A procedure was developed for enumeration of total associated, attached and intracellular bacteria after interaction of spp. with epithelial cells Isogenic cultures of grown at 25°C had greater affinity for epithelial cells (Henle, HeLa and Vero) than for polystyrene, and they invaded the cells. and showed less attachment to either surface and were non-invasive. The degree of attachment to cells and invasion by was related to number of bacteria added and interaction time, whereas attachment to polystyrene occurred rapidly and did not change. was more hydrophobic when grown at 35°C than at 25°C according to partitioning in a biphasic dextran-polyethylene glycol system, and attached strongly to both polystyrene and epithelial-cell monolayers. grown at 25°C was also hydrophobic but did not have the same attachment properties. and showed slightly reduced electrostatic interactions with the anion exchangers DEAE-Sepharose and DEAE-Trisacryl. Attachment of to epithelial cells probably involves non-specific surface properties that are not entirely explicable by hydrophobic and electrostatic interactions, whereas invasion of epithelial cells appears to resemble “receptor-mediated endocytosis”.


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