1887

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Volume 23, Issue 2

cytotonic enterotoxin cross reactive with cholera toxin Free

Abstract

Summary

Isolation by affinity chromatography from crude culture filtrate of of protein that cross reacted with cholera toxin (CT) revealed a toxin that produced fluid accumulation in rat ileal loops and in infant mice and caused rounding of Y1 adrenal cells. All these activities were neutralised by antiserum to CT. There was no haemolytic or cytotoxic activity associated with this CT-cross reactive cytotonic enterotoxin. CT-cross reactive material detected in enzyme linked immunosorbent assay (ELISA) was produced by 25% of isolates from faeces of children with or without diarrhoea—26% of , 20.0% of and 24% of tested gave positive ELISA results. Most strains that produced this cytotonic enterotoxin but no cytotoxic enterotoxin were isolated from children without diarrhoea. Toxin preparations from spp. that completely inhibited adenosine-5′-diphosphate-induced platelet aggregation, an effect related to elevation of intracellular cAMP, were, with one exception, cross reactive with CT in ELISA.

  • Received:
  • Accepted:
  • Published Online:
© J. MED. MICROBIOL. 1987
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1987-03-01
2024-04-20
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References

  1. Asao T., Kinoshita Y., Kozaki S., Uemura T., Sakaguchi G. 1984; Purification and some properties of Aeromonas hydrophila hemolysin. Infection and Immunity 46:122–127
     [Google Scholar]
  2. Baselski V., Briggs R., Parker C. 1977; Intestinal fluid accumulation induced by oral challenge with Vibrio cholerae or cholera toxin in infant mice. Infection and Immunity 15:704–712
     [Google Scholar]
  3. Born G. V. R. 1962; Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 194:927–929
     [Google Scholar]
  4. Boulanger Y., Lallier R., Cousineau G. 1977; Isolation of enterotoxigenic Aeromonas from fish. Canadian Journal of Microbiology 23:1161–1164
     [Google Scholar]
  5. Burke V. 1984a; Hemagglutination patterns of Aeromonas spp. in relation to biotype and source. Journal of Clinical Microbiology 19:39–43
     [Google Scholar]
  6. Burke V., Robinson J., Berry R. J., Gracey M. 1981; Detection of enterotoxins of Aeromonas hydrophila by a suckling mouse test. Journal of Medical Microbiology 14:401–408
     [Google Scholar]
  7. Burke V. 1984b; Biotyping and virulence factors in clinical and environmental isolates of Aeromonas spp. Applied and Environmental Microbiology 47:1146–1149
     [Google Scholar]
  8. Campbell J. D., Houston C. W. 1985; Effect of cultural conditions on the presence of a cholera-toxin cross reactive factor in culture filtrates of Aeromonas hydrophila. Current Microbiology 12:101–106
     [Google Scholar]
  9. Chakraborty T., Montenegro M. A., Sanyal S. C., Helmuth R., Bulling E., Timmis K. N. 1984; Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin. Infection and Immunity 46:435–441
     [Google Scholar]
  10. Clements J. D., Finkelstein R. A. 1978; Immunological crossreactivity between a heat-labile enterotoxin(s) of Escherichia coli and subunits of Vibrio cholerae enterotoxin. Infection and Immunity 21:1036–1039
     [Google Scholar]
  11. Dafni Z., Robbins J. B. 1976; Purification of heat-labile enterotoxin from Escherichia coli 078: HI 1 by affinity chromatography with antiserum to Vibrio cholerae toxin. Journal of Infectious Disease 133: Supplement S138–S141
     [Google Scholar]
  12. Dafni Z., Sack R. B., Craig J. P. 1978; Purification of heat-labile enterotoxin from four Escherichia coli strains by affinity immunoadsorbent: evidence for similar subunit structure. Infection and Immunity 22:852–860
     [Google Scholar]
  13. Dobrescu L. 1978; Enterotoxigenic Aeromonas hydrophila from a case of piglet diarrhoea. Zentralblatt fur Veterinarmedizin B 25:713–718
     [Google Scholar]
  14. Donta S. T., Moon H. W., Whipp S. C. 1974; Detection of heat- labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science 183:334–336
     [Google Scholar]
  15. Dubey R. S., Bhattacharya A. K., Sanyal S. C. 1981; Elevation of adenosine 3′5′ cyclic monophosphate level by Aeromonas hydrophila enterotoxin. Indian Journal of Medical Research 74:668–674
     [Google Scholar]
  16. Fumarola D., Miragliotta G. 1981; Platelet aggregation test and enterotoxins from Yersinia enterocolitica. IRCS Medical Science 9:1033
