1887

Abstract

Summary

The plasmid profiles of 112 clinical isolates of were determined by two reproducible, rapid, plasmid screening methods. Plasmid DNA was present in 15% of isolates examined. Plasmids varied in size from 1·2 – 10 to 60·2 – 10 mol. wt. The dominant serotypes encountered were O:11 and O:4, which comprised 38% and 12% of strains, respectively. Four pyocin types (1, 10, 3 and 5) dominated (respective frequencies: 56, 15, 12 and 6%). Reproducibility of pyocin typing was distinctly inferior to both plasmid profiling and serotyping. Strains of identical serotypes could be further differentiated by dissimilar plasmid profiles. Serologically untypable or polyagglutinable strains were successfully characterised by plasmid profile patterns. Thus, plasmid profiling was shown to be a useful adjunct to serotyping for the epidemiological typing of . .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-2-109
1988-02-01
2022-12-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/2/medmicro-25-2-109.html?itemId=/content/journal/jmm/10.1099/00222615-25-2-109&mimeType=html&fmt=ahah

References

  1. Acar J F. 1980; The susceptibility test. Lorian V. Antibiotics in laboratory medicine Williams and Williams; Baltimore:24–54
    [Google Scholar]
  2. Archer G L, Mayhall C G. 1983; Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections. Journal of Clinical Microbiology 18:395–399
    [Google Scholar]
  3. Bauer A W, Kirby W M M, Sherris J C, Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45:493–496
    [Google Scholar]
  4. Bergan T. 1975; Epidemiological typing of Pseudomonas aeruginosa . Brown M R W. Resistance of Pseudomonas aeruginosa John Wiley; New York:189–235
    [Google Scholar]
  5. Bergan T. 1978; Phage typing of Pseudomonas aeruginosa . Bergan T, Norris J R. Methods in microbiology 10 Academic Press Inc; London:169–199
    [Google Scholar]
  6. Bezanson G S, Khakhria R, Pagnutti D. 1983; Plasmid profiles of value in differentiating Salmonella muenster isolates. Journal of Clinical Microbiology 17:1159–1160
    [Google Scholar]
  7. Bodey G P, Bolivar R, Fainstein V, Jadeja L. 1983; Infections caused by Pseudomonas aeruginosa . Reviews of Infectious Diseases 5:279–313
    [Google Scholar]
  8. Bradbury W C, Marko M A, Hennessy J N, Penner J L. 1983; Occurrence of plasmid DNA in serologically defined strains of Campylobacter jejuni and Campylobacter coli . Infection and Immunity 40:460–463
    [Google Scholar]
  9. Christensen G D, Parisi J T, Bisno A L, Simpson W A, Beachey E H. 1983; Characterization of clinically significant strains of coagulase-negative staphylococci. Journal of Clinical Microbiology 18:258–269
    [Google Scholar]
  10. Fyfe J A, Harris G, Govan J R W. 1984; Revised pyocin typing method for Pseudomonas aeruginosa . Journal of Clinical Microbiology 20:47–50
    [Google Scholar]
  11. Gillies R R, Govan J R W. 1966; Typing of Pseudomonas pyocyanea by pyocine production. Journal of Pathology and Bacteriology 91:339–345
    [Google Scholar]
  12. Govan J R W. 1978; Pyocin typing of Pseudomonas aeruginosa . Bergan T, Norris J R. Methods in microbiology 10 Academic Press Inc; London:61–91
    [Google Scholar]
  13. Holmberg S D, Wachsmuth I K, Hickman-Brenner F W, Cohen M L. 1984; Comparison of plasmid profile analysis, phage typing and antimicrobial susceptibility testing in characterizing Salmonella typhimurium isolates from outbreaks. Journal of Clinical Microbiology 19:100–104
    [Google Scholar]
  14. Kado C I, Liu S. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  15. Lanyi B, Bergan T. 1978; Serological characterization of Pseudomonas aeruginosa . Bergan T, Norris J R. Methods in microbiology 10 Academic Press Inc; London:93–168
    [Google Scholar]
  16. Liu P V, Matsumoto H, Kusama H, Bergan T. 1983; Survey of heat-stable, major somatic antigens of Pseudomonas aeruginosa . International Journal of Systematic Bacteriology 33:256–264
    [Google Scholar]
  17. Maher W E, Plouffe J F, Para M F. 1983; Plasmid profiles of clinical and environmental isolates of Legionella pneumo-phila serogroup 1. Journal of Clinical Microbiology 18:1422–1423
    [Google Scholar]
  18. Pitt T L. 1981; Preparation of agglutinating antisera specific for the flagellar antigens of Pseudomonas aeruginosa . Journal of Medical Microbiology 14:251–260
    [Google Scholar]
  19. Portnoy D A, Moseley S L, Falkow S. 1981; Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis. Infection and Immunity 31:775–782
    [Google Scholar]
  20. Schaberg D R, Tompkins L S, Falkow S. 1981; Use of agarose gel electrophoresis of plasmid deoxyribonucleic acid to fingerprint gram-negative bacilli. Journal of Clinical Microbiology 13:1105–1108
    [Google Scholar]
  21. Tagg J R, Mushin R. 1971; Epidemiology of Pseudomonas aeruginosa infection in hospitals. 1. Pyocin typing of Ps aeruginosa . Medical Journal of Australia 1:847–852
    [Google Scholar]
  22. Taylor D N. 1982; Salmonellosis associated with marijuana: a multistate outbreak traced by plasmid fingerprinting. New England Journal of Medicine 306:1249–1253
    [Google Scholar]
  23. Tompkins L S, Plorde J J, Falkow S. 1980; Molecular analysis of R-factors from multiresistant nosocomial isolates. Journal of Infectious Diseases 141:625–636
    [Google Scholar]
  24. Williams R J, Govan J R W. 1973; Pyocine typing of mucoid strains of Pseudomonas aeruginosa isolated from children with cystic fibrosis. Journal of Medical Microbiology 6:409–412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-2-109
Loading
/content/journal/jmm/10.1099/00222615-25-2-109
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error