1887

Journal of Medical Microbiology logo

Volume 25, Issue 3

Lipopolysaccharide-induced non-specific resistance to systemic infection in mice Free

Abstract

Summary.

A high degree of non-specific resistance to a lethal systemic infection was induced in mice by pretreatment with a small dose (< 5 g/mouse) of the homologous lipopolysaccharide (LPS) or with heterologous rough-type LPS from K-12. The route of LPS administration, intraperitoneally or subcutaneously, did not influence the development of resistance, suggesting that a systemic cell activation was responsible for the effect. The enhanced elimination of bacteria was similar to that in mice recovering from a sublethal infection. In the LPS-treated mice, elimination of the challenge bacteria from the peritoneal cavity and the blood started 3–4 h after challenge whereas, in controls, the bacterial numbers continued to increase until the mice died. The detoxified LPS derivative, monophosphoryl lipid A (MPL), also increased the survival of mice infected with O18 :K1. However, the dose of MPL required for optimal infection resistance was 100-fold greater than that of native, K-12 LPS, corresponding to the 100-fold reduced toxicity of MPL for mice and rabbits in lethality and pyrogenicity assays.

  • Received:
  • Accepted:
  • Published Online:
© 1988 Pathological Society of Great Britain and Ireland
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-3-197
1988-03-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/3/medmicro-25-3-197.html?itemId=/content/journal/jmm/10.1099/00222615-25-3-197&mimeType=html&fmt=ahah

References

  1. Abernathy R S. 1957; Homologous and heterologous resistance in mice given bacterial endotoxins. Journal of Immunology 78:387–394
     [Google Scholar]
  2. Antoni G. 1986; A short synthetic peptide fragment of human interleukin 1 with immunostimulatory but not inflammatory activity. Journal of Immunology 137:3201–3204
     [Google Scholar]
  3. Chase J J. 1986; Effect of monophosphoryl lipid A on host resistance to bacterial infection. Infection and Immunity 53:711–712
     [Google Scholar]
  4. Chedid L, Parant M, Damais C, Parant F, Juy D, Galelli A. 1976; Failure of endotoxin to increase nonspecific resistance to infection of lipopolysaccharide low-responder mice. Infection and Immunity 13:722–727
     [Google Scholar]
  5. Cluff L E. 1970; Effects of endotoxins on susceptibility to infections. Journal of Infectious Diseases 122:205–215
     [Google Scholar]
  6. Duff G W, Durum S K. 1983; The pyrogenic and mitogenic actions of interleukin-1 are related. Nature 304:449–451
     [Google Scholar]
  7. European pharmacopoeia 1971; II Maisonneuve; France:53–61
  8. Galanos C, Luderitz O, Westphal O. 1969; A new method for the extraction of R lipopolysaccharides. European Journal of Biochemistry 9:245–249
     [Google Scholar]
  9. Galanos C, Freudenberg M A, Reutter W. 1979; Galactosamine-induced sensitization to the lethal effects of endotoxin. Proceedings of the National Academy of Sciences of the USA 76:5939–5943
     [Google Scholar]
  10. Galelli A, Parant M, Chedid L. 1977; Role of radiosensitive and radioresistant cells in nonspecific resistance to infection of LPS-treated mice. Journal of the Reticuloendothelial Society 21:109–118
     [Google Scholar]
  11. Gery I, Waksman B H. 1972; Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). Journal of Experimental Medicine 136:143–155
     [Google Scholar]
  12. Landy M. 1956; Increase in resistance following administration of bacterial lipopolysaccharides. Annals of the New York Academy of Sciences 66:292–303
     [Google Scholar]
  13. Luderitz O, Galanos C, Lehmann V, Mayer H, Rietschel E T, Weckesser J. 1978; Chemical structure and biological activities of lipid A’s from various bacterial families. Naturwissenschaften 65:578–585
     [Google Scholar]
  14. Madonna G S, Peterson J E, Ribi E, Vogel S N. 1986; Early-phase endotoxin tolerance: induction by a detoxified lipid A derivative, monophosphoryl lipid A. Infection and Immunity 52:6–11
     [Google Scholar]
  15. Masihi K N, Lange W, Johnson A G, Ribi E. 1986; Enhancement of chemiluminescence and phagocytic activities by nontoxic and toxic forms of lipid A. Journal of Biological Response Modifiers 5:A62–469
     [Google Scholar]
  16. Miller J H. 1972 Experiments in molecular genetics Cold Spring Harbor Laboratory; New York:
     [Google Scholar]
  17. Munford R S, Hall C L. 1986; Detoxification of bacterial lipopolysaccharides (endotoxins) by a human neutrophil enzyme. Science 234:203–205
     [Google Scholar]
  18. Nowotny A. 1985; Antitumor effects of endotoxin. Berry L J. Handbook of endotoxins 3 Cellular biology of endotoxin, Elsevier; Amsterdam:389–448
     [Google Scholar]
  19. Parant M. 1983; Effect of LPS on nonspecific resistance to bacterial infections. Nowotny A. Beneficial Effects of Endotoxins, Plenum Press; New York:179–196
     [Google Scholar]
  20. Reed L J, Muench H. 1938; A simple method of estimating fifty per cent end-points. American Journal of Hygiene 27:493–497
     [Google Scholar]
  21. Ribi E. 1984; Beneficial modification of the endotoxin molecule. Journal of Biological Response Modifiers 3:1–9
     [Google Scholar]
  22. Ribi E, Cantrell J, Feldner T, Myers K, Peterson J. 1986; Biological activities of monophosphoryl lipid A. Leive L. Microbiology 1986 American Society for Microbiology; Washington D.C.:9–13
     [Google Scholar]
  23. Rosenstreich D L. 1985; Genetic control of endotoxin response: C3H/HeJ mice. Berry L J. Handbook of endotoxins 3 Cellular biology of endotoxin. Elsevier; Amsterdam:82–122
     [Google Scholar]
  24. Rowley D. 1955; Stimulation of natural immunity to Escherichia coli infections. Observations on mice. Lancet 1:232–234
     [Google Scholar]
  25. Tegtmeier B R, Andersen B R. 1983; Mechanisms of lipopolysac-charide-induced protection against Pseudomonas sepsis in granulocytopenic mice. Reviews of Infectious Diseases 5: Suppl S963–S969
     [Google Scholar]
  26. Vaara M, Viljanen P, Vaara T, Makela P H. 1984; An outer membrane-disorganizing peptide PMBN sensitizes E. coli strains to serum bactericidal action. Journal of Immunology 132:2582–2589
     [Google Scholar]
  27. Vuopio-Varkila J. 1988; Experimental Escherichia coli peritonitis in immunosuppressed mice; the role of specific and nonspecific resistance mechanisms. Journal of Medical Microbiology 25:33–39
     [Google Scholar]
  28. Vuopio-Varkila J, Karvonen M, Saxén H. 1988; The protective capacity of antibodies to various outer membrane components in a systemic mouse peritonitis model caused by Escherichia coli O18 :K1. Journal of Medical Microbiology In press
    [Google Scholar]
  29. Westphal O, Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-3-197
Loading
Lipopolysaccharide-induced non-specific resistance to systemic Escherichia coli infection in mice
J. Med. Microbiol. 25, 197 (1988); https://doi.org/10.1099/00222615-25-3-197
/content/journal/jmm/10.1099/00222615-25-3-197
/content/journal/jmm/10.1099/00222615-25-3-197
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error