     [Google Scholar]
  17. Fumarola D., Miragliotta G. 1983; Platelet aggregation test (PAT): an assay system to evaluate enterotoxins. Abstracts of the 83rd Annual Meeting of the American Society of Microbiology Abstract no. 1399 p 40
     [Google Scholar]
  18. Fumarola D., Miragliotta G. 1984; Aeromonas toxins. Pediatric Infectious Disease 3:490–491
     [Google Scholar]
  19. Gracey M., Burke V., Robinson J. 1982; Aeromonas-associated gastroenteritis. Lancet 2:1304–1306
     [Google Scholar]
  20. James C., Dibley M., Burke V., Robinson J., Gracey M. 1982; Immunological cross-reactivity of enterotoxins of Aeromonas hydrophila and cholera toxin. Clinical and Experimental Immunology 47:34–42
     [Google Scholar]
  21. Jiwa S. F. H. 1983; Enterotoxigenicity, hemagglutination and cell surface hydrophobicity in Aeromonas hydrophila, Aeromonas sobria and Aeromonas salmonicida. Veterinary Microbiology 81:17–34
     [Google Scholar]
  22. Klipstein F. A., Engert R. F. 1977; Immunological interrelationships between cholera toxin and the heat-labile and heat- stable enterotoxins of coliform bacteria. Infection and Immunity 18:110–117
     [Google Scholar]
  23. Lambin P. 1978; Reliability of molecular weight determination of proteins by polyacrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate. Analytical Biochemistry 85:114–125
     [Google Scholar]
  24. Ljungh A., Popoff M., Wadstrom T. 1977; Aeromonas hydrophila in acute diarrheal disease: detection of enterotoxin and biotyping of strains. Journal of Clinical Microbiology 6:96–100
     [Google Scholar]
  25. Ljungh A., Wretlind B., Mollby R. 1981; Separation and characterization of enterotoxin and two haemolysins from Aeromonas hydrophila. Acta Pathologica et Microbiologica Scandinavica, Section B 89:387–397
     [Google Scholar]
  26. Pierce N. F. 1977; Protection against challenge with Escherichia coli heat-labile enterotoxin by immunization of rats with cholera toxin/toxoid. Infection and Immunity 18:338–341
     [Google Scholar]
  27. Popoff M. 1984; Aeromonas Kluyver and Van Niel 1936. In Krieg N. R., Holt J. G. (eds) Bergey’s Manual of systemic bacteriology vol 1 9th edn Williams and Wilkins Co; Baltimore:545–548
     [Google Scholar]
  28. Read S. M., Northcote D. H. 1981; Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Analytical Biochemistry 116:53–64
     [Google Scholar]
  29. Ruiz-Palacios G. M., Torres J., Torres N. I., Escamilla E., Palacios B. R., Tamayo J. 1983; Cholera-like enterotoxin produced by Campylobacter jejuni: Characterisation and clinical significance. Lancet 2:250–253
     [Google Scholar]
  30. Salzman E. W. 1972; Cyclic AMP and platelet function. New England Journal of Medicine 286:358–363
     [Google Scholar]
  31. Sandefur P. D., Peterson J. W. 1977; Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin. Infection and Immunity 15:988–992
     [Google Scholar]
  32. Satterwhite T. K., Evans D. G. S, Dupont H. L., Evans D. J. 1978; Role of Escherichia coli colonisation factor antigen in acute diarrhoea. Lancet 2:181–184
     [Google Scholar]
  33. Schubert R. H. W. 1967; Die Pathogenitat der Aeromonaden fur Mensch und Tier. Archiv fur Hygiene und Bakteriologie 150:709–716
     [Google Scholar]
  34. Shimada T., Sakazaki R., Horigome K., Uesaka Y., Niwano K. 1984; Production of cholera-like enterotoxin by Aeromonas hydrophila. Japanese Journal of Medical Science and Biology 37:141–144
     [Google Scholar]
  35. Wadstrom T., Ljungh A., Wretlind B. 1976; Enterotoxin, haemo- lysin and cytotoxic protein in Aeromonas hydrophila from human infections. Acta Pathologica et Microbiologica Scandinavica 84:112–114
     [Google Scholar]
  36. Watson I. M., Robinson J. O., Burke V., Gracey M. 1985; Invasiveness of Aeromonas spp. in relation to biotype, virulence factors and clinical features. Journal of Clinical Microbiology 22:48–51
     [Google Scholar]
  37. Yolken R. H., Greenberg H. B., Merson M. H., Sack R. B., Kapikian A. Z. 1977; Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. Journal of Clinical Microbiology 6:439–444
     [Google Scholar]
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Aeromonas cytotonic enterotoxin cross reactive with cholera toxin
J. Med. Microbiol. 23, 179 (1987); https://doi.org/10.1099/00222615-23-2-179
/content/journal/jmm/10.1099/00222615-23-2-179
/content/journal/jmm/10.1099/00222615-23-2-179
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