1887

Abstract

Summary

Resistance to growth of at the mucosa of blind intestinal loops developed in rats after intestinal or parenteral exposure to live organisms or other antigenic materials. Simultaneous serological studies suggested that neither serum vibriocidal activity nor intestinal mucus antibodies are likely to provide a direct test of antibacterial immune status. Challenge of rats 4 weeks after one dose of antigen may reveal a form of immunity that is not related to antibodies in the intestine and possibly is analogous to long-term immunity expressed in man following infection with the organism. This immunity has not been attributed to lipopolysaccharide (LPS) antigen and does not appear to involve flagella-associated antigens ; involvement of antigens other than LPS, such as protein antigens of the outer membranes of , has not yet been substantiated. Separation of monomeric sub-units of outer-membrane proteins by hydrophobic interaction high pressure liquid chromatography has revealed significant quantitative differences among preparations derived from the common serotypes of the organism. These differences may be sufficient to explain the better protection observed when homologous serotypes were used for immunisation and challenge in the long-term resistance model.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-2-129
1988-02-01
2023-02-08
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/2/medmicro-25-2-129.html?itemId=/content/journal/jmm/10.1099/00222615-25-2-129&mimeType=html&fmt=ahah

References

  1. Attridge S R, Rowley D. 1983; Prophylactic significance of the nonlipolysaccharide antigens of Vibrio cholerae . Journal of Infectious Diseases 148:931–939
    [Google Scholar]
  2. Auzins I. 1968; A comparative assay of O-somatic antigen 5 of salmonellae. Australian Journal of Experimental Biology and Medical Science 46:93–105
    [Google Scholar]
  3. Cash R A, Music S I, Libonati J P, Craig J P, Pierce N F, Hornick R B. 1974; Response of man to infection with Vibrio cholerae. II Protection from illness afforded by previous disease and vaccine. Journal of Infectious Diseases 130:325–333
    [Google Scholar]
  4. Clements M L. 1982; Magnitude, kinetics and duration of vibriocidal antibody responses in North Americans after ingestion of Vibrio cholerae . Journal of Infectious Diseases 145:465–473
    [Google Scholar]
  5. Cooper G N, Jackson G D F. 1981; Immune responses of rats to live Vibrio cholerae: antibodies in serum and intestinal secretions. Parasite Immunology 3:57–68
    [Google Scholar]
  6. Cooper G N, McNab C E, Walker P G, Jackson G D F. 1984; Intestinal antibodies in rats following exposure to live Vibrio cholerae . Australian Journal of Experimental Biology and Medical Science 62:465–477
    [Google Scholar]
  7. Cooper G N, Narendranathan R. 1986; Antibacterial immunity to Vibrio cholerae in rats. Journal of Medical Microbiology 22:133–141
    [Google Scholar]
  8. Eubanks E R, Guentzel M N, Berry L J. 1977; Evaluation of surface components of Vibrio cholerae as protective iramu- nogens. Infection and Immunity 15:533–538
    [Google Scholar]
  9. Hawkes R, Niday E, Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  10. Holmgren J, Svennerholm A-M. 1983; Cholera and the immune response. Progress in Allergy 33:106–119
    [Google Scholar]
  11. Kabir S. 1983; The serological properties of the cell surface proteins of Vibrio cholerae . Journal of General Microbiology 129:2199–2206
    [Google Scholar]
  12. Laemmli U K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  13. Levine M M. 1979; Immunity of cholera in man: relative role of antibacterial versus antitoxic immunity. Transactions of the Society of Tropical Medicine and Hygiene 73:3–9
    [Google Scholar]
  14. Mosley W H, Aziz K M A, Rahman A S M M, Chowdhury A K M A, Ahmed A. 1973; Field trials of monovalent Ogawa and Inaba cholera vaccines in rural Bangladesh : three years of observation. Bulletin of the World Health Organization 49:381–387
    [Google Scholar]
  15. Schaedler R W, Dubos R, Costello R. 1965; The development of the bacterial flora in the gastrointestinal tract of mice. Journal of Experimental Medicine 122:59–66
    [Google Scholar]
  16. Schnaitman C A. 1971; Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. Journal of Bacteriology 108:545–552
    [Google Scholar]
  17. Sears S D, Richardson K, Young C, Parker C D, Levine M M. 1984; Evaluation of the human immune response to outer membrane proteins of Vibrio cholerae . Infection and Immunity 44:439–444
    [Google Scholar]
  18. Spira W M, Sack R B, Froehlich J L. 1981; Simple adult rabbit model for Vibrio cholerae and enterotoxigenic Escherichia coli diarrhea. Infection and Immunity 32:739–747
    [Google Scholar]
  19. Svennerholm A-M, Holmgren J, Ouchterlony O. 1975; Experimental studies on cholera immunisation. V. Cross reactive and type specific determinants on V. cholerae Inaba and Ogawa endotoxin and their significance for protective immunity. Acta Pathologica et Microbiologica Scandinavica Section C 83:221–230
    [Google Scholar]
  20. Tokunaga E, Cray W C, Pierce N F. 1984; Compared colonizing and immunizing efficiency of toxinogenic (A+ B+) Vibrio cholerae and an AB+ mutant (Texas Star-SR) studied in adult rabbits. Infection and Immunity 44:364–369
    [Google Scholar]
  21. Towbin H, Staehelin T, Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences of the USA 76:4350–4354
    [Google Scholar]
  22. Ujiiye A, Kobari K. 1970; Protective effect on infections with Vibrio cholerae in suckling mice caused by the passive immunization with milk of immune mothers. Journal of Infectious Diseases 121:s50–s55
    [Google Scholar]
  23. Westphal O, Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure Whistler R L. Methods in carbohydrate chemistry Academic Press Inc; New York: 583–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-2-129
Loading
/content/journal/jmm/10.1099/00222615-25-2-129
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error