1887

Abstract

Summary

Some 10% of group-A streptococci have inhibitory activity against all nine strains (eight of them streptococci) in a set of indicators in an inhibitor-production typing (P-typing) scheme. This activity was associated with the concurrent synthesis of cell-associated proteinase by the streptococcal strain. Inhibitor production was prevented either by incubation of the test strain in conditions inimical to proeteinase production, e.g., at low temperature and alkaline H, or by addition to the medium of substances, such as glucose, iodoacetic acid, lincomycin, Congo red or trypan blue, that had an anti-proteinase effect. Inhibitory activity was not detectable in liquid cultures, but freeze-thaw extracts of cultures of group-A streptococcus strain A1013 on Gibco Columbia Agar Base (Gibco Diagnostics, Madison, WI, USA) had some inhibitory activity. The inhibitor was concentrated and partially purified, and the active agent was shown to be a high-mol.-wt cationic protein which was bactericidal for various bacteria in the logarithmic growth phase, including the homologous producer strain.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-22-3-257
1986-11-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/22/3/medmicro-22-3-257.html?itemId=/content/journal/jmm/10.1099/00222615-22-3-257&mimeType=html&fmt=ahah

References

  1. Bierbaum G., Sahl H. G. 1985; Induction of autolysis of staphylococci by the basic peptide antibiotics Pep 5 and nisin and their influence on the activity of autolytic enzymes. Archives of Microbiology 141:249–254
    [Google Scholar]
  2. Cleary P. P., Johnson Z., Wannamaker L. 1975; Genetic instability of M protein and serum opacity factor of group A streptococci: evidence suggesting extrachromosomal control. Infection and Immunity 12:109–118
    [Google Scholar]
  3. Elliott S. D. 1945; A proteolytic enzyme produced by group A streptococci with special reference to its effect on the type-specific M antigen. Journal of Experimental Medicine 81:573–592
    [Google Scholar]
  4. Elliott S. D. 1950; The crystallization and serological differentiation of a streptococcal proteinase and its precursor. Journal of Experimental Medicine 92:201–218
    [Google Scholar]
  5. Elliott S. D., Dole V. P. 1947; An inactive precursor of streptococcal proteinase. Journal of Experimental Medicine 85:305–320
    [Google Scholar]
  6. Fujimura S., Nakamura T. 1979; Sanguicin. a bacteriocin of oral Streptococcus sanguis . Antimicrobial Agents and Chemotherapy 16:262–265
    [Google Scholar]
  7. Gemmell C. G., Abdul Amir M. K. 1979; Effect of certain antibiotics on the formation of cellular and extracellular products by group A streptococci. In Parker M. T. (ed) Pathogenic streptococci Reedbooks Ltd; Chertsey, England: pp 67–68
    [Google Scholar]
  8. Hurst A. 1981; Nisin. Advances in Applied Microbiology 27:85–123
    [Google Scholar]
  9. Hynes W. L., Tagg J. R. 1985a; Production of broad-spectrum bacteriocin-like activity by group A streptococci of particular M-types. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene A 259:155–164
    [Google Scholar]
  10. Hynes W., Tagg J. R. 1985b; A simple plate assay for detection of group A streptococcus proteinase. Journal of Microbiological Methods 4:25–31
    [Google Scholar]
  11. Hynes W. L., Tagg J. R. 1986; Role of proteinase in the formation of inhibitory levels of haematin by group A streptococcus cultures on blood-containing media. Journal of Clinical Microbiology 23:929–933
    [Google Scholar]
  12. Ikeda T., Iwanami T., Watanabe C., McGhee J. M., Shiota T. 1982; Purification and certain properties of a bacteriocin from Streptococcus mutans . Infection and Immunity 35:861–868
    [Google Scholar]
  13. Johnson D. W., Tagg J. R., Wannamaker L. W. 1979; Production of a bacteriocine-like substance by group-A streptococci of M- type 4 and T-pattern 4. Journal of Medical Microbiology 12:413–427
    [Google Scholar]
  14. Nida K., Cleary P. P. 1983; Insertional inactivation of streptolysin S expression in Streptococcus pyogenes . Journal of Bacteriology 155:1156–1161
    [Google Scholar]
  15. Simpson W. J., Tagg J. R. 1983; M-type 57 group A streptococcus bacteriocin. Canadian Journal of Microbiology 29:1445–1451
    [Google Scholar]
  16. Simpson W. J., Tagg J. R. 1984; Survey of the plasmid content of group A streptococci. FEMS Microbiology Letters 23:195–199
    [Google Scholar]
  17. Starlinger P. 1980; IS elements and transposons. Plasmid 3:241–259
    [Google Scholar]
  18. Tagg J. R. 1984; Production of bacteriocin-like inhibitors by group A streptococci of nephritogenic M types. Journal of Clinical Microbiology 19:884–887
    [Google Scholar]
  19. Tagg J. R., Bannister L. V. 1979; “Fingerprinting” jS-haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors. Journal of Medical Microbiology 12:397–411
    [Google Scholar]
  20. Tagg J. R., Dajani A. S., Wannamaker L. W. 1975; Bacteriocin of a group B streptococcus: partial purification and characterization. Antimicrobial Agents and Chemotherapy 7:764–772
    [Google Scholar]
  21. Tagg J. R., Dajani A. S., Wannamaker L. W. 1976; Bacteriocins of gram-positive bacteria. Bacteriological Reviews 40:722–756
    [Google Scholar]
  22. Tagg J. R., Read R. S. D., McGiven A. R. 1973; Bacteriocin of a group A streptococcus: partial purification and properties. Antimicrobial Agents and Chemotherapy 4:214–221
    [Google Scholar]
  23. Taketo Y., Taketo A. 1982; Effects of trypan blue and related compounds on production and activity of streptolysin S. Zeitschrift fur Naturforschung 37C:385–389
    [Google Scholar]
  24. Travis J., Salvesen G. S. 1983; Human plasma proteinase inhibitors. Annual Review of Biochemistry 52:655–709
    [Google Scholar]
  25. Vogt A., Batsford S., Iturbe B., Garcia R. 1983; Cationic antigens in post-streptococcal glomerulonephritis. Clinical Nephrology 20:271–279
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-22-3-257
Loading
/content/journal/jmm/10.1099/00222615-22-3-257
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error