1887

Journal of Medical Microbiology logo

Volume 25, Issue 2

Protective capacity of antibodies to outer-membrane components of in a systemic mouse peritonitis model Free

Abstract

Summary

Antibody-mediated protection was studied in an experimental murine model of peritonitis-septicaemia with O18: K1. Protection from lethal intraperitoneal challenge was achieved by passive immunisation with horse anti-K1 capsular antiserum (H46) or rabbit antiserum to the homologous O18 antigen. The maximum increase in LD50 achieved with anti-K1 and anti-O18 antibodies was 10-and 5-fold, respectively. The protective capacity of the anti-O serum was found to be in the IgG fraction. Rabbits were also immunised with various semi-purified or purified outer-membrane-protein preparations (porins and OmpA protein) from rough or strains or with whole J5 bacteria. Although this immunisation resulted in high antibody titres to homologous and, to a lesser extent, also to heterologous antigens, none of the antisera protected against challenge with the capsulate O 18: K1 bacteria.

  • Received:
  • Accepted:
  • Published Online:
© 1988 The Pathological Society of Great Britain and Ireland
Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-2-77
1988-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/2/medmicro-25-2-77.html?itemId=/content/journal/jmm/10.1099/00222615-25-2-77&mimeType=html&fmt=ahah

References

  1. Allen P Z V, Glode M, Schneerson R, Robbins J B. 1982; Identification of immunoglobulin heavy-chain isotypes of specific antibodies of horse 46 group B meningococcal antiserum. Journal of Clinical Microbiology 15:324–329
     [Google Scholar]
  2. Bortolussi R, Ferrieri P. 1980; Protection against Escherichia coli K1 infection in newborn rats by antibody to K1 capsular polysaccharide antigen. Infection and Immunity 28:111–117
     [Google Scholar]
  3. Braude A I, Ziegler E J, Douglas H, McCutchan J A. 1977; Antibody to cell wall glycolipid of gram-negative bacteria : Induction of immunity to bacteremia and endotoxemia. Journal of Infectious Diseases 136:S167–S173
     [Google Scholar]
  4. Braun V, Bosch V, Klumpp E R, Neff I, Mayer H, Schlecht S. 1976; Antigenic determinants of murein lipoprotein and its exposure at the surface of Enterobacteriaceae. European Journal of Biochemistry 62:555–566
     [Google Scholar]
  5. Cross A S, Zollinger W, Mandrell R, Gemski P, Sadoff J. 1983; Evaluation of immunotherapeutic approaches for the potential treatment of infections caused by K1-positive Escherichia coli . Journal of Infectious Diseases 147:68–76
     [Google Scholar]
  6. de Jongh-Leuvenink J, Vreede R W, Marcelis J H, de Vos M, Verhoef J. 1985; Detection of antibodies against lipopolysac-charides of Escherichia coli and Salmonella R and S strains by immuno-blotting. Infection and Immunity 50:716–720
     [Google Scholar]
  7. Elbein A D, Heath E C. 1965; The biosynthesis of cell wall lipopolysaccharide in Escherichia coli I. The biochemical properties of a uridine diphosphate galactose-4-epimerase-less mutant. Journal of Biological Chemistry 240:1919–1925
     [Google Scholar]
  8. Engvall E, Perlmann P. 1972; Enzyme-linked immunosorbent assay ELISA. III. Quantitation of specific antibodies by enzyme labeled anti-immunoglobulin in antigen coated tubes. Journal of Immunology 109:129–135
     [Google Scholar]
  9. Galanos C, Luderitz O, Westphal O. 1969; A new method for the extraction of R lipopolysaccharides. European Journal of Biochemistry 9:245–249
     [Google Scholar]
  10. Gilleland H E, Parker M G, Matthews J M, Berg R D. 1984; Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice. Infection and Immunity 44:49–54
     [Google Scholar]
