1887

Abstract

Summary

Six strains of (W118, TML, SL 1027, LT7, M206 and Thax 1) of different virulence were examined for the presence of antigens which react with antibodies to cholera toxin (anti-CT). A fluorescent-antibody-labelling technique employing anti-CT was used to analyse antigen expression. A rapid increase in the proportion of cells producing a CT-related antigen was demonstrated in cells in early log phase (1–4 h growth) followed by a rapid decline during mid-late log phase in each of the six strains. The nature of the CT-related antigen was analysed by immunoblotting using anti-CT. An antigen of mol. wt equivalent to a high-mol. wt species of CT B subunit was detected in polymyxin-B extracts of all strains but greater amounts were observed in the strains that we consider avirulent. Nothing equivalent to a CT A-related subunit was observed in any of the strains. The relatedness of the salmonella antigen to CT was limited. The high-mol. wt antigen was not disrupted in the denaturing conditions of SDS-PAGE; nothing was detected by enzyme-linked immunosorbent assays with either ganglioside or anti-CT as anchor.

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1988-02-01
2022-01-20
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References

  1. Clements J D, Finkelstein R A. 1979; Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures. Infection and Immunity 24:760–769
    [Google Scholar]
  2. Finkelstein R A, Marchlewicz B A, McDonald R J, Boesman-Finkelstein M. 1983; Isolation and characterization of a cholera-related enterotoxin from Salmonella typhimurium . FEMS Microbiology Letters 17:239–241
    [Google Scholar]
  3. Giannella R A, Formal S B, Dammin G J, Collins H. 1973; Pathogenesis of salmonellosis. Studies of fluid secretion, mucosal invasion and morphological reaction in the rabbit ileum. Journal of Clinical Investigation 52:441–453
    [Google Scholar]
  4. Gill D M. 1976; The arrangement of subunits in cholera toxin. Biochemistry 15:1242–1248
    [Google Scholar]
  5. Holmgren J, Fredman P, Lindblad M, Svennerholm A-M, Svennerholm L. 1982; Rabbit intestinal glycoprotein receptor for Escherichia coli heat-labile enterotoxin lacking affinity for cholera toxin. Infection and Immunity 38:424–433
    [Google Scholar]
  6. Holmgren J, Lindblad M, Fredman P, Svennerholm L, Myrvold H. 1985; Comparison of receptors for cholera and Escherichia coli enterotoxins in human intestine. Gastroenterology 89:27–35
    [Google Scholar]
  7. Jiwa S F H. 1981; Probing for enterotoxigenicity among the salmonellae: an evaluation of biological assays. Journal of Clinical Microbiology 14:463–472
    [Google Scholar]
  8. Jiwa S F H, Månsson I. 1983; Hemagglutinating and hydrophobic surface properties of salmonellae producing enterotoxin neutralized by cholera antitoxin. Veterinary Microbiology 8:443–458
    [Google Scholar]
  9. Koupal L R, Deibel R H. 1975; Assay, characterization and localization of an enterotoxin produced by salmonella. Infection and Immunity 11:14–22
    [Google Scholar]
  10. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  11. O’Brien A D, LaVeck G D, Thompson M R, Formal S B. 1982; Production of Shigella dysenteriae type 1 -like cytotoxin by Escherichia coli . Journal of Infectious Diseases 146:763–769
    [Google Scholar]
  12. Peterson J W, Houston C W, Koo F C W. 1981; Influence of cultural conditions on mitomycin C-mediated bacteriophage induction and release of Salmonella toxin. Infection and Immunity 32:232–242
    [Google Scholar]
  13. Redmond S C, Ketley J M, Mitchell T J, Stephen J, Burdon D W, Candy D C A. 1985; Detection of Clostridium difficile enterotoxin. Collins C H, Grange J M. Isolation and identification of microorganisms of medical and veterinary importance; Society for Applied Bacteriology Technical Series No. 21 Academic Press; London:237–250
    [Google Scholar]
  14. Sandefur P D, Peterson J W. 1976; Isolation of skin permeability factors from culture filtrates of Salmonella typhimurium . Infection and Immunity 14:671–679
    [Google Scholar]
  15. Sandefur P D, Peterson J W. 1977; Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin. Infection and Immunity 15:988–992
    [Google Scholar]
  16. Sedlock D M, Deibel R H. 1978; Detection of Salmonella enterotoxin using rabbit ileal loops. Canadian Journal of Microbiology 24:268–273
    [Google Scholar]
  17. Stephen J, Wallis T S, Starkey W G, Candy D C A, Osborne M P, Haddon S. 1985Salmonellosis: in retrospect and prospect. In: Microbial toxins and diarrhoeal disease, Ciba Foundation Symposium No. 112. Pitman MedicalTunbridge Wells175–192
    [Google Scholar]
  18. Svennerholm A-M, Holmgren J. 1978; Identification of Escherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GMj ELISA) procedure. Current Microbiology 1:19–23
    [Google Scholar]
  19. Tsuji T, Honda T, Miwatani T. 1984; Comparison of effects of nicked and unnicked Escherichia coli heat-labile enterotoxin on Chinese hamster ovary cells. Infection and Immunity 46:94–97
    [Google Scholar]
  20. Wallis T S, Starkey W G, Stephen J, Haddon S J, Osborne M P, Candy D C A. 1986; Enterotoxin production by Salmonella typhimurium strains of different virulence. Journal of Medical Microbiology 21:19–23
    [Google Scholar]
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