1887

Abstract

Summary

Lysosomotropic weak bases impair in-vitro neutrophil functions including intracellular killing of strain 502a. To investigate whether prevention of phagosomal acidification could account for impaired microbicidal activity, a model phagosome was formulated with a freeze-thawed granule extract as a source of lysosomal enzymes and HO as a source of toxic oxygen metabolites. The lysosomal extract alone killed strain S15 efficiently at H 5·5 and 7·0, but had little activity against . 502a. Sublethal concentrations of the two agents, when combined, acted synergically against either organism. Each organism was killed more effectively at H 5·5 than at H 7·0 by the lysosome extract-HO combination, but the killing of . was more rapid than that of . in the same conditions. These findings suggest that impairment of neutrophil antistaphylococcal activity by weak bases may be mediated by their ability to raise phagosomal H, and that persistence of . in similar conditions does not occur because the latter is killed by lysosomal constituents in a non-H-dependent fashion.

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1988-02-01
2022-09-25
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