1887

Abstract

Summary

Resistance to gentamicin, tobramycin and kanamycin (GmTmKm) in strains of isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn. A 2.5 kb dIII fragment which maps symmetrically within Tn, and encompasses the aminoglycosideresistance coding region, has been shown to hybridise with fragments of identical size in dIII digests of three different GmTmKm plasmids, two of which were self-transmissible, from strains of isolated in the USA. Examination by electronmicroscopy of self-annealed molecules of the North American GmTmKm plasmids revealed the presence of stem and loop structures similar to those produced by Tn, but with shorter inverted repeats. These results suggest that GmTmKm in strains of isolated in the USA is, or once was, transposable, and that transposable elements analogous to Tn may be found in isolates of GmTmKm worldwide.

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1987-03-01
2024-12-05
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References

  1. Archer G. L., Johnston J. L. 1983; Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides. Antimicrobial Agents and Chemotherapy 24:70–77
    [Google Scholar]
  2. Archer G. L., Dietrick D. R., Johnston J. L. 1985; Molecular epidemiology of transmissible gentamicin resistance among coagulase-negative staphylococci in a cardiac surgery unit. Journal of Infectious Diseases 151:243–251
    [Google Scholar]
  3. Asch D. K., Goering R. V., Ruff E. A. 1984; Isolation and preliminary characterization of a plasmid mutant derepressed for conjugal transfer in Staphylococcus aureus. Plasmid 12:197–202
    [Google Scholar]
  4. Cohen M. L., Wong E. S., Falkow S. 1982; Common R-plasmids in Staphylococcus aureus and Staphylococcus epidermidis during a nosocomial Staphylococcus aureus outbreak. Antimicrobial Agents and Chemotherapy 21:210–215
    [Google Scholar]
  5. Crossley K., Loesch D., Landesman B., Mead K., Chern M., Strate R. 1979; An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. I. Clinical studies. Journal of Infectious Diseases 139:273–279
    [Google Scholar]
  6. Daniels D. L., Schroeder J. L., Szybalski W., Sanger F., Blattner F. R. 1983; A molecular map of coliphage lambda. In Hendrix R. W. (eds) Lambda II Cold Spring Harbor Laboratory, Cold Spring Harbor; NY: p 469
    [Google Scholar]
  7. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  8. Dowd G., Cafferkey M., Dougan G. 1983; Gentamicin and methicillin resistant Staphylococcus aureus in Dublin hospitals: molecular studies. Journal of Medical Microbiology 16:129–138
    [Google Scholar]
  9. ElSolh N., Fouace J. M., Pillet J., Chabbert Y. A. 1981; Plasmid DNA content of multiresistant Staphylococcus aureus strains. Annales de Microbiologie 132B:131–156
    [Google Scholar]
  10. Gillespie M. T., Skurray R. A. 1986; Plasmids in multiresistant Staphylococcus aureus. Microbiological Sciences 3:53–58
    [Google Scholar]
  11. Gillespie M. T., May J. W., Skurray R. A. 1984; Antibiotic susceptibilities and plasmid profiles of nosocomial methi- cillin-resistant Staphylococcus aureus: A retrospective study. Journal of Medical Microbiology 17:295–310
    [Google Scholar]
  12. Goering R. V., Ruff E. A. 1983; Comparative analysis of conjugative plasmids mediating gentamicin resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 24:450–452
    [Google Scholar]
  13. Gray G. S., Davies J. 1983; Aminocyclitol resistance in Staphylococcus aureus: Presence of plasmids and amino- cyclitol-modifying enzymes. Plasmid 9:147–158
    [Google Scholar]
  14. Hone R. 1981; Bacteraemia in Dublin due to gentamicin resistant Staphylococcus aureus. Journal of Hospital Infection 2:119–126
    [Google Scholar]
  15. Horinouchi S., Weisblum B. 1982; Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. Journal of Bacteriology 150:815–825
    [Google Scholar]
  16. Jaffe H. W., Sweeney H. M., Weinstein R. A., Kabins S. A., Nathan C., Cohen S. 1982; Structural and phenotypic varieties of gentamicin resistance plasmids in hospital strains of Staphylococcus aureus and coagulase-negative staphylococci. Antimicrobial Agents and Chemotherapy 21:773–779
    [Google Scholar]
  17. Kayser F. H., Homberger F., Devaud M. 1981; Aminocyclitol- modifying enzymes specified by chromosomal genes in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 19:766–772
    [Google Scholar]
