1887

Abstract

Summary

A collection of 198 clinical isolates of strains belonging to the tribe was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of , and . Approximately half of the . strains were haemolytic on blood agar. This activity was not associated with any particular bacteriocin type. The haemolysin was also produced during exponential growth in broth and was thermolabile and calcium dependent. All . strains and some . strains were non-haemolytic on blood agar. However, most strains of the spp., irrespective of their bacteriocin and antigenic type, produced, over a short period during exponential growth in broth, a heat-stable, cell-associated calcium-independent haemolysin. A smaller proportion of . and . strains produced, in addition, a thermolabile, calcium-dependent haemolysin which was associated with the formation of large haemolytic zones on blood agar. The relationship of these haemolysins to haemolysin and their possible role in virulence is discussed. Haemolysin production was not found in any of the 74 strains of four species of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-25-1-17
1988-01-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/25/1/medmicro-25-1-17.html?itemId=/content/journal/jmm/10.1099/00222615-25-1-17&mimeType=html&fmt=ahah

References

  1. Bamforth J, Dudgeon J A. 1952; The haemolytic activity of Bact. coli . Journal of Pathology and Bacteriology 64:751–761
    [Google Scholar]
  2. Bergström T. 1972; Sex differences in childhood urinary tract infection. Archives of Diseases of Childhood 47:227–232
    [Google Scholar]
  3. Brooks H J L, O’Grady F, McSherry M A, Cattell W R. 1980; Uropathogenic properties of Escherichia coli in recurrenturinary-tract infection. Journal of Medical Microbiology 13:57–68
    [Google Scholar]
  4. Cooke E M, Ewins S P. 1975; Properties of strains of Escherichia coli isolated from a variety of sources. Journal of Medical Microbiology 8:107–111
    [Google Scholar]
  5. Dudgeon L S, Wordley E, Bawtree F. 1921; On Bacillus coliinfections of the urinary tract, especially in relation tohaemolytic organisms. Journal of Hygiene 20:137–164
    [Google Scholar]
  6. Emödy L, Vörös S, Pál T. 1982; Alpha-haemolysin, a possiblevirulence factor in Proteus morganii . FEMS Microbiology Letters 13:329–331
    [Google Scholar]
  7. Green C P, Thomas V L. 1981; Hemagglutination of human type O erythrocytes, hemolysin production, and serogrouping of Escherichia coli isolates from patients with acute pyelonephritis, cystitis, and asymptomatic bacteriuria. Infection and Immunity 31:309–315
    [Google Scholar]
  8. Hacker J, Schroter G, Schrettenbrunner A, Hughes C, Goebel W. 1983a; Hemolytic Escherichia coli strains in the human fecal flora as potential urinary pathogens. Zentralblatt för Bakteriologie . Mikrobiologie und Hygiene, 1 Abteilung Origi-naleA 254:370–378
    [Google Scholar]
  9. Hacker J, Hughes C, Hof H, Goebel W. 1983b; Cloned hemolysin genes from Escherichia coli that cause urinary tract infection determine different levels of toxicity in mice. Infection and Immunity 42:57–63
    [Google Scholar]
  10. Hacker J, Hughes C. 1985; Genetics of Escherichia coli hemolysin. Current Topics in Microbiology and Immunology 118:138–162
    [Google Scholar]
  11. Hallett R J, Pead L, Maskell R. 1976; Urinary infection in boys. A three year prospective study. Lancet 2:1107–1110
    [Google Scholar]
  12. Hickman F W, Steigerwalt A G, Farmer J J, Brenner D J. 1982; Identification of Proteus penned sp. nov. formerly known as Proteus vulgaris indole negative or as Proteus vulgaris biogroup 1. Journal of Clinical Microbiology 15:1097–1102
    [Google Scholar]
  13. Hickman-Brenner F W, Farmer J J, Steigerwalt A G, Brenner D J. 1983; Providencia rustigianii: a new species in the family Enterobacteriaceae formerly known as Providencia alcali-faciens biogroup 3. Journal of Clinical Microbiology 17:1057–1060
    [Google Scholar]
  14. Hughes C, Hacker J, Roberts A, Goebel W. 1983; Hemolysin production as a virulence marker in symptomatic and asymptomatic urinary tract infections caused by Escherichia coli . Infection and Immunity 39:546–551
    [Google Scholar]
  15. Jörgensen S E, Short E C, Kurtz H J, Mussen H K, Wu G K. 1976; Studies on the origin of the α-haemolysin produced by Escherichia coli . Journal of Medical Microbiology 9:173–189
