1887

Abstract

SUMMARY

The cell envelope protein patterns of 78 strains of , mainly isolated in hospitals, were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The patterns were stable and reproducible. Comparison of the protein profiles made possible differentiation between two groups of strains. The patterns of the first group could be classified on the basis of concordance. Some profiles appeared to be associated with the epidemiological origin of the strains. The second group consisted of strains with unique patterns which could not be classified. Comparison of SDS-PAGE patterns appears to be a suitable method for the relative classification of strains of nosocomial origin.

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1987-06-01
2022-01-23
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References

  1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. 1983; Six widespread bacterial clones among Escherichia coli K1 isolates. Infection and Immunity 39:315–335
    [Google Scholar]
  2. Alexander M., Ismail F., Jackman P. J. H., Noble W. C. 1984; Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic protein patterns. Journal of Medical Microbiology 18:55–64
    [Google Scholar]
  3. Barenkamp S. J., Munson R. S., Granoff D. M. 1981; Subtyping isolates of Haemophilus influenzae type b by outer-membrane protein profiles. Journal of Infectious Diseases 143:668–676
    [Google Scholar]
  4. Baumann P., Doudoroff M., Stanier R. Y. 1968; A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). Journal of Bacteriology 95:1520–1541
    [Google Scholar]
  5. Bouvet P. J. M., Grimont P. A. D. 1986; Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobac ter johnsonii sp. nov. and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter Iwoffii. International Journal of Systematic Bacteriology 36:228–240
    [Google Scholar]
  6. Brown M. R. W., Williams P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  7. Cowan S. T. 1974; Cowan and Steel’s Manual for the identification of medical bacteria. 2nd edn Cambridge University Press; Cambridge:
    [Google Scholar]
  8. Das B. C., Ayliffe G. A. J. 1984; Serotyping of Acinetobacter calcoaceticus.. Journal of Clinical Pathology 37:1388–1391
    [Google Scholar]
  9. Frasch C. E., Mocca L. F. 1982; Strains of Neisseria meningitidis isolated from patients and their close contacts. Infection and Immunity 37:155–159
    [Google Scholar]
  10. Glew R. H., Moellering R. C., Kunz L. J. 1977; Infections with Acinetobacter calcoaceticus (Herellea vaginicola): clinical and laboratory studies. Medicine (Baltimore) 56:79–97
    [Google Scholar]
  11. Henriksen S. D. 1973; Moraxella, Acinetobacter, and the Mimeae. Bacteriological Reviews 37:522–561
    [Google Scholar]
  12. Henriksen S. D. 1976; Moraxella, Neisseria, Branhamella, and Acinetobacter. Annual Review of Microbiology 30:63–83
    [Google Scholar]
  13. Holton J. 1982; A report of a further hospital outbreak caused by a multi-resistant Acinetobacter anitratus. Journal of Hospital Infection 3:305–909
    [Google Scholar]
  14. Hugh R. 1978; Classical methods for isolation and identification of glucose nonfermenting Gram-negative rods. In Gilardi G. L. (ed) Glucose nonfermenting Gram-negative bacteria in clinical microbiology CRC Press; West Palm Beach, FL: pp 3–6
    [Google Scholar]
  15. Juni E. 1984; Acinetobacter BrisouandPrevot 1954, 727.. In Krieg N. R. (ed) Bergey’s Manual of systematic bacteriology vol 1 Williams and Wilkins; Baltimore: pp 303–307
    [Google Scholar]
  16. Loeb M. R., Smith D. H. 1980; Outer membrane protein composition in disease isolates of Haemophilus influenzae: pathogenic and epidemiological implications.. Infection and Immunity 30:709–717
    [Google Scholar]
  17. Lugtenberg B., Meijers J., Peters R., der Hoek P., van Alphen L. 1975; Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K12 into four bands. FEBS Letters 58:254–258
    [Google Scholar]
  18. Lugtenberg B., Peters R., Bemheimer H., Berendsen W. 1976; Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Molecular and General Genetics 147:251–262
    [Google Scholar]
  19. Lugtenberg B., van Boxtel R., de Jong M. 1984; Atrophic rhinitis in swine: correlation of Pasteurella multocida pathogenicity with membrane protein and lipopolysaccharide patterns. Infection and Immunity 46:48–54
    [Google Scholar]
  20. Marcus B. B., Samuels S. B., Pittman B., Cherry W. B. 1969; A serologic study of Herellea vaginicola and its identification by immunofluorescent staining. American Journal of Clinical Pathology 52:309–319
    [Google Scholar]
  21. Mocca L. F., Frasch C. E. 1982; Sodium dodecyl sulfate-polyacrylamide gel typing system for characterization of Neisseria meningitidis isolates. Journal of Clinical Microbiol ogy 16:240–244
    [Google Scholar]
  22. Monod J., Wollman E. 1947; l’lnhibition de la croissance et de l’adaptation enzymatique chez les bacteries infectees par le bacteriophage. Annales de Vlnstitut Pasteur 73:937–956
    [Google Scholar]
  23. Overbeeke N., Lugtenberg B. 1980; Major outer membrane proteins of Escherichia coli strains of human origin. Journal of General Microbiology 121:373–380
    [Google Scholar]
  24. Ramphal R., Kluge R. M. 1979; Acinetobacter calcoaceticus variety anitratus: an increasing nosocomial problem. American Journal of the Medical Sciences 211:57–66
    [Google Scholar]
  25. Rosenthal S. L. 1978; Clinical role of Acinetobacter and Moraxella. In Gilardi G. L. (ed) Glucose nonfermenting Gram-negative bacteria in clinical microbiology CRC Press; West Palm Beach, FL: pp 109–110
    [Google Scholar]
  26. Sherertz R. J., Sullivan M. L. 1985; An outbreak of infections with Acinetobacter calcoaceticus in burn patients: contamination of patients’ mattresses. Journal of Infectious Diseases 151:252–258
    [Google Scholar]
  27. Sierra G. 1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15–22
    [Google Scholar]
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