  18. LeGoffic F., Martel A., Moreau N., Capmau M. L., Soussy C. J., Duval J. 1977; 2′′-O-Phosphorylation of gentamicin components by a Staphylococcus aureus strain carrying a plasmid. Antimicrobial Agents and Chemotherapy 12:26–30
    [Google Scholar]
  19. Lyon B. R., May J. W., Skurray R. A. 1983; Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 23:817–826
    [Google Scholar]
  20. Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. 1984a; Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. Journal of Medical Microbiology 17:79–89
    [Google Scholar]
  21. Lyon B. R., May J. W., Skurray R. A. 1984b; Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus. Molecular and General Genetics 193:554–556
    [Google Scholar]
  22. Lyon B. R., Tennent J. M., May J. W., Skurray R. A. 1986; Trimethoprim resistance encoded on a Staphylococcus aureus gentamicin resistance plasmid: Cloning and transposon mutagenesis. FEMS Microbiology Letters 33:189–192
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor; New York:
    [Google Scholar]
  24. Martel A., Masson M., Moreau N., LeGoffic F. 1983; Kinetic studies of aminoglycoside acetyltransferase and phosphotransferase from Staphylococcus aureus RPAL. Relationship between the two activities. European Journal of Biochemistry 133:515–521
    [Google Scholar]
  25. McDonnell R. W., Sweeney H. M., Cohen S. 1983; Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy 23:151–160
    [Google Scholar]
  26. Naidoo J., Noble W. C., Weissmann A., Dyke K. G. H. 1983; Gentamicin-resistant staphylococci: genetics of an outbreak in a dermatology department. Journal of Hygiene 91:7–16
    [Google Scholar]
  27. Pavillard R. 1982; Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Medical Journal of Australia 1:451–454
    [Google Scholar]
  28. Price E. H., Brain A., Dickson J. A. S. 1980; An outbreak of infection with a gentamicin and methicillin resistant Staphylococcus aureus in a neonatal unit. Journal of Hospital Infection 1:221–228
    [Google Scholar]
  29. Ray A., Skurray R. 1983; Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer. Plasmid 9:262–272
    [Google Scholar]
  30. Sanger F. Nucleotide sequence of bacteriophage oX174 DNA. Nature 265:687–695
    [Google Scholar]
  31. Scott D. F., Wood D. O., Brownell G. H., Carter M. J., Best G. K. 1978; Aminoglycoside modification by gentamicin-resistant isolates of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 13:641–644
    [Google Scholar]
  32. Shalita Murphy E., Novick R. P. 1980; Penicillinase plasmids of Staphylococcus aureus: structural and evolutionary relationships. Plasmid 3:291–311
    [Google Scholar]
  33. Smith G. E., Summers M. D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl- paper. Analytical Biochemistry 109:123–129
    [Google Scholar]
  34. Smith H. O. 1980; Recovery of DNA from gels. Methods in Enzymology 65:371–380
    [Google Scholar]
  35. Soussy C. J., Bouanchaud D. H., Fouace J., Dublanchet A., Duval J. 1975; A gentamycin resistance plasmid in Staphylococcus aureus. Annales de Microbiologie 126B:91–94
    [Google Scholar]
  36. Speller D. C. E. Epidemic infection by a gentamicin-resistant Staphylococcus aureus in three hospitals. Lancet 1:464–466
    [Google Scholar]
  37. Tennent J. M., Lyon B. R., Gillespie M. T., May J. W., Skurray R. A. 1985; Cloning and expression of Staphylococcus aureus plasmid-mediated quaternary ammonium resistance in Escherichia coli. Antimicrobial Agents and Chemotherapy 27:79–83
    [Google Scholar]
  38. Townsend D. E., Grubb W. B., Ashdown N. 1983; Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology 15:169–174
    [Google Scholar]
  39. Ubukata K., Yamashita N., Gotoh A., Konno M. 1984; Purification and characterization of aminoglycoside-modifying enzymes from Staphylococcus aureus and Staphylococcus epidermidis. Antimicrobial Agents and Chemotherapy 25:754–759
    [Google Scholar]
  40. Vogel L., Nathan C., Sweeney H. M., Kabins S. A., Cohen S. 1978; Infections due to gentamicin-resistant Staphylococcus aureus strain in a nursery for neonatal infants. Antimicrobial Agents and Chemotherapy 13:466–472
    [Google Scholar]
  41. Wood D. O., Carter M. J., Best G. K. 1977; Plasmid-mediated resistance to gentamicin in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 12:513–517
    [Google Scholar]
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