    [Google Scholar]
  16. Khan A J, Ubriani R S, Bombach E, Agbayani M M, Ratner H, Evans H E. 1978; Initial urinary tract infection caused by Proteus mirabilis in infancy and childhood. Journal of Pediatrics 93:791–793
    [Google Scholar]
  17. Koronakis V, Cross M, Senior B, Koronakis E, Hughes C. 1987; The secreted hemolysins of Proteus mirabilis, Proteus vulgaris and Morganella morganii are genetically related to each other and to the alpha-hemolysin of Escherichia coli . Journal of Bacteriology 169:1509–1515
    [Google Scholar]
  18. Kotelko K, Kaca W, Rozalski A, Deka M. 1983; Some biological features of Proteus Bacilli. 2. Haemolytic activities of Proteus mirabilis and Proteus vulgaris strains. Acta Micro-biologica Polonica 32:345–351
    [Google Scholar]
  19. Larsson P, Oiling S. 1977; O antigen distribution and sensitivity to the bactericidal effect of normal human serum of Proteus strains from clinical specimens. Medical Microbiology and Immunology 163:77–82
    [Google Scholar]
  20. Lovell R, Rees T A. 1960; A filterable haemolysin from Escherichia coli . Nature 188:755–756
    [Google Scholar]
  21. Peerbooms P G H, Verweij A M J J, MacLaren D M. 1983; Investigation of the haemolytic activity of Proteus mirabilis strains. Antonie van Leeuwenhoek 49:1–11
    [Google Scholar]
  22. Peerbooms P G H, Verweij A M J J, MacLaren D M. 1984; Vero cell invasiveness of Proteus mirabilis . Infection and Immunity 43:1068–1071
    [Google Scholar]
  23. Rauss K F. 1936; The systematic position of Morgan’s Bacillus. Journal of Pathology and Bacteriology 42:183–192
    [Google Scholar]
  24. Rennie R P, Arbuthnott J P. 1974; Partial characterisation of Escherichia coli haemolysin. Journal of Medical Microbiology 7:179–188
    [Google Scholar]
  25. Rennie R P, Freer J H, Arbuthnott J P. 1974; The kinetics of erythrocyte lysis by Escherichia coli haemolysin. Journal of Medical Microbiology 7:189–195
    [Google Scholar]
  26. Senior B W. 1977; Typing of Proteus strains by proticineproduction and sensitivity. Journal of Medical Microbiology 10:7–17
    [Google Scholar]
  27. Senior B W. 1979; The special affinity of particular types ofProteus mirabilis for the urinary tract. Journal of Medical Microbiology 12:1–8
    [Google Scholar]
  28. Senior B W. 1983; Proteus morgani is less frequently associatedwith urinary tract infections than Proteus mirabilis—anexplanation. Journal of Medical Microbiology 16:317–322
    [Google Scholar]
  29. Senior B W. 1987; The typing of Morganella morgani by bacteriocinproduction and sensitivity. Journal of Medical Microbiology 23:33–39
    [Google Scholar]
  30. Senior B W, Larsson P. 1983; A highly discriminatory multi-typing scheme for Proteus mirabilis and Proteus vulgaris . Journal of Medical Microbiology 16:193–202
    [Google Scholar]
  31. Senior B W, Leslie D L. 1986; Rare occurrence of Proteus vulgarisin faeces: a reason for its rare association with urinary tractinfections. Journal of Medical Microbiology 21:139–144
    [Google Scholar]
  32. Short E C, Kurtz H J. 1971; Properties of the hemolytic activitiesof Escherichia coli . Infection and Immunity 3:678–687
    [Google Scholar]
  33. Smith H W. 1963; The haemolysins of Escherichia coli . Journal of Pathology and Bacteriology 85:197–211
    [Google Scholar]
  34. Snyder I S, Koch N A. 1966; Production and characteristics of hemolysins of Escherichia coli . Journal of Bacteriology 91:763–767
    [Google Scholar]
  35. Snyder I S, Zwadyk P. 1969; Some factors affecting productionand assay of Escherichia coli haemolysins. Journal of General Microbiology 55:139–143
    [Google Scholar]
  36. Taylor J F. 1928; B proteus infections. Journal of Pathology and Bacteriology 31:897–915
    [Google Scholar]
  37. Welch R A, Dellinger E P, Minshew B, Falkow S. 1981; Haemolysin contributes to virulence of extra-intestinal E coli infections. Nature 294:665–667
    [Google Scholar]
  38. Wenner J J, Rettger L F. 1919; Systematic study of the Proteus group of bacteria. Journal of Bacteriology 4:331–353
    [Google Scholar]
  39. Yacob M. 1932; Studies of the genus Proteus . Indian Journal of Medical Research 19:787–829
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-25-1-17
Loading
/content/journal/jmm/10.1099/00222615-25-1-17
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error