1887

Abstract

Summary.

Methicillin-sensitive and methicillin-resistant strains of from diverse geographic origins were analysed by polyacrylamide-agarose gel electrophoresis for esterase polymorphism. Three kinds of esterase bands, designated A, B and C, were defined by their ranges of activity toward five synthetic substrates and their resistance to di-isopropyl fluorophosphate. There were five allozymes of esterase A, four of esterase B and four of esterase C. Eighteen distinct combinations of allozymes (zymotypes) were distinguished amongst 105 strains analysed. Two major zymotypes were represented by 35 and 19 strains respectively, whereas other zymotypes were represented by one or, at most, seven strains. The coefficient of genetic diversity was lower for methicillin-resistant strains than for methicillin-sensitive strains. Most of the methicillin-resistant strains are represented by the two major zymotypes which differed from each other by the electrophoretic behaviour of the three esterases. These results indicate that, on the basis of esterase electrophoretic polymorphism, methicillin resistance is expressed in genetically different strains.

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1987-11-01
2022-01-22
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References

  1. Achtman M. 1986; Clonai analysis of Escherichia coli O2: Kl isolated from diseased humans and animals. Infection and Immunity 51:268–276
    [Google Scholar]
  2. Crossley K, Landesman B, Zaske D. 1979; An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. II. Epidemiologie studies. Journal of Infectious Diseases 139:280–287
    [Google Scholar]
  3. El Solh N, Fouace J M, Pillet J, Chabbert Y A. 1981; Plasmid DNA content of multiresistant Staphylococcus aureus strains. Annales de Microbiologie (Institut Pasteur) 132B:131–156
    [Google Scholar]
  4. Goullet P. 1973; An esterase zymogram of Escherichia coli. Journal of General Microbiology IT27–35
    [Google Scholar]
  5. Goullet P, Picard B. 1984; Distinctive electrophoretic and isoelectric focusing patterns of esterases from Yersinia enterocolitica and Yersinia pseudotuberculosis. Journal of General Microbiology 130:1471–1480
    [Google Scholar]
  6. Goullet P, Picard B. 1986a; Comparative esterase electrophoretic polymorphism of Escherichia coli isolates obtained from animal and human sources. Journal of General Microbiology 132:1843–1851
    [Google Scholar]
  7. Goullet P, Picard B. 1986b; Highly pathogenic strains of Escherichia coli revealed by the distinct electrophoretic patterns of carboxylesterase B. Journal of General Microbiology 132:1853–1858
    [Google Scholar]
  8. Kayser F H, Berger-Bachi B, Beck W D. 1986; Genetics of multiply resistant Staphylococcus aureus. Journal of Hospital Infection 7: Suppl A 19–27
    [Google Scholar]
  9. Kuhl S A, Pattee P A, Baldwin J N. 1978; Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus. Journal of Bacteriology 135:460–465
    [Google Scholar]
  10. Lacey R W, Grinsted J. 1973; Genetic analysis of methicillin-resistant strains of Staphylococcus aureus : evidence for their evolution from a single clone. Journal of Medical Microbiology 6:511–526
    [Google Scholar]
  11. Nei M. 1978; Estimation of average heterozygosity and genetic distance from a small sample of individuals. Genetics 89:583–590
    [Google Scholar]
  12. Schleifer K H, Schumacher-Perdreau F, Götz F, Popp B, Hajek V. 1976; Chemical and biochemical studies for the differentiation of coagulase-positive staphylococci. Archives of Microbiology 110:263–270
    [Google Scholar]
  13. Selander R K, Caugant D A, Ochman H, Musser J M, Gilmour M N, Whittam T S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and sys-tematics. Applied and Environmental Microbiology 51:873–884
    [Google Scholar]
  14. Sjöström J E, Löfdahl S, Philipson L. 1975; Transformation reveals a chromosomal locus of the gene(s) for methicillin resistance in Staphylococcus aureus. Journal of Bacteriology 123:905–915
    [Google Scholar]
  15. Townsend D E, Ashdown N, Annear D I, Pearman J W, Grubb W B. 1984; Genetic analysis of methicillin-resistant Staphylococcus aureus from a western Australian hospital. Journal of Hospital Infection 5:417–424
    [Google Scholar]
  16. Zimmerman R J, Kloos W E. 1976; Comparative zone electro-phoresis of esterases of Staphylococcus species isolated from mammalian skin. Canadian Journal of Microbiology 22:771–779
    [Google Scholar]
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