1887

Abstract

SUMMARY

Enteropathogenic strains are a common cause of infantile diarrhoea but do not produce recognised enterotoxins. Three strains of proven virulence were examined for toxins which may be missed in conventional tests. Cell lysates and concentrated culture supernates of organisms grown in five different media gave negative results when examined for adenylcyclase stimulating activity. The additions of zinc ions or lincomycin to these media or the use of iron-depleted media also gave negative results. The significance of these findings and the possible role of other toxins in diarrhoea due to enteropathogenic are discussed.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-23-4-335
1987-06-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/23/4/medmicro-23-4-335.html?itemId=/content/journal/jmm/10.1099/00222615-23-4-335&mimeType=html&fmt=ahah

References

  1. Cantey J. R. 1985; Shiga toxin—an expanding role in the pathogenesis of infectious disease. JournalofInfectiousDiseases 151:766–771
    [Google Scholar]
  2. Cleary T. G., Mathewson J. J., Faris E., Pickering L. K. 1985; Shiga-like cytotoxin production by enteropathogenic Escherichia coli serogroups. InfectionandImmunity 47:335–337
    [Google Scholar]
  3. Coulson C. J., Nassau P. M., Tait R. M. 1984; The ADP-ribosyltransferase activity of cholera toxin and Escherichia coli heat-labile toxin. BiochemicalSocietyTransactions 12:184–187
    [Google Scholar]
  4. Donohue-Rolfe A., Keusch G. T. 1983; Shigelladysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin B and osmotic shock. InfectionandImmunity 39:270–274
    [Google Scholar]
  5. Edelman R., Levine M. M. 1983; Summary of a workshop on enteropathogenic Escherichia coli. JournalofInfectiousDiseases 147:1108–1118
    [Google Scholar]
  6. Iwanaga M., Yamamoto K. 1985; New medium for the production of cholera toxin by Vibriocholera 01 biotype El Tor. Journal of Clinical Microbiology 22:405–408
    [Google Scholar]
  7. Kantor H. S., Tao P., Gorbach S. L. 1974; Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea. JournalofInfectiousDiseases 129:1–9
    [Google Scholar]
  8. Klipstein F. A., Rowe B., Engert R. F., Short H. B., Gross R. J. 1978; Enterotoxigenicity of enteropathogenic serotypes of Escherichia coli isolated from infants with epidemic diarrhea. InfectionandImmunity 21:171–178
    [Google Scholar]
  9. Levine M. M. 1978; Escherichia coli strains that cause diarrhoea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet 1:1119–1122
    [Google Scholar]
  10. Levine M. M. 1985; The diarrheal response of humans to some classic serotypes of enteropathogenic Escherichia coli is dependent on a plasmid encoding an enteroadhesiveness factor. Journal of Infectious Diseases 152:550–559
    [Google Scholar]
  11. Levner M., Wiener F. P., Rubin B. A. 1977; Induction of Escherichia coli and Vibriocholerae enterotoxins by an inhibitor of protein synthesis. InfectionandImmunity 15:132–137
    [Google Scholar]
  12. Long-Krug S. A., Weikel C. S., Tiemens K. T., Hewlett E. L., Levine M. M., Guerrant R. L. 1984; Does enteropathogenic Escherichia coli produce heat-labile enterotoxin, heat-stable enterotoxins a or b or cholera toxin A subunits?. Infection and Immunity 46:612–614
    [Google Scholar]
  13. Mundell D. H., Anselmo C. R., Wishnow R. M. 1976; Factors influencing heat-labile Escherichia coli enterotoxin activity. InfectionandImmunity 14:383–388
    [Google Scholar]
  14. Neville D. M. 1968; Isolation of an organ specific protein antigen from cell surface membrane of rat liver. Biochimica et Biophysica Acta 154:540–552
    [Google Scholar]
  15. O’Brien A. D., LeVeck G. D., Thompson M. R., Formal S. B. 1982; Production of Shigelladysenteriae type 1-like cytotoxin by Escherichia coli. JournalofInfectiousDiseases 146:763–769
    [Google Scholar]
  16. Robins-Browne R. M., Levine M. M., Rowe B., Gabriel E. M. 1982; Failure to detect conventional enterotoxins in classical enteropathogenic (serotyped) Escherichia coli strains of proven pathogenicity. InfectionandImmunity 38:798–801
    [Google Scholar]
  17. Salomon Y. 1979; Adenylate cyclase assay. In Brooker G., Greengard P., Robinson G. A. (eds) Advances in cyclic nucleotide research vol 10 Raven Press; New York: pp 3555
    [Google Scholar]
  18. Scheftel J. M., Martin C., Bober C., Monteil H. 1980; Isolation of an enterotoxic factor elaborated by human enteropathogenic Escherichia coli. FEMS Microbiology Letters 9:125–130
    [Google Scholar]
  19. Sugarman B., Epps L. R. 1984; Zinc and the heat-labile enterotoxin of Escherichia coli. Journal of Medical Microbiology 18:393–398
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-23-4-335
Loading
/content/journal/jmm/10.1099/00222615-23-4-335
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error