1887

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Volume 23, Issue 3

Characterisation of anaerobic curved rods ( spp.) isolated from the urogenital tract Free

Abstract

Summary

Thirty-two strains of anaerobic curved rods isolated from vaginal secretions and one isolated from seminal fluid were examined. Growth of all strains on solid media was superior to growth in liquid media, and at 37°C they grew both anaerobically and in O 5% in N; they also grew anaerobically at 33°C but not at 42°C. No growth factors were identified, but strains grew more profusely atH values above 5–0. The strains were screened in 80 biochemical tests, and for their susceptibility to 30 different antimicrobial agents. Most of the tests did not differentiate between the strains, but they were divided into four groups on the basis of cell morphology, metronidazole susceptibility, β-galactosidase activity and arginine and hippurate hydrolysis. Group 1 consisted of 19 strains conforming to the species ; group 2 consisted of five strains conforming to the species ; group 3 consisted of five strains that resembled morphologically, and group 4 consisted of four strains that resembled morphologically, but the strains in the latter two groups reacted differently in at least one of the three major differential biochemical tests. Of three strains of and three of chosen at random, one of had a SDS-PAGE and fast-protein liquid chromatography protein profile indistinguishable from that of We conclude that further efforts are required to clarify the taxonomic status of the genus

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1987
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1987-05-01
2024-04-20
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References

  1. Baron E. J., Wexler H. M., Finegold S. M. 1984; Biochemical and polyacrylamide gel electrophoretic analyses of vaginosis-associated anaerobic curved rods. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiskell; Stockholm: pp 65–69
     [Google Scholar]
  2. Blackwell A. L., Fox A. R., Phillips I., Barlow D. 1983; Anaerobic vaginosis (non-specific vaginitis): clinical, microbiological and therapeutic findings. Lancet 2:1379–1382
     [Google Scholar]
  3. Borriello S. P., Reed P. J., Barclay F. E. 1985; Identification of bacteria by fast protein liquid chromatography. Journal of Medical Microbiology 19:ix
     [Google Scholar]
  4. Borriello S. P., Reed P. J., Barclay F. E. 1986; The use of fast protein liquid chromatography for the identification of anaerobic bacteria. In Borriello S. P., Hardie J. M. (eds) Recent Advances in Anaerobic Microbiology Martinus Nijhoff Publishers BV; The Hague: pp 292–294
     [Google Scholar]
  5. Christiansen G., Hansen E., Holst E., Christiansen C., Mardh P.-A. 1984; Genetic relationships of short and long anaerobic curved rods isolated from the vagina. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wilksell; Stockholm: pp 75–78
     [Google Scholar]
  6. Cowan S. T. 1974; Cowan and Steel’s Manual for the Identification of Medical Bacteria. 2nd edn Cambridge University Press; Cambridge:
     [Google Scholar]
  7. Durieux R., Dublanchet A. 1980; Les “vibrions” anaerobies des leucorrhees I. Technique d’isolement et sensibilite aux antibiotiques. Medecine et Maladies Infectieuses 10:109–115
     [Google Scholar]
  8. Fox A., Phillips I. 1984; Two curved rods in non-specific vaginitis. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiskell; Stockholm: pp 93–96
     [Google Scholar]
  9. Hjelm E., Forsum U., Hallen A., Pahlson C., Wallin J. 1984; Primary isolation of curved rods from women with vaginal discharge. In Mardh P.-A., Robinson D. (eds) Bacterial vaginosis Almqvist and Wiskell; Stockholm: pp 113–116
     [Google Scholar]
  10. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe laboratory manual. 4th edn Virginia Polytechnic Institute and State University; Blacksburg, VA, USA:
     [Google Scholar]
  11. Holst E., Skarin A., Mardh P.-A. 1982; Characteristics of anaerobic comma-shaped bacteria recovered from the female genital tract. European Journal of Clinical Microbiology 1:310–316
     [Google Scholar]
  12. Jones B. M., Geary I., Alawattegama A. B., Kinghorn G. R., Duerden B. I. 1985; In-vitro and in-vivo activity of metroni dazole against Gardnerella vaginalis, Bacteroides spp. and Mobiluncus spp. in bacterial vaginosis. Journal of Antimicro bial Chemotherapy 16:189–197
     [Google Scholar]
  13. Moi H., Danielsson D. 1984; Studies on rabbit hyperimmune, patient and blood donor serum with regard to bactericidal activity and serum antibodies against anaerobic curved rods from patients with bacterial vaginosis. In Mardh P. A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 87–91
     [Google Scholar]
  14. Nord C. E. 1984; The diagnostic criteria for anaerobic curved rods. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: p 262
     [Google Scholar]
  15. Owen R. 1984; Taxonomy of anaerobic curved rods. In Mardh P.-A., Robinson D. (eds) Bacterial vaginosis Almqvist and Wiskell; Stockholm: pp 264–265
     [Google Scholar]
  16. Pahlson C., Bergqvist F., Forsum U. 1984; Numerical taxonomy of motile anaerobic curved rods isolated from vaginal discharge. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 251–256
     [Google Scholar]
  17. Phillips I., Taylor E. 1982; Anaerobic curved rods in vaginitis. Lancet 1:221
     [Google Scholar]
  18. Skarin A., Mardh P.-A. 1982; Comma-shaped bacteria associated with vaginitis. Lancet 1:342–343
     [Google Scholar]
  19. Skarin A., Weibull C., Mardh P.-A. 1984; Light and electron microscopic studies of anaerobic curved bacteria isolated from the vagina. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 59–64
     [Google Scholar]
  20. Spiegel C. A., Amsel R., Eschenbach D., Schoenknecht F., Holmes K. K. 1980; Anaerobic bacteria in non-specific vaginitis. NewEnglandJournalofMedicine 303:601–607
     [Google Scholar]
  21. Spiegel Eschenbach D. A., Amsel R., Holmes K. K. 1983; Curved anaerobic bacteria in bacterial (nonspecific) vaginosis and their response to antimicrobial therapy. JournalofInfectiousDiseases 148:817–822
     [Google Scholar]
  22. Spiegel C. A., Roberts M. 1984; Mobiluncus gen. nov., Mobiluncuscurtisii subspecies curtisii sp. nov., Mobiluncuscurtisii subspecies holmesii subs nov. and Mobiluncusmulieris sp. nov., curved rods from the human vagina. International Journal of Systematic Bacteriology 34:177–184
     [Google Scholar]
  23. Sprott M. S., Pattman R. S., Ingham H. R., Short G. R., Narang H. K., Selkon J. B. 1982; Anaerobic curved rods in vaginitis. Lancet 1:54
     [Google Scholar]
  24. Sprott M. S. 1983; Characteristics of motile curved rods in vaginal secretions. Journal of Medical Microbiology 16:175–182
     [Google Scholar]
  25. Sprott M. S., Ingham H. R., Pattman R. S., Clarkson L. M., Codd A. A., Narang H. K. 1984; Motile curved bacilli: isolation and investigation. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 107–111
     [Google Scholar]
  26. Sutter V. L., Citron D. M., Finegold S. M. 1980; Wadsworth anaeorobic bacteriology manual. 3rd edn C. V. Mosby Company, St Louis, MO; U.S.A:
     [Google Scholar]
  27. Taylor A. J., Owen R. J. 1984; Morphological and chemical characteristics of anaerobic curved rod-shaped bacteria from the female genital tract. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 97–106
     [Google Scholar]
  28. Thomason J. L., Schreckenberger P. C., LeBeau L. J., Wilcoski L. M., Spellacy W. N. 1984; A selective and differential agar for anaerobic comma-shaped bacteria recovered from patients having motile rods and non-specific vaginosis. In Mardh P.-A., Taylor-Robinson D. (eds) Bacterial vaginosis Almqvist and Wiksell; Stockholm: pp 125–128
     [Google Scholar]
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Characterisation of anaerobic curved rods (Mobiluncus spp.) isolated from the urogenital tract
J. Med. Microbiol. 23, 279 (1987); https://doi.org/10.1099/00222615-23-3-279
/content/journal/jmm/10.1099/00222615-23-3-279
/content/journal/jmm/10.1099/00222615-23-3-279
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