  11. Greisman S E, DuBuy J B, Woodward C L. 1978; Experimental gram-negative bacterial sepsis : Re-evaluation of the ability of rough mutant antisera to protect mice. Proceedings of the Society for Experimental Biology and Medicine 158:482–490
     [Google Scholar]
  12. Gross R J, Cheasty T, Rowe B. 1977; Isolation of bacteriophages specific for the K1 polysaccharide antigen of Escherichia coli . Journal of Clinical Microbiology 6:548–550
     [Google Scholar]
  13. Hofstra H, Dankert J. 1980; Major outer membrane proteins: Common antigens in Enterobacteriaceae species. Journal of General Microbiology 119:123–131
     [Google Scholar]
  14. Horwitz M A, Silverstein S C. 1980; Influence of the Escherichia coli capsule on complement fixation and on phagocytosis and killing by human phagocytes. Journal of Clinical Investigation 65:82–94
     [Google Scholar]
  15. Kaijser B, Holmgren J, Hanson L Å. 1972; The protective effect against E. coli of O and K antibodies of different immunoglobulin classes. Scandinavian Journal of Immunology 1:27–32
     [Google Scholar]
  16. Kallio P, Simonen M, Palva I, Sarvas M. 1986; Synthesis of OmpA protein of Escherichia coli K12 in Bacillus subtilis . Journal of General Microbiology 132:677–687
     [Google Scholar]
  17. Kasper D L, Winkelhake J L, Zollinger W D, Brandt BL, Artenstein M S. 1973; Immunochemical similarity between polysaccharide antigens of Escherichia coli O7 : K1(L):NM and group B Neisseria meningitidis . Journal of Immunology 110:262–268
     [Google Scholar]
  18. Kauffmann F. 1966 The bacteriology of Enterobacteriaceae ; collected studies, 3. Munksgaard; Copenhagen:
     [Google Scholar]
  19. Kim K S, Cross A S, Zollinger W, Sadoff J. 1985; Prevention and therapy of experimental Escherichia coli infection with monoclonal antibody. Infection and Immunity 50:734–737
     [Google Scholar]
  20. Kuusi N, Nurminen M, Saxén H, Valtonen M, Mäkelä P H. 1979; Immunization with major outer membrane proteins in experimental salmonellosis of mice. Infection and Immunity 25:857–862
     [Google Scholar]
  21. Kuusi N, Nurminen M, Saxén H, Mäkelä P H. 1981; Immunization with major outer membrane protein (porin) preparations in experimental murine salmonellosis : effect of lipopolysaccharide. Infection and Immunity 34:328–332
     [Google Scholar]
  22. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 111:680–685
     [Google Scholar]
  23. Leinonen M. 1985; Serological methods for the study of bacterial surface antigens. Korhonen T. K. K. Enterobacterial surface antigens. Methods for molecular characterization Elsevier Science Publishers; Amsterdam:179–206
     [Google Scholar]
  24. Lowry O H, Rosebrough N J, Farr A L, Randall R J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  25. Mayer H, Rapin A M C, Schmidt G, Boman H G. 1976; Immunochemical studies on lipopolysaccharides from wild-type and mutants of Escherichia coli K-12. European Journal of Biochemistry 66:357–368
     [Google Scholar]
  26. Mäkelä P H, Stocker B A D. 1984; Genetics of lipopolysaccharide. Rietschel E. T. Handbook of endotoxin 1 Chemistry of endotoxin Elsevier Science Publishers; Amsterdam:59–137
     [Google Scholar]
  27. Nikaido H, Levinthal M, Nikaido K, Nakane K. 1967; Extended deletions in the histidine-rough-B region of the Salmonella chromosome. Proceedings of the National Academy of Sciences of the USA 57:1825–1832
     [Google Scholar]
  28. Nowicki B, Vuopio-Varkila J, Viljanen P, Korhonen T K, Mäkelä P H. 1986; Fimbrial phase variation and systemic Escherichia coli infection studied in the mouse peritonitis model. Microbial Pathogenesis 1:335–347
     [Google Scholar]
  29. Nurminen M. 1978; A mild procedure to isolate the 34K, 35K and 36K porins of the outer membrane of Salmonella typhimurium . FEMS Microbiology Letters 3:331–334
     [Google Scholar]
