1887

Abstract

Summary

The clinical and bacteriological findings in five cases of septicaemia with cysteine-requiring isolates of are reported. Infections with these nutritionally-dependent organisms have been found previously in the urinary tract only, associated usually with chronic rather than acute conditions. The urinary tract was considered to be the source of the septicaemia in our patients and that site should be investigated when such strains are isolated from blood cultures. When first isolated the organisms characteristically form small translucent colonies on media deficient in appropriate growth factors. Their nutritional requirement for cysteine can be determined by a simple auxanographic technique, thereby enabling the appropriate supplementation of media necessary for reliable identification and antibiotic-sensitivity testing.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-4-379
1986-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/4/medmicro-22-4-379.html?itemId=/content/journal/jmm/10.1099/00222615-22-4-379&mimeType=html&fmt=ahah

References

  1. Bell S. M. 1975; The CDS disc method of antibiotic sensitivity testing (calibrated dichotomous sensitivity test). Pathology 7: Suppl 1–48
    [Google Scholar]
  2. Bevis T. D. 1968; A modified electrolyte deficient culture medium. Journal of Medical Laboratory Technology 25:38–41
    [Google Scholar]
  3. Borderon Horodniceanu T. 1978; Mctabolically deficient dwarf-colony mutants of Escherichia coli: deficiency and resistance to antibiotics of strains isolated from urine culture. Journal of Clinical Microbiology 8:629–634
    [Google Scholar]
  4. Brigham M. P., Stein W. H., Moore S. 1960; The concentrations of cysteine and cystine in human blood plasma. Journal of Clinical Investigation 39:1633–1638
    [Google Scholar]
  5. Cowan S. T. 1974; Cowan and Steel’s Manual for the identification of medical bacteria. 2nd edn Cambridge University Press; Cambridge: pp 103–107
    [Google Scholar]
  6. Dreyfuss Monty K. J. 1963; The biochemical characterization of cysteine-requiring mutants of Salmonella typhimurium. Journal of Biological Chemistry 238:1019–1024
    [Google Scholar]
  7. Gillespie W. A. 1952; Biochemical mutants of coliform bacilli in infections of the urinary tract. Journal of Pathology and Bacteriology 64:551–557
    [Google Scholar]
  8. Jones-Mortimer M. C. 1968; Positive control of sulphate reduction in Escherichia coli. Isolation, characterization and mapping of cysteineless mutants of E. coli K12. Biochemical Journal 110:589–595
    [Google Scholar]
  9. Leigh D. A., Williams J. D. 1964; Method for the detection of significant bacteriuria in large groups of patients. Journal of Clinical Pathology 17:498–503
    [Google Scholar]
  10. Mackey J. P., Sandys G. H. 1966; Diagnosis of urinary infections. British Medical Journal 1:11–73
    [Google Scholar]
  11. Pasternak C. A. 1962; Sulphate activation and its control in Escherichia coli and Bacillus subtilis. Biochemical Journal 85:44–49
    [Google Scholar]
  12. Tapsall J. W., Wilson E., Harper J. 1974; Thymine dependent strains of Escherichia coli selected by trimethoprim-sulphamethoxazole therapy. Pathology 6:161–167
    [Google Scholar]
  13. Tapsall J. W., Bell S. M., Smith D. D. 1975; Relevance of “significant bacteriuria" to aetiology and diagnosis of urinary tract infection. Lancet 2:637–639
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-4-379
Loading
/content/journal/jmm/10.1099/00222615-22-4-379
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error