1887

Abstract

Summary

Murine monoclonal antibodies and immune rabbit serum were raised against the rough mutant strain R595. These antibodies were tested for their opsonic activity against the homologous strain and the smooth wild type by luminol-dependent chemiluminescence and a microscopic assessment of phagocytosis. Immune rabbit serum opsonised both strains. Treatment with normal rabbit serum inhibited the phagocytic uptake of R595. None of the monoclonal antibodies RE01 (anti-KDO), RE12 (anti-KDO) and RE23 (anti-lipid A) were opsonic. Unopsonised R595 stimulated marked chemiluminescence possibly because of its hydrophobic surface, but this was not reflected in increased uptake by phagocytic cells. Results obtained with luminoldependent chemiluminescence should be interpreted with caution when the opsonisation of rough bacterial strains or those with high surface hydrophobicity is being investigated.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-2-85
1988-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/2/medmicro-25-2-85.html?itemId=/content/journal/jmm/10.1099/00222615-25-2-85&mimeType=html&fmt=ahah

References

  1. Baumgartner J D. 1985; Prevention of gram-negative shock and death in surgical patients by antibody to endotoxin core glycolipid. Lancet 2:59–63
    [Google Scholar]
  2. Braude A I, Ziegler E J, Douglas H, McCutchan J A. 1977; Antibody to cell wall glycolipid of gram-negative bacteria: Induction of immunity to bacteremia and endotoxemia. Journal of Infectious Diseases 136:S167–S173
    [Google Scholar]
  3. Clark L A, Easmon C S F. 1986; Opsonic activity of intravenous immunoglobulin preparations against Staphylococcus epidermidis . Journal of Clinical Pathology 39:856–860
    [Google Scholar]
  4. Crowley J P, Zinner S H, Peter G. 1982; Opsonization of serum-sensitive and serum-resistant Escherichia coli by rough mutant (Re) antisera. Journal of Laboratory and Clinical Medicine 99:197–205
    [Google Scholar]
  5. Dunn D L, Bogard W C, Cerra F B. 1985; Enhanced survival during murine gram-negative bacterial sepsis by use of a murine monoclonal antibody. Archives of Surgery 120:50–53
    [Google Scholar]
  6. Easmon C S F, Cole P J, Williams A J, Hastings M. 1980; The measurement of opsonic and phagocytic function by luminol-dependent chemiluminescence. Immunology 41:67–74
    [Google Scholar]
  7. Galanos C, Luderitz O, Westphal O. 1969; A new method for the extraction of R-lipopolysaccharides. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  8. Galanos C, Luderitz O, Westphal O. 1971; Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. European Journal of Biochemistry 24:116–122
    [Google Scholar]
  9. Galanos C, Rietschel E T, Luderitz O, Westphal O, Kim Y B, Watson D W. 1972; Biological activities of lipid A complexed with bovine-serum albumin. European Journal of Biochemistry 31:230–233
    [Google Scholar]
  10. Galfre G, Milstein C. 1982; Chemical typing of human kappa light chain subgroups expressed by human hybrid myelomas. Immunology 45:125–128
    [Google Scholar]
  11. Greisman S E, Dubuy J B, Woodward C L. 1978; Experimental gram-negative bacterial sepsis : Re-evaluation of the ability of rough mutant antisera to protect mice. Proceedings of the Society for Experimental Biology and Medicine 158:482–490
    [Google Scholar]
  12. Ivanyi J, Davies P. 1980; Monoclonal antibodies against human growth hormone. Molecular immunology 17:287–290
    [Google Scholar]
  13. Johns M, Skehill A, McCabe W R. 1983; Immunization with rough mutants of Salmonella minnesota. IV. Protection by antisera to O and rough antigens against endotoxin. Journal of Infectious Diseases 147:57–67
    [Google Scholar]
  14. Kirkland T N, Ziegler E J. 1984; An immunoprotective monoclonal antibody to lipopolysaccharide. Journal of Immunology 132:2590–2592
    [Google Scholar]
  15. Kreger B E, Craven D E, Carting P C, McCabe W R. 1980a; Gram-negative bacteremia. III. Reassessment of etiology, epidemiology and ecology in 612 patients. American Journal of Medicine 68:332–343
    [Google Scholar]