  30. Pluschke G, Achtman M. 1985; Antibodies to O-antigen of lipopolysaccharide are protective against neonatal infection with Escherichia coli K1. Infection and Immunity 49:365–370
     [Google Scholar]
  31. Reed L J, Muench H. 1938; A simple method of estimating fifty per cent end-points. American Journal of Hygiene 27:493–497
     [Google Scholar]
  32. Reif A E. 1969; Batch preparation of rabbit γG globulin with DEAE-cellulose. Immunochemistry 6:723–731
     [Google Scholar]
  33. Robbins J B. 1978; Vaccines for the prevention of encapsulated bacterial diseases : current status, problems and prospects for the future. Immunochemistry 15:839–854
     [Google Scholar]
  34. Saxén H, Mäkelä O. 1982; The protective capacity of immune sera in experimental mouse salmonellosis is mainly due to IgM antibodies. Immunology Letters 5:267–272
     [Google Scholar]
  35. Saxén H, Nurminen M, Kuusi N, Svenson S B, Mäkelä P H. 1986; Evidence for the importance of O antigen specific antibodies in mouse-protective Salmonella outer membrane protein (porin) antisera. Microbial Pathogenesis 1:433–442
     [Google Scholar]
  36. Siber G R, Kania S A, Warren H S. 1985; Cross-reactivity of rabbit antibodies to lipopolysaccharides of Escherichia coli J5 and other gram-negative bacteria. Journal of Infectious Diseases 152:954–964
     [Google Scholar]
  37. Speer C P, Hauptmann D, Stubbe P, Gahr M. 1985; Neonatal septicemia and meningitis in Göttingen, West Germany. Pediatric Infectious Diseases 4:36–41
     [Google Scholar]
  38. Teng N N H. 1985; Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies. Proceedings of the National Academy of Sciences of the USA 82:1790–1794
     [Google Scholar]
  39. Tsai C-M, Frasch C E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
     [Google Scholar]
  40. Van der Ley P, Kuipers O, Tommassen J, Lugtenberg B. 1986; O-antigenic chains of lipopolysaccharide prevent binding of antibody molecules to an outer membrane pore protein in Enterobacteriaceae. Microbial Pathogenesis 1:43–49
     [Google Scholar]
  41. Weinstein M P, Reller L B, Murphy J R, Lichtenstein K A. 1983; The clinical significance of positive blood cultures: a comprehensive analysis of 500 episodes of bacteremia and fungemia in adults. I. Laboratory and epidemiologie observations. Reviews of Infectious Diseases 5:35–53
     [Google Scholar]
  42. Welch W D, Martin W J, Stevens P, Young L S. 1979; Relative opsonic and protective activities of antibodies against K1, O and lipid A antigens of Escherichia coli . Scandinavian Journal of Infectious Diseases 11:291–301
     [Google Scholar]
  43. Westphal O, Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further application of the procedure. Methods in Carbohydrate Chemistry 5:83–91
     [Google Scholar]
  44. Wilkinson R G, Gemski P, Stocker B A D. 1972; Non-smooth mutants of Salmonella typhimurium : differentiation by phage sensitivity and genetic mapping. Journal of General Microbiology 70:527–554
     [Google Scholar]
  45. Ziegler E J, Douglas H, Sherman J E, Davis C E, Braude A I. 1973; Treatment of E. coli and Klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-GAL epimerase-deficient mutant. Journal of Immunology 111:433–438
     [Google Scholar]
  46. Ziegler E J, McCutchan J A, Fierer J, Glauser M P, Sadoff J C, Douglas H, Braude A I. 1982; Treatment of gram-negative bacteremia and shock with human antiserum to a mutant Escherichia coli . New England Journal of Medicine 307:1225–1230
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-2-77
Loading
Protective capacity of antibodies to outer-membrane components of Escherichia coli in a systemic mouse peritonitis model
J. Med. Microbiol. 25, 77 (1988); https://doi.org/10.1099/00222615-25-2-77
/content/journal/jmm/10.1099/00222615-25-2-77
/content/journal/jmm/10.1099/00222615-25-2-77
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error