-205.html?itemId=/content/journal/jmm/10.1099/00222615-25-3-205&mimeType=html&fmt=ahah

References

  1. Abernathy R S. 1957; Homologous and heterologous resistance in mice given bacterial endotoxins. Journal of Immunology 78:387–394
    [Google Scholar]
  2. Dunn D L, Barke R A, Knight N B, Humphrey E W, Simmons R L. 1985; Role of resident macrophages, peripheral neutrophils and translymphatic absorption in bacterial clearance from the peritoneal cavity. Infection and Immunity 49:257–264
    [Google Scholar]
  3. Galanos C, Liideritz O, Westphal O. 1969; A new method for the extraction of R lipopolysaccharides. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  4. Galelli A, Parant M, Chedid L. 1977; Role of radiosensitive and radioresistant cells in nonspecific resistance to infection of LPS-treated mice. Journal of the Reticuloendothelial Society 21:109–118
    [Google Scholar]
  5. Horwitz M A, Silverstein S C. 1980; Influence of the Escherichia coli capsule on complement fixation and on phagocytosis and killing by human phagocytes. Journal of Clinical Investigation 65:82–94
    [Google Scholar]
  6. Landy M. 1956; Increase in resistance following administration of bacterial lipopolysaccharides. Annals of the New York Academy of Sciences 66:292–303
    [Google Scholar]
  7. Miller J H. 1972; Experiments in molecular genetics. Cold Spring Harbor Laboratory; New York:
    [Google Scholar]
  8. Parant M. 1983; Effect of LPS on nonspecific resistance to bacterial infections. Nowotny A. Beneficial effects of endotoxins Plenum Press; New York:179–196
    [Google Scholar]
  9. Reed L J, Muench H. 1938; A simple method of estimating fifty per cent end-points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  10. Rowley D. 1955; Stimulation of natural immunity to Escherichia coli infections. Observations on mice. Lancet 1:232–234
    [Google Scholar]
  11. Rowley D. 1964; Endotoxin-induced changes in susceptibility to infections. Landy M, Braun W. Bacterial endotoxins Rutgers University Press; New Brunswick, NJ:359–372
    [Google Scholar]
  12. Tegtmeier B R, Andersen B R. 1983; Mechanisms of lipopolysac-charide-induced protection against Pseudomonas sepsis in granulocytopenic mice. Reviews of Infectious Diseases 5: Suppl S963–970
    [Google Scholar]
  13. Vaara M, Viljanen P, Vaara T, Mâkelâ P H. 1984; An outer membrane-disorganizing peptide PMBN sensitizes E. coli strains to serum bactericidal action. Journal of Immunology 132:2582–2589
    [Google Scholar]
  14. Vuopio-Varkila J, Karvonen M, Saxén H. 1987a; The protective capacity of antibodies to various outer membrane components in a systemic mouse peritonitis model caused by Escherichia coli O18: K1. Journal of Medical Microbiology. In press
    [Google Scholar]
  15. Vuopio-Varkila J, Nurminen M, Pyhala L, Makela P H. 1987b; LPS-induced nonspecific resistance to systemic E. coli infection in mice. Journal of Medical Microbiology 25:197–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-3-205
Loading
/content/journal/jmm/10.1099/00222615-25-3-205
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error