@article{mbs:/content/journal/jmm/10.1099/00222615-24-2-145, author = "Sandin, R. L.", title = "Studies on cell adhesion and Concanavalin A-induced agglutination of Candida albicans after mannan extraction", journal= "Journal of Medical Microbiology", year = "1987", volume = "24", number = "2", pages = "145-150", doi = "https://doi.org/10.1099/00222615-24-2-145", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-24-2-145", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary. Candida albicans cells were treated with alkali and acid to extract preferentially the cell wall α-mannan. Cells were recovered at three stages, as extraction proceeded from mild to more extensive: Alk-1, Alk-2 and Alk + Acid. Yeast adhesion to human epithelial cells was then examined with an in-vitro adherence assay. Yeasts from all three stages of extraction adhered in significantly lower numbers to buccal mucosal cells than did unextracted yeasts. Adhesion was as low for Alk-1 cells as for those submitted to more complete mannan extraction. When yeast cells from all three stages were treated with Concanavalin A (Con A), a lectin probe with strong affinity for yeast α-mannans, and then subjected to the adherence assay, there was no significant change in adhesion. When yeast agglutinability by Con A was examined in tests with treated and untreated yeast cells, abundant agglutination occurred only with the untreated cells. However, Alk-1 cells, though lacking in adhesive capacity towards mucosal cells, showed significant agglutination. The results suggest that candidal adhesion is mediated by an alkali-soluble, mannan-containing moiety(ies) which appears to be lost early in the extraction process. Blockage of this moiety by Con A inhibits the adhesion of unextracted cells. Extracted cells lack this moiety but still possess enough structural mannan for Con A recognition and agglutination.", }