1887

Abstract

Summary

The susceptibility of 76 clinical isolates of Enterobacteriaceae to ampicillin, piperacillin and gentamicin was assessed by ATP bioluminescence in a 4-h test. For most organisms tested ( and ), there was good correlation with traditional MIC values estimated on 18-h cultures. However strains of showed false resistance to the -lactam agents with the ATP method; and concordance was achieved only after manipulation of the growth conditions. Our method is simpler than those described previously, though currently it is still labour-intensive and expensive.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-2-95
1988-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/2/medmicro-25-2-95.html?itemId=/content/journal/jmm/10.1099/00222615-25-2-95&mimeType=html&fmt=ahah

References

  1. Bascomb S, Glynn A A, Gaya H, Spencer R C, Shine P J. 1982; Rapid determination of bacterial susceptibility to antimicrobial agents by a semi-automated continuous flow method. Journal of Antimicrobial Chemotherapy 9:343–55
    [Google Scholar]
  2. Beckers B, Lang H R M, Beinke A. 1984; Determination of intracellular ATP during growth of Escherichia coli in the presence of ampicillin. Kricka L J. Analytical applications of bioluminescence and chemiluminescence Academic Press; London:67–70
    [Google Scholar]
  3. Colvin H J, Sherris J C. 1977; Electrical impedance measurements in the reading and monitoring of broth dilution susceptibility tests. Antimicrobial Agents and Chemotherapy 12:61–6
    [Google Scholar]
  4. Deblanc H J, Charache P, Wagner H N. 1972; Automated radiometrie measurement of antibiotic effect on bacterial growth. Antimicrobial Agents and Chemotherapy 2:360–6
    [Google Scholar]
  5. D’Eustachio A J, Johnson D R. 1968; Adenosine triphosphate content of bacteria. Federation Proceedings 27:761
    [Google Scholar]
  6. DeLuca M. 1986; Firefly luciferase. Journal of Bioluminescence and Chemiluminescence 1:94
    [Google Scholar]
  7. Greenwood D, O’Grady F. 1972; The effect of osmolality on the response of Escherichia coli and Proteus mirabilis to penicillins. British Journal of Experimental Pathology 53:457–64
    [Google Scholar]
  8. Greenwood D, O’Grady F. 1973; Comparison of the responses of Escherichia coli and Proteus mirabilis to seven β-lactam antibiotics. Journal of Infectious Diseases 128:211–22
    [Google Scholar]
  9. Gutekunst R R. 1977; Luminescence assays for the determination of microbial levels. Proceedings of the ATP methodology symposium SAI Technology Co; San Diego:491–524
    [Google Scholar]
  10. Hastings J G M. Luminescence in clinical microbiology. Schölmerich J. Bioluminescence and chemiluminescenceProceedings of the 4th International Symposium John Wiley; Chichester: in press
    [Google Scholar]
  11. Hobby G L, Dawson M H. 1944; Effect of rate of growth of bacteria on action of penicillin. Proceedings of the Society for Experimental Biology and Medicine 56:181–184
    [Google Scholar]
  12. Hojer H, Nilsson L, Ansehn S, Thore A. 1976; In-vitro effect of doxycycline on levels of adenosine triphosphate in bacterial cultures : possible clinical applications. Scandinavian Journal of Infectious Diseases Suppl 9:58–61
    [Google Scholar]
  13. Kouda M, Ouchi Y, Takasaki Y, Maeda T, Matsuzaki H, Nakaya R. 1985; Bioluminescent assay as a potential method of rapid susceptibility testing. Microbiology and Immunology 29:309–315
    [Google Scholar]
  14. Martin E T, Cote J A, Perry L K, Martin W J. 1985; Clinical evaluation of the Lumac bioluminescence method for screening urine specimens. Journal of Clinical Microbiology 22:19–22
    [Google Scholar]
  15. Martin H H. 1984; In-vitro synthesis of peptidoglycan by spherophasts of Proteus mirabilis grown in the presence of penicillin. Archives of Microbiology 139:371–75
    [Google Scholar]
  16. McWalter P W. 1984; Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin-luciferase assay of bacterial adenosine triphosphate. Journal of Applied Bacteriology 56:145–50
    [Google Scholar]
  17. Nilsson L. 1982; Rapid bioluminescent assay of serum amikacin. Journal of Antimicrobial Chemotherapy 10:125–30
    [Google Scholar]
  18. Picciolo G L, Chappelle E W. 1977; Application of firefly luciferase assay for adenosine triphosphate (ATP) to antimicrobial drug sensitivity testing. NASA Technical Note D-8439
    [Google Scholar]
  19. Porter I A, Reid T M S, Wood W J, Gibson D M, Hobbs G. 1983; Conductance measurements for determining antibiotic sensitivity. Russell A D, Quesnel L B. Antibiotics: assessment of antimicrobial activity and resistance Academic Press; London:49–60
    [Google Scholar]
  20. Spencer R C, Wheat P F. 1986; Novel mechanisms for determining antibiotic susceptibilities. Journal of Antimicrobial Chemotherapy 17:404–407
    [Google Scholar]
  21. Thore A, Nilsson L, Hojer H, Ansehn S, Brote L. 1977; Effects of ampicillin on intracellular levels of adenosine triphosphate in bacterial cultures related to antibiotic susceptibility. Acta Pathologica Microbiologica et Immunologica Scandinavica 85B:161–6
    [Google Scholar]
  22. Tuomanen E, Cozens R, Tosch W, Zak O, Tomasz A. 1986; The rate of killing of Escherichia coli by β-lactam antibiotics is strictly proportional to the rate of bacterial growth. Journal of General Microbiology 132:1297–1304
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-2-95
Loading
/content/journal/jmm/10.1099/00222615-25-2-95
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error