Fourteen recently isolated strains and two laboratory strains of were examined by electronmicroscopy for the presence of pili. All strains isolated recently from both men and women were heavily pilated. In contrast only a few pili were seen on organisms of the two laboratory strains, with many of the organisms having no pili. The importance of multiple subculture in this loss was supported by the observation that the degree of pilation of one freshly isolated strain decreased on repeated subculture. Other findings suggested that this was probably due to gradual loss of pili and not to selection of organisms that were non-pilate originally.


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