  16. Kreger B E, Craven D E, McCabe W R. 1980b; Gram-negative bacteremia. IV. Re-evaluation of clinical features and treatment in 612 patients. American Journal of Medicine 68:344–355
    [Google Scholar]
  17. McCabe W R, Burns S C, Craven D E, Johns M. 1977; Cross-reactive antigens: their potential for immunization-induced immunity to gram-negative bacteria. Journal of Infectious Diseases 136:S161–S166
    [Google Scholar]
  18. McCutchan J A, Wolf J L, Ziegler E J, Braude A I. 1983; Ineffectiveness of single-dose human antiserum to core glycolipid (Escherichia coli J5) for prophylaxis of bacteremic, gram-negative infections in patients with prolonged neutro-penia. Schweizerische Medizinische Wochenschrift1440–45
    [Google Scholar]
  19. Nelles M J, Niswander C A. 1984; Mouse monoclonal antibodies reactive with J5 lipopolysaccharide exhibit extensive serological cross-reactivity with a variety of gram-negative bacteria. Infection and Immunity 46:677–681
    [Google Scholar]
  20. Ng A, Chen C L H, Chang C M, Nowotny A. 1976; Relationship of structure to function in bacterial endotoxins : Serologi-cally cross-reactive components and their effect on protection of mice against some gram-negative infections. Journal of General Microbiology 94:107–116
    [Google Scholar]
  21. Pennington J E, Menkes E. 1981; Type-specific vs cross-protective vaccination for gram-negative bacterial pneumonia. Journal of Infectious Diseases 144:599–603
    [Google Scholar]
  22. Polin R A, Harris C M. 1985; Monoclonal antibodies against Group B streptococcus. Macario A J L, Macario E C. Monoclonal antibodies against bacteria 1 Academic Press; Orlando, FL:37–58
    [Google Scholar]
  23. Rietschel E T, Sidorczyk Z, Zahringer U, Wollenweber H-W, Luderitz O. , 1983; Analysis of the primary structure of lipid A. Anderson L, Unger F M. Bacterial lipopolysaccharides. Structure, synthesis, biological activities American Chemical Society Symposium Series 231, American Chemical Society; Washington, D.C:195–218
    [Google Scholar]
  24. Rosenberg M, Gutnick D, Rosenberg E. 1980; Adherence of bacteria to hydrocarbons : A simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  25. Taylor R L, Conrad H E. 1972; Stoichiometric depolymerization of polyuronides and glucosaminoglycuronans to monosac-charides following reduction of their carbodiimide-acti-vated carboxyl groups. Biochemistry 11:1383–1388
    [Google Scholar]
  26. Teng N N H. 1985; Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies. Proceedings of the National Academy of Sciences of the USA 82:1790–1794
    [Google Scholar]
  27. Van Dijk W C, Verbrugh H A, Van Erne-Van Der Tol M E, Peters R, Verhoef J. 1981; Escherichia coli antibodies in opsonisation and protection against infection. Journal of Medical Microbiology 14:381–389
    [Google Scholar]
  28. Westphal O, Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Whistler R L. Methods in Carbohydrate Chemistry 5 Academic Press; New York:83–91
    [Google Scholar]
  29. Wolff S M. 1982; The treatment of gram-negative bacteremia and shock (editorial). New England Journal of Medicine 307:1267–1268
    [Google Scholar]
  30. Ziegler E J, Douglas H, Sherman J E, Davis C E, Braude A I. 1973; Treatment of E. coli and Klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-Gal epimerase-deficient mutant. Journal of Immunology 111:433–438
    [Google Scholar]
  31. Ziegler E J, McCutchan J A, Fierer J, Glauser M P, Sadof F J C, Douglas H, Braude A I. 1982; Treatment of gram negative bacteraemia and shock with human antiserum to a mutant Escherichia coli . New England Journal of Medicine 307:1225–1228
    [Google Scholar]
  32. Zinner S H, Peter G. 1983; The potential role of cell wall core glycoplipids in the immunoprophylaxis and therapy of gram-negative rod bacteraemia. Easmon C S F, Jeljaszewicz J. Medical Microbiology II Academic Press; London:71–85
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-2-85
Loading
/content/journal/jmm/10.1099/00222615-25-2-85
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error