RT Journal Article SR Electronic(1) A1 Ollar, R. A. A1 Stewart-Tull, D. E. S.YR 1987 T1 Studies on the Vibrio cholerae mucinase complex. II. Specific neuraminidase activity measured histochemically in a goblet cell assay JF Journal of Medical Microbiology, VO 23 IS 3 SP 227 OP 232 DO https://doi.org/10.1099/00222615-23-3-227 PB Microbiology Society, SN 1473-5644, AB Summary The activity of neuraminidase prepared from the mucinase complex of Vibrio cholerae was measured by a new, semi-quantitative goblet-cell assay. The counts of normal, alcianophilic, sialomucin-containing goblet cells (purple-stained) and neutral mucosubstance-containing goblet cells (magenta-stained) in serial sections of ileum were compared before and after neuraminidase treatment. The procedure provides a more natural assessment of the action of V. cholerae neuraminidase on the viscous intestinal mucus., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-23-3-227