1887

Abstract

Summary.

Two hybridoma cell lines that produce monoclonal antibodies against haemolysin were established by fusion of myeloma and spleen cells obtained from a mouse immunised with haemolysin detoxified with tetranitro-methane. Enzyme-linked immunosorbent assay (ELISA) showed that the two purified monoclonal antibodies, B7 and Bl 1, recognised the same epitope on the haemolysin molecule. Antibody B7 neutralised the haemolytic and enterotoxic activities of the haemolysin. It is concluded that the same site on the haemolysin molecule is responsible for both haemolytic and enterotoxic activities.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-3-187
1988-03-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/3/medmicro-25-3-187.html?itemId=/content/journal/jmm/10.1099/00222615-25-3-187&mimeType=html&fmt=ahah

References

  1. Asao T, Kinoshita Y, Kozaki S, Uemura T, Sakaguchi G. 1984; Purification and some properties of Aeromonas hydrophila hemolysin . Infection and Immunity 46:122–127
    [Google Scholar]
  2. Asao T. 1986; Purification and characterization of an Aeromonas hydrophila hemolysin . Journal of Clinical Microbiology 24:228–232
    [Google Scholar]
  3. Barer M R, Millership S E, Tabaqchali S. 1986; Relationship of toxin production to species in the genus Aeromonas . Journal of Medical Microbiology 22:303–309
    [Google Scholar]
  4. Bunning V K, Crawford R G, Stelma G N, Kaylor L O, Johnson C H. 1986; Melanogenesis in murine B16 cells exposed to Aeromonas hydrophila cytotoxic enterotoxin. Canadian Journal of Microbiology 32:814–819
    [Google Scholar]
  5. Burke V, Gracey M, Robinson J, Peck D, Beaman J, Bundell C. 1983; The microbiology of childhood gastroenteritis: Aeromonas species and other infective agents. Journal of Infectious Diseases 148:68–74
    [Google Scholar]
  6. Cuatrecasas P, Fuchs S, Anfinsen C B. 1968; The tyrosyl residues at the active site of staphylococcal nuclease. Modifications by tetranitromethane. Journal of Biological Chemistry 243:4787–4798
    [Google Scholar]
  7. Cumberbatch N, Gurwith M J, Langston C, Sack R B, Brunton J L. 1979; Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infection and Immunity 23:829–837
    [Google Scholar]
  8. Dean A G, Ching Y C, Williams R G, Harden L B. 1972; Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. Journal of Infectious Diseases 125:407–411
    [Google Scholar]
  9. Donta S T, Haddow A D. 1978; Cytotoxic activity of Aeromonas hydrophila . Infection and Immunity 21:989–993
    [Google Scholar]
  10. George W L, Nakata M M, Thompson J, White M L. 1985; Aeromonas-related diarrhea in adults. Archives of Internal Medicine 145:2207–2211
    [Google Scholar]
  11. Gracey M, Burke V, Robinson J. 1982; Aeromonas-associated gastroenteritis. Lancet 2:1304–1306
    [Google Scholar]
  12. Holmberg S D, Farmer J J. 1984; Aeromonas hydrophila and Plesiomonas shigelloides as causes of intestinal infections. Reviews of Infectious Diseases 6:633–639
    [Google Scholar]
  13. Janda J M, Reitano M, Bottone E J. 1984; Biotyping of Aeromonas isolates as a correlate to delineating a species-associated disease spectrum. Journal of Clinical Microbiology 19:44–47
    [Google Scholar]
  14. Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Millership S E, Barer M R, Tabaqchali S. 1986; Toxin production by Aeromonas spp. from different sources. Journal of Medical Microbiology 22:311–314
    [Google Scholar]
  16. Nakane P K, Kawaoi A. 1974; Peroxidase-labeled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  17. Pitarangsi C. 1982; Enteropathogenicity of Aeromonas hydrophila and Plesiomonas shigelloides: prevalence among individuals with and without diarrhea in Thailand. Infection and Immunity 35:666–673
    [Google Scholar]
  18. Stelma G N, Johnson C H, Spaulding P. 1986; Evidence for the direct involvement of β-hemolysin in Aeromonas hydrophila enteropathogenicity. Current Microbiology 14:71–77
    [Google Scholar]
  19. Turnbull P C B. 1984; Enterotoxin production in relation totaxonomic grouping and source of isolation of Aeromonas species. Journal of Clinical Microbiology 19:175–180
    [Google Scholar]
  20. Wretlind B, Héden L, Wadstrom T. 1973; Formation of extracellular haemolysin by Aeromonas hydrophila in relation to protease and staphylolytic enzyme. Journal of General Microbiology 78:57–65
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-3-187
Loading
/content/journal/jmm/10.1099/00222615-25-3-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error