1887

Abstract

Summary.

Bacteroides antibodies were studied in sera from 74 patients infected with the fragilis group of and 74 healthy control persons, by immunofluorescence of 26 different serotypes of the fragilis group. Antibodies were present at titres of 10–320 in 65 (88%) patients and 50 (68%) controls (p < 0·01). Titres of ⩾80 were demonstrated in sera of 38 (51%) patients and 5 (7%) controls (p < 0·01). Specific IgM antibodies were detected in sera of 42 (57%) patients at a geometric mean titre (GMT) of 30, and 8(11%) controls at a GMT of 11 (p < 0·01). High antibody titres as well as specific IgM were found in 32 (43%) patients, while none of the controls showed such a combination (p < 0·01). The majority of positive patients’ sera (57%) reacted with five or more serotypes, whereas most positive control sera (51%) reacted against only one or two serotypes (p < 0·01). A selected combination of serotypes not reacting with the control sera showed positive reactions with 52 (70%) patients’ sera. These findings may be useful in devising schemes for the serodiagnosis of infection caused by the fragilis group of However, there are indications of geographic variation in prevalence of serotypes, which may prevent the development of a single universal scheme.

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1987-09-01
2023-02-01
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References

  1. Beckmann I, Meisel-Mikolajczyk F, Zuijderduijn J. 1979; Serologische Reaktivität von Gesunden, Kranken und Tumor-Kranken gegenüber antigenen verschiedener Bac-teroides-Serotypen. Zentralblatt für Bakteriologie Parasiten-kunde. Infektionskrankheiten und Hygiene, 1 Abteilung Originale B 168:452–462
    [Google Scholar]
  2. Danielsson D, Lambe D W, Persson S. 1974; Immune response to anaerobic infections. Balows A. Anaerobic bacteria : role in disease Charles C Thomas; Springfield IL:173–191
    [Google Scholar]
  3. Duerden B I, Collee J G, Brown R, Deacon A G, Holbrook W P. 1980; A scheme for the identification of clinical isolates of gram-negative anaerobic bacilli by conventional bacteriological tests. Journal of Medical Microbiology 13:231–245
    [Google Scholar]
  4. Elhag K M, Bettelheim K A, Tabaqchali S. 1977; Serological studies of Bacteroides fragilis. Journal of Hygiene 79:233–241
    [Google Scholar]
  5. Elhag K M, Tabaqchali S. 1978a; A study of the surface and somatic antigens of Bacteroides fragilis. Journal of Hygiene 80:439–449
    [Google Scholar]
  6. Elhag K M, Tabaqchali S. 1978b; The distribution of Bacteroides fragilis serotypes amongst clinical strains. Journal of Hygiene 81:89–97
    [Google Scholar]
  7. Elhag K M, Alwan M H, Al-Adnani M S, Sherif R A. 1986; Bacteroides fragilis is a silent pathogen in acute appendicitis. Journal of Medical Microbiology 21:245–249
    [Google Scholar]
  8. Gorbach S L, Bartlett J G. 1974; Anaerobic infections. New England Journal of Medicine 290:1177–1184
    [Google Scholar]
  9. Hofstad T. 1974; Antibodies reacting with lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis and Fusobacterium nucleatum in serum from normal human subjects. Journal of Infectious Diseases 129:349–352
    [Google Scholar]
  10. Holdeman L V, Cato E P, Moore W E C. 1977 Anaerobe laboratory manual, 4. Virginia Polytechnic Institute and State University; Blacksburg, VA:
    [Google Scholar]
  11. Isenberg H D, Washington J A, Balows A, Sonnenwirth A C. 1980; Collection, handling and processing of specimens. Lennette L. Manual of clinical microbiology, 3. American Society for Microbiology; Washington DC:52
    [Google Scholar]
  12. Lari J, Kirk D, Howden R. 1976; Bacteriological survey of acute appendicitis in children. British Journal of Surgery 63:643–646
    [Google Scholar]
  13. Leigh D A, Simmons K, Norman E. 1974; Bacterial flora of the appendix fossa in appendicitis and postoperative wound infection. Journal of Clinical Pathology 27:997–1000
    [Google Scholar]
  14. Moore W E C, Cato E P, Holdeman L V. 1969; Anaerobic bacteria of the gastrointestinal flora and their occurrence in clinical infections. Journal of Infectious Diseases 119:641–649
    [Google Scholar]
  15. Paavonen J, Valtonen V V, Kasper D L, Malkamäki M, Mäkelä P H. 1981; Serological evidence for the role of Bacteroides fragilis and Enterobacteriaceae in the pathogenesis of acute pelvic inflammatory disease. Lancet 1:293–295
    [Google Scholar]
  16. Polk B F, Kasper D L. 1977; Bacteroides fragilis subspecies in clinical isolates. Annals of Internal Medicine 86:569–571
    [Google Scholar]
  17. Quick J D, Goldberg H S, Sonnenwirth A C. 1972; Human antibody to Bacteroidaceae. American Journal of Clinical Nutrition 25:1351–1356
    [Google Scholar]
  18. Rissing J P, Buxton T B, Edmondson H T. 1979; Detection of specific IgG antibody in sera from patients infected with Bacteroides fragilis by Enzyme-Linked Immunosorbent Assay. Journal of Infectious Diseases 140:994–998
    [Google Scholar]
  19. Ross C A C, Gilmore R F. 1978; Serological diagnosis of Bacteroides fragilis infections by a complement fixation test. Journal of Clinical Pathology 31:1083–1084
    [Google Scholar]
  20. Wulff H, Lange J V. 1975; Indirect immunofluorescence for the diagnosis of Lassa fever infection. Bulletin of the World Health Organization 52:429–436
    [Google Scholar]
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