Urine was collected twice weekly from one patient during a 25-month period. Escherichia coli harbouring a resistance plasmid, pUK28, which confers trimethoprim, ampicillin, sulphamethoxazole, spectinomycin and streptomycin resistance was identified in the urine. Carriage of strains containing plasmid pUK28 was observed during three separate periods which totalled 16 months, even though the patient did not receive antibacterial drug therapy for most of that time. The plasmid was able to acquire the genes responsible for mannose-resistant haemagglutination and these genes were increasingly associated with the plasmid towards the end of the study period.
AmyesS. G. B.,
DohertyC. J.,
YoungH.-K.1986; Changes in high-level trimethoprim resistance in hospital strains. In
IshigamiJ.
(ed) Recent advances in chemotherapy University of Tokyo Press; Tokyo: pp 403–404
BarthP. T.,
GrinterN. J.,
BradleyD. E.1976; Conjugal transfer system of plasmid RP4: Analysis by transposon 7 insertion. Journal of Bacteriology 133:43–52
HullR. A.,
GillR. E.,
HsuP.,
MinshewB. H.,
FalkowS.1981; Construction and expression of recombinant plasmids encoding Type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infection and Immunity 33:933–938
LjunghA.,
FarisA.,
WadströmT.1979; Hemagglutination by Escherichia coli in septicemia and urinary tract infections. Journal of Clinical Microbiology 10:477–481
ØrskovI.,
ØrskovF.1966; Episome-carried surface antigen K88 of Escherichia coli I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. Journal of Bacteriology 91:69–75
PenarandaM. E.,
MannM. B.,
EvansD. G.,
EvansD. J.1980; Transfer of an ST:LT:CFA/II plasmid into Escherichia coli K12 strain RR1 by contransformation with pSC301 plasmid DNA. FEMS Microbiology Letters 8:251–254
SmithH. W.,
LinggoodM. A.1972; Further observations on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains: the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. Journal of Medical Microbiology 5:243–250
Svanborg-EdénC.,
HagbergL.,
HansonL. A.,
KorhonenT.,
LefflerH.,
OilingS.1981; Adhesion of Escherichia coli in urinary tract infection. Ciba Foundation Symposium 80:161–187
Svanborg-EdénC.,
HanssonH. A.1978; Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells. Infection and Immunity 21:229–237