1887

Abstract

Summary

Cytotoxic enterotoxin of was purified by affinity chromatography with monoclonal antibodies. The purified enterotoxin gave a single protein band in polyacrylamide gradient gel electrophoresis and its mol. wt estimated by this technique was 63000; it had a pI of 6.2. The purified enterotoxin caused fluid accumulation in rat ileal loops and in infant mice, was cytotoxic to cultured cells, was haemolytic to human erythrocytes, and was lethal to mice after intravenous injection. The relative concentrations of enterotoxic, cytotoxic and haemolytic activities were approximately the same in a culture filtrate and in purified, electrophoretically homogeneous enterotoxin. The three activities were also inactivated to the same extent after incubation for 10 min at 56°C. There was no immunological cross-reactivity with cholera toxin (CT) nor did antiserum to CT neutralise the biological effects of the toxin.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-23-2-171
1987-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/23/2/medmicro-23-2-171.html?itemId=/content/journal/jmm/10.1099/00222615-23-2-171&mimeType=html&fmt=ahah

References

  1. Asao T., Kinoshita Y., Kozaki S., Uemura T., Sakaguchi G. 1984; Purification and some properties of Aeromonas hydrophila hemolysin. Infection and Immunity 46:122–127
    [Google Scholar]
  2. Burke V., Robinson J., Atkinson H. M., Dibley M., Berry R. J., Gracey M. 1981; Exotoxins of Aeromonas hydrophila. Australian Journal of Experimental Biology and Medical Science 59:753–761
    [Google Scholar]
  3. Burke V., Robinson J., Berry R. J., Gracey M. 1981; Detection of enterotoxins of Aeromonas hydrophila with suckling-mouse test. Journal of Medical Microbiology 14:401–408
    [Google Scholar]
  4. Campbell J. D., Houston C. W. 1985; Effect of cultural conditions on the presence of a cholera-toxin cross-reactive factor in culture filtrates of Aeromonas hydrophila. Current Microbiology 12:101–106
    [Google Scholar]
  5. Chakraborty T., Sanyal S. C., Helmuth R., Bulling E., Timmis K. N. 1984; Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin. Infection and Immunity 46:435–441
    [Google Scholar]
  6. Cumberbatch N., Gurwith M. J., Langston C., Sack R. B., Brunton J. L. 1979; Cytotoxic enterotoxin produced by Aeromonas hydrophila: relationship of toxigenic isolates to diarrheal disease. Infection and Immunity 23:829–837
    [Google Scholar]
  7. Dobrescu L. 1978; Enterotoxigenic Aeromonas hydrophila from a case of piglet diarrhoea. Zentralblatt fur Veterinarmedizin B 25:713–718
    [Google Scholar]
  8. Galfre G., Milstein C. 1981; Preparation of monoclonal antibodies:strategies and procedures. Methods in Enzymology 73:3–46
    [Google Scholar]
  9. Gemmell C. G. 1984; Comparative study of the nature and biological activities of bacterial enterotoxins. Journal of Medical Microbiology 17:217–235
    [Google Scholar]
  10. Hostacka A., Ciznar I., Korych B., Karolcek J. 1982; Toxic factors of Aeromonas hydrophila and Plesiomonas shigelloides. Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. I Abteilung Originale A 252:525–534
    [Google Scholar]
  11. Inoue T., Okamoto K., Moriyama T., Takahashi T., Shimizu K., Miyama A. 1983; Effect of Yersinia enterocolitica ST on cyclic guanosine 3′5′ monophosphate levels in mouse intestines and cultured cells. Microbiology and Immunology 27:159–166
    [Google Scholar]
  12. James C., Dibley M., Burke V., Robinson J., Gracey M. 1982; Immunological cross-reactivity of enterotoxins of Aeromonas hydrophila and cholera toxin. Clinical and Experimental Immunology 47:34–42
    [Google Scholar]
  13. Janossay G., Tarjan V. 1980; Enterotoxigenicity of Aeromonas strains in suckling mice. Acta Microbiologica Academiae Scientiarum Hungaricae 27:63–69
    [Google Scholar]
  14. Jiwa S. F. H. 1983; Enterotoxigenicity, hemagglutination and cell surface hydrophobicity in Aeromonas hydrophila, Aeromonas sobria and Aeromonas salmonicida. Veterinary Microbiology 8:17–34
    [Google Scholar]
  15. Johnson W. M., Lior H. 1981; Cytoxicity and suckling mouse reactivity of Aeromonas hydrophila isolated from human sources. Canadian Journal of Microbiology 27:1019–1027
    [Google Scholar]
  16. Klipstein F. A., Engert R. F., Houghten R. A. 1983; Immunological properties of purified Klebsiella pneumoniae heat-stable enterotoxin. Infection and Immunity 42:838–841
    [Google Scholar]
  17. Ljungh A., Wadstrom T. 1982; Aeromonas toxins. Pharmacology and Therapeutics 15:339–354
    [Google Scholar]
  18. Ljungh A., Wretlind B., Mollby R. 1981; Separation and characterization of enterotoxin and two haemolysins from Aeromonas hydrophila. Acta Pathologica et Microbiologica Scandinavica. Section B 89:387–397
    [Google Scholar]
  19. Margolis J., Kenrick K. G. 1968; Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient. Analytical Biochemistry 25:347–362
    [Google Scholar]
  20. Okamoto K., Inoue T., Moriyama T., Miyama A. 1983; Heat-stable enterotoxin produced by Yersinia enterocolitica. Toxicon 3:325–328
    [Google Scholar]
  21. Olivier G., Lallier R., Lariviere S. 1981; A toxigenic profile of Aeromonas hydrophila and Aeromonas sobria isolated from fish. Canadian Journal of Microbiology 21:330–333
    [Google Scholar]
  22. Pierce N. F. 1977; Protection against challenge with Escherichia coli heat-labile enterotoxin by immunization of rats with cholera toxin/toxoid. Infection and Immunity 18:338–341
    [Google Scholar]
  23. Popoff M. 1984; AeromonasKluyver and Van Niel 1936. In Krieg N. R., Holt J. G. (eds) Bergey’s Manual of systematic bacteriology vol 1 9th edn Williams and Wilkins Co; Baltimore: pp 545–548
    [Google Scholar]
  24. Read S. M., Northcote D. H. 1981; Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Analytical Biochemistry 116:53–64
    [Google Scholar]
  25. Shimada T., Sakazaki R., Horigome K., Uesaka Y., Niwano K. 1984; Production of cholera-like enterotoxin by Aeromonas hydrophila. Japanese Journal of Medical Science and Biology 37:141–144
    [Google Scholar]
  26. Thompson N. E., Ketterhagen M. J., Bergdoll M. S., Schantz E. J. 1984; Isolation and some properties of an enterotoxin produced by Bacillus cereus. Infection and Immunity 43:887–894
    [Google Scholar]
  27. Turnbull P. C. B. 1984; Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. Journal of Clinical Microbiology 19:175–180
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-23-2-171
Loading
/content/journal/jmm/10.1099/00222615-23-2-171
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error