Accumulation of a precursor of subunit S1 of pertussis toxin in cell envelopes of in response to the membrane perturbant phenethyl alcohol Free

Abstract

Summary

Exponential cultures of strain 18334 were treated with the membrane-perturbing agent phenethyl alcohol which, at a concentration of 0.075% v/v, blocked the synthesis of mature subunit S1 of pertussis toxin as revealed by Western blotting. It also caused the accumulation of a precursor, pS1, with an estimated mol. wt of 32 × 10, that was located in the cytoplasmic membrane. These findings suggested that subunit S1 of pertussis toxin was exported in a signal peptidedependent manner.

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1987-05-01
2024-03-29
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References

  1. Altenbern R. A. 1977; Extreme sensitivity of staphylococcal enterotoxin B and C production to inhibition by cerulenin. Antimicrobial Agents and Chemotheraphy 11:906–908
    [Google Scholar]
  2. Berkeley R. C. W., Pepper E. A., Caulfield M. P., Melling J. 1978; The inhibition of Staphylococcusaureus enterotoxin A production by cerulenin and quinacrine; presumptive evidence for a lipid intermediate protease release mechanism. FEMS Microbiology Letters 4:103–105
    [Google Scholar]
  3. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. I. Journal of Cell Biology 67:835–851
    [Google Scholar]
  4. Halegoua S., Inouye M. 1979; Translocation and assembly of outer membrane proteins of Escherichia coli. Selective accumula tion of percursors and novel assembly intermediates caused by phenethyl alcohol. Journal of Molecular Biology 130:39–61
    [Google Scholar]
  5. Hall M. N., Silhavy T. J. 1981; Genetic analysis of the major outer membrane proteins of Escherichia coli. Annual Review of Genetics 15:91–142
    [Google Scholar]
  6. Hirst T. R., Hardy S. J. S., Randall L. L. 1983; Assembly in vivo of enterotoxin from Escherichia coli: formation of the B subunit oligomer. Journal of Bacteriology 153:21–26
    [Google Scholar]
  7. Hirst T. R., Randall L. L., Hardy S. J. S. 1984; Cellular location of heat-labile enterotoxin in Escherichia coli. Journal of Bacteriology 157:637–642
    [Google Scholar]
  8. Hofstra H., Dorner F., Kingma J., Witholt B. 1984; Escherichia coli LT enterotoxin and its relationship to the cell envelope. In Alouf J. E. (eds) Bacterial protein toxins Academic Press; London: pp 89–97
    [Google Scholar]
  9. Imagawa T., Sonoda S., Kanoh M., Utsumi S. 1979; Polymorpho nuclear leukocyte-inhibitory factor of Bordetella pertussis. II. Localization in the outer membrane. Biken Journal 22:1–10
    [Google Scholar]
  10. Imaizumi A., Suzuki Y., Ono S., Sato H., Sato Y. 1983; Effect of heptakis (2, 6-O-dimethyl) 1-cyclodextrin on the production of pertussis toxin by Bordetella pertussis. Infection and Immunity 41:1138–1143
    [Google Scholar]
  11. Inuoye M., Halegoua S. 1980; Secretion and membrane localization of proteins in Escherichia coli. CRCCritical Reviews in Biochemistry 1:339–371
    [Google Scholar]
  12. Lory S., Tai P. C., Davis B. D. 1983; Mechanism of protein excretion by gram-negative bacteria: Pseudomonasaeruginosa exo toxin A. Journal of Bacteriology 156:695–702
    [Google Scholar]
  13. Morse J. H., Morse S. I. 1970; Studies on the ultrastructure of Bordetellapertussis. I. Morphology, origin and biological activity of structures present in the extracellular fluid of liquid cultures of Bordetella pertussis. Journal ofExperimental Medicine 131:1342–1357
    [Google Scholar]
  14. Nicosia A. 1986; Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication. Proceedings of the National Acadeny of Sciences of the USA 83:4631–4635
    [Google Scholar]
  15. Osborn M. J., Gander J. E., Parisi E., Carson J. 1972; Mechanism of assembly of the outer membrane of Salmonellatyphimurium. Isolation and characterization of cytoplasmic and outer membrane. Journal of Biological Chemistry 1:A13962–3972
    [Google Scholar]
  16. Pages J. M., Lazdunski C. 1981; Action of phenethyl alcohol on the processing of precursor forms of periplasmic proteins in Escherichia coli. FEMS Microbiology Letters 12:65–69
    [Google Scholar]
  17. Palva E. T., Hirst T. R., Hardy S. J. S., Holmgren J., Randall L. L. 1981; Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli. Journal of Bacteriology 146:325–330
    [Google Scholar]
  18. Perera V. Y., Penn C. W., Smith H. 1982; Purification of pili and outer membrane vesicles of Neisseria gonorrhoeae by wheat germ agglutinin affinity chromatography. Journal of General Microbiology 128:1613–1622
    [Google Scholar]
  19. Perera V. Y., Rogers H., Freer J. H. 1985; One-dimensional peptide mapping of the subunits of pertussis toxin. Journal of General Microbiology 131:1897–1901
    [Google Scholar]
  20. Perera V. Y., Wardlaw A. C., Freer J. H. 1986a In Falmagne P. (ed) Bacterial protein toxins. Proceedings of the 2nd European Conference on Bacterial Protein Toxins, Wepion Fischer Verlag; Berlin (in press):
    [Google Scholar]
  21. Perera V. Y., Wardlaw A. C., Freer J. H. 1986b; Antigenic heterogeneity in subunit SI of pertussis toxin. JournalofGeneralMicrobiology 132:553–556
    [Google Scholar]
  22. Peterson G. L. 1977; A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  23. Pugsley A. P., Schwartz M. 1985; Export and secretion of proteins by bacteria. FEMS Microbiology Reviews 32:3–38
    [Google Scholar]
  24. Randall L. L., Hardy S. J. S., Josefsson L. G. 1978; Precursors of three exported proteins in Escherichia coli. ProceedingsoftheNationalAcademyofSciencesoftheUSA 75:1209–1212
    [Google Scholar]
  25. Sekura R. D., Fish F., Manclark C. R., Meade B., Zhang Y.-L. 1983; Pertussis toxin. Affinity purification of a new ADP-ribosyltransferase. Journal of Biological Chemistry 258:14647–14651
    [Google Scholar]
  26. Silhavy T. J., Benson S. A., Emr S. D. 1983; Mechanisms of protein localization. Microbiological Reviews 47:313–344
    [Google Scholar]
  27. Silver S., Wendt L. 1967; Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier. Journal of Bacteriology 93:560–566
    [Google Scholar]
  28. Tamura 1982; Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model. Biochem istry 21:5516–5522
    [Google Scholar]
  29. Wardlaw A. C., Parton R. 1982; Bordetella pertussis toxins. Pharmacology and Therapeutics 19:1–53
    [Google Scholar]
  30. Work E., Knox K. W., Vesk M. 1966; The chemistry and electron microscopy of an extracellular lipopolysaccharide from Escherichia coli. Annals of New York Academy of Science 133:438–449
    [Google Scholar]
  31. Yajima M. 1978; Islets-activatingprotein (IAP) in Bordetellapertussis that potentiates insulin secretory responses of rats. Journal of Biochemistry, Tokyo 83:295–303
    [Google Scholar]
  32. Yoshikawa M., Matsuda F., Naka M., Murofushi E., Tsunematsu Y. 1974; Pleiotropic alteration of activities of several toxins and enzymes in mutants of Staphylococcus aureus. Journal of Bacteriology 119:117–122
    [Google Scholar]
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