@article{mbs:/content/journal/jmm/10.1099/00222615-24-2-157, author = "Ståhlberg, T. H. and Granfors, Kaisa and Toivanen, Auli", title = "Immunoblot analysis of human IgM, IgG and IgA responses to plasmid-encoded antigens of Yersinia enterocolitica serovar O3", journal= "Journal of Medical Microbiology", year = "1987", volume = "24", number = "2", pages = "157-163", doi = "https://doi.org/10.1099/00222615-24-2-157", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-24-2-157", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary. Human IgM, IgG and IgA responses after infection with Yersinia enterocolitica serovar O3 were studied by immunoblotting sera against whole-cell homogenates of a plasmid-containing strain of Y. enterocolitica O3 and a plasmid-free strain derived from it; each strain was grown in conditions expressive for the plasmid. The antibodies observed were directed against several plasmid-encoded polypeptides. The response against different bacterial components decreased uniformly with time and the persisting antibody production was directed against several epitopes. Strong reactions to the prominent plasmid-specified antigens of mol. wts (103) 26, 34, 45 and 52·5 were found more often with IgG-class antibodies than with IgM or IgA; the latter immunoglobulins recognised, respectively, antigens of mol. wt (103) 26 and 45 (IgM) and 26, 34 and 52·5 (IgA). Immunoblotting of sera from patients with yersinia-triggered reactive arthritis did not reveal any antigens that were involved additionally or specifically. However, IgA-mediated recognition of certain antigens of mol. wts (103) 26, 34 and 52·5 tended to persist longer in the arthritic